Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis

2017 ◽  
Vol 534 ◽  
pp. 46-48 ◽  
Author(s):  
Andrew Osahor ◽  
Karthik Deekonda ◽  
Choon-Weng Lee ◽  
Edmund Ui-Hang Sim ◽  
Aurelian Radu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Mammalian Cells ◽  
Sem Analysis ◽  
Rapid Preparation ◽  
Adherent Mammalian Cells ◽  
Scanning Electron

SEM analysis of fish scale cross sections: A technique study

1992 ◽  
Vol 50 (1) ◽  
pp. 744-745
Author(s):  
M.E. Lee ◽  
A. Moller ◽  
P.S.O. Fouche ◽  
I.G Gaigher
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cross Sections ◽  
Soft Tissues ◽  
Close Association ◽  
Sem Analysis ◽  
Fish Scale ◽  
Fish Scales ◽  
Micro Structures ◽  
Scanning Electron

Scanning electron microscopy of fish scales has facilitated the application of micro-structures to systematics. Electron microscopy studies have added more information on the structure of the scale and the associated cells, many problems still remain unsolved, because of our incomplete knowledge of the process of calcification. One of the main purposes of these studies has been to study the histology, histochemistry, and ultrastructure of both calcified and decalcified scales, and associated cells, and to obtain more information on the mechanism of calcification in the scales. The study of a calcified scale with the electron microscope is complicated by the difficulty in sectioning this material because of the close association of very hard tissue with very soft tissues. Sections often shatter and blemishes are difficult to avoid. Therefore the aim of this study is firstly to develop techniques for the preparation of cross sections of fish scales for scanning electron microscopy and secondly the application of these techniques for the determination of the structures and calcification of fish scales.

Development of Alginate Based Active Films Containing Turmeric Essential Oil

2020 ◽  
Vol 861 ◽  
pp. 378-382
Author(s):  
Sophoan Phal ◽  
Muhammad Rafiullah Khan ◽  
Pattarin Leelaphiwat ◽  
Vanee Chonhenchob
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Tensile Strength ◽  
Water Vapor ◽  
Essential Oil ◽  
Sem Analysis ◽  
Film Properties ◽  
Elongation At Break ◽  
Control Film ◽  
Scanning Electron

Sodium alginate based films containing turmeric oil (TEO) at different concentrations (1, 2 and 3%) were developed. The film with no TEO was used as control. Incorporation of TEO had the effects on the film properties. With increasing TEO concentrations, thickness, elongation at break, permeability of oxygen and water vapor of the films significantly (p ≤ 0.05) increased. Whereas moisture content, tensile strength and modulus of elasticity significantly (p ≤ 0.05) decreased. Scanning electron microscopy (SEM) analysis showed more numerous pores and rougher surface of the antifungal films than the control film.

Deepwater sculpin, Myoxocephalus thompsoni (Girard) from a Pleistocene nodule, Green Creek, Ontario, Canada

1979 ◽  
Vol 16 (8) ◽  
pp. 1621-1628 ◽  
Author(s):  
Donald E. Champagne ◽  
C. R. Harington ◽  
Don E. McAllister
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Habitat Preferences ◽  
Sem Analysis ◽  
Lake Basin ◽  
Lacustrine Environment ◽  
Clay Matrix ◽  
Summer Temperatures ◽  
Scanning Electron ◽  
Ultraviolet Lamp

A nodule containing the cranium of a deepwater sculpin, Myoxocephalus thompsoni (Girard), was discovered in Pleistocene Champlain Sea deposits at Green Creek, 10 km east of Ottawa, Ontario, Canada, and represents the first fossil reported for the species. It provides an additional basis for refuting derivation of the species from a post-Wisconsin marine submergence and suggests an origin at the beginning of the Wisconsin or earlier. Habitat preferences of the species favour an oligotrophic lacustrine environment with bottom summer temperatures below 8 °C in the Champlain Sea or nearby lake basin. The head length of the fossil is 28 mm, the estimated standard length 86 mm. Use of a longwave ultraviolet lamp with the fossil improved contrast between the bones and the clay matrix in photographs. Scanning electron microscopy (SEM) analysis of bone in the fossil failed to detect the presence of strontium.

