Repetitive versus monomeric antigen presentation: direct visualization of antibody affinity and specificity

Werner Baschong; Lorenz Hasler; Markus Häner; Joerg Kistler; Ueli Aebi

doi:10.1016/j.jsb.2003.08.004

Repetitive versus monomeric antigen presentation: direct visualization of antibody affinity and specificity

Journal of Structural Biology ◽

10.1016/j.jsb.2003.08.004 ◽

2003 ◽

Vol 143 (3) ◽

pp. 258-262 ◽

Cited By ~ 18

Author(s):

Werner Baschong ◽

Lorenz Hasler ◽

Markus Häner ◽

Joerg Kistler ◽

Ueli Aebi

Keyword(s):

Antigen Presentation ◽

Direct Visualization ◽

Antibody Affinity

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T-Cell Activation Mediated by Facilitated Antigen Presentation (FAP) Is Directly Affected by Individual IgE Antibody Affinity

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2007.12.461 ◽

2008 ◽

Vol 121 (2) ◽

pp. S116-S116

Author(s):

N WILLUMSEN ◽

P WURTZEN ◽

J HOLM ◽

L CHRISTENSEN ◽

K LUND

Keyword(s):

T Cell ◽

Antigen Presentation ◽

T Cell Activation ◽

Cell Activation ◽

Antibody Affinity ◽

Ige Antibody

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Direct visualization of the dynamics of antigen presentation in human cells infected with cytomegalovirus revealed by antibodies mimicking TCR specificity

European Journal of Immunology ◽

10.1002/eji.200939875 ◽

2010 ◽

Vol 40 (6) ◽

pp. 1552-1565 ◽

Cited By ~ 24

Author(s):

Oryan Makler ◽

Kfir Oved ◽

Nir Netzer ◽

Dana Wolf ◽

Yoram Reiter

Keyword(s):

Antigen Presentation ◽

Human Cells ◽

Direct Visualization

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Direct visualization of phase-separated domains in lipid membranes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082315 ◽

1978 ◽

Vol 36 (2) ◽

pp. 290-291

Author(s):

S. W. Hui ◽

T. P. Stewart

Keyword(s):

Lipid Membranes ◽

Structural Information ◽

Freeze Fracture ◽

Biological Molecules ◽

Vacuum Drying ◽

Direct Visualization ◽

X Ray Diffraction ◽

Electron Microscopic ◽

X Ray ◽

Hydrocarbon Chains

Direct electron microscopic study of biological molecules has been hampered by such factors as radiation damage, lack of contrast and vacuum drying. In certain cases, however, the difficulties may be overcome by using redundent structural information from repeating units and by various specimen preservation methods. With bilayers of phospholipids in which both the solid and fluid phases co-exist, the ordering of the hydrocarbon chains may be utilized to form diffraction contrast images. Domains of different molecular packings may be recgnizable by placing properly chosen filters in the diffraction plane. These domains would correspond to those observed by freeze fracture, if certain distinctive undulating patterns are associated with certain molecular packing, as suggested by X-ray diffraction studies. By using an environmental stage, we were able to directly observe these domains in bilayers of mixed phospholipids at various temperatures at which their phases change from misible to inmissible states.

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