Morphology of Lignin-Polyurethane Blends

1989 ◽  
Vol 153 ◽  
Author(s):  
D. Feldman ◽  
M.A. Lacasse
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Differential Scanning Calorimetry ◽  
Sem Analysis ◽  
Accelerated Weathering ◽  
Dsc Analysis ◽  
Mechanical Mixing ◽  
Scanning Calorimetry ◽  
Two Phases ◽  
Scanning Electron

AbstractThe morphology of blended polyurethane (PU) sealants was studied by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). The PU was modifided by mechanical mixing with various amounts of Lignin (L) to achieve a homogeneous blend. Specimens were subjected to control (C), accelerated weathering (AW), and natural weathering (NW) conditions. Results obtained from SEM analysis reveal an even distribution of L particles in the PU matrix. Furthermore, the SEM photomicrographs clearly emphasize the differing morphologies of the constituent phases. They also depict the differences in surface texture between control and aged specimens. The DSC analysis shows that the two phases are immiscible which is in agreement with observations by microscopy.

Arg-substituted VmCT1 analogs reveals promising candidate for the development of new antichagasic agent

Parasitology ◽  
2020 ◽  
Vol 147 (14) ◽  
pp. 1810-1818
Author(s):  
Cibele Nicolaski Pedron ◽  
Katielle Albuquerque Freire ◽  
Marcelo Der Torossian Torres ◽  
Dânya Bandeira Lima ◽  
Marília Lopes Monteiro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Flow Cytometry ◽  
Mammalian Cells ◽  
Rational Design ◽  
Membrane Damage ◽  
Action Mechanism ◽  
Promising Candidate ◽  
Cell Death Pathway ◽  
Scanning Electron

AbstractVmCT1 is an antimicrobial peptide (AMP) isolated from the venom of the scorpion Vaejovis mexicanus with antimicrobial, anticancer and antimalarial activities, which the rational design with Arg-substitution has yielded AMPs with higher antimicrobial activity than VmCT1. Chagas is a neglected tropical disease, becoming the development of new antichagasic agents is urgent. Thus, we aimed to evaluate the antichagasic effect of VmCT1 and three Arg-substituted analogues, as well their action mechanism. Peptides were tested against the epimastigote, trypomastigote, amastigote forms of Trypanossoma cruzi Y strain and against LLC-MK2 mammalian cells. The mechanism of action of these peptides was evaluated by means of flow cytometry and scanning electron microscopy. VmCT1 presented activity against all three forms of T. cruzi, with EC50 against trypomastigote forms of 1.37 μmol L−1 and selectivity index (SI) of 58. [Arg]3-VmCT1, [Arg]7-VmCT1 and [Arg]11-VmCT1 also showed trypanocidal effect, but [Arg]11-VmCT1 had the best effect, being able to decrease the EC50 against trypomastigote forms to 0.8 μmol L−1 and increase SI to 175. Necrosis was cell death pathway of VmCT1, as well [Arg]7-VmCT1 and [Arg]11-VmCT1, such as observed by membrane damage in flow cytometry analyses and scanning-electron-microscopy. In conclusion, [Arg]11-VmCT1 revealed promising as a candidate for new antichagasic therapeutics.

scholarly journals Deformation in cemented mudrock (Callovo–Oxfordian Clay) by microcracking, granular flow and phyllosilicate plasticity: insights from triaxial deformation, broad ion beam polishing and scanning electron microscopy

Solid Earth ◽  
2017 ◽  
Vol 8 (2) ◽  
pp. 291-305 ◽  
Author(s):  
Guillaume Desbois ◽  
Nadine Höhne ◽  
Janos L. Urai ◽  
Pierre Bésuelle ◽  
Gioacchino Viggiani
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ion Beam ◽  
Sem Analysis ◽  
Image Correlation ◽  
Grain Rotation ◽  
Show Evidence ◽  
Initial Fracture ◽  
Evolution Of Microstructure ◽  
Scanning Electron

Abstract. The macroscopic description of deformation and fluid flow in mudrocks can be improved by a better understanding of microphysical deformation mechanisms. Here we use a combination of scanning electron microscopy (SEM) and broad ion beam (BIB) polishing to study the evolution of microstructure in samples of triaxially deformed Callovo–Oxfordian Clay. Digital image correlation (DIC) was used to measure strain field in the samples and as a guide to select regions of interest in the sample for BIB–SEM analysis. Microstructures show evidence for dominantly cataclastic and minor crystal plastic mechanisms (intergranular, transgranular, intragranular cracking, grain rotation, clay particle bending) down to the nanometre scale. At low strain, the dilatant fabric contains individually recognisable open fractures, while at high strain the reworked clay gouge also contains broken non-clay grains and smaller pores than the undeformed material, resealing the initial fracture porosity.

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