Sperm and seminal plasma differentially regulate cytokine and chemokine protein expression by human cervical epithelial cells

2010 ◽  
Vol 86 (1) ◽  
pp. 68
Author(s):  
D.J. Sharkey ◽  
S.A. Robertson
Keyword(s):  
Epithelial Cells ◽  
Protein Expression ◽  
Seminal Plasma ◽  
Cervical Epithelial Cells

scholarly journals 229.Seminal plasma TGFβ activates pro-inflammatory cytokine synthesis in human cervical epithelial cells

2004 ◽  
Vol 16 (9) ◽  
pp. 229 ◽  
Author(s):  
D. J. Sharkey ◽  
S. A. Robertson
Keyword(s):  
Epithelial Cells ◽  
Seminal Plasma ◽  
Model Systems ◽  
Human Seminal Plasma ◽  
Neutralising Antibodies ◽  
Active Constituents ◽  
Gm Csf ◽  
Cytokine Synthesis ◽  
Cervical Epithelial Cells

Exposure to semen at intercourse in women elicits an inflammation-like response characterised by recruitment of inflammatory cells and expression of pro-inflammatory cytokines including GM-CSF, interleukin-6 (IL-6) and IL-8 (1). Studies in animal models have implicated TGFβ as the major active moiety in seminal plasma, and we have shown previously that TGFβ1 and TGFβ3 are present in high concentrations in human seminal plasma (>100 ng/mL), while TGFβ2 is less abundant. To investigate the physiological significance of each of the three TGFβ isoforms as pro-inflammatory agents in human seminal plasma, we have established in vitro model systems to measure human cervical cell cytokine synthesis. Primary cervical epithelial cells prepared from ectocervix of hysterectomy tissues or transformed Ect1 cells were incubated for 12 h with human recombinant TGFβ (isoforms 1, 2 or 3) or with seminal plasma in the presence or absence of isoform-specific TGFβ neutralising antibodies. Epithelial cell supernatants were recovered 24 h later and supernatants were analysed by commercial ELISA to quantify GM-CSF, IL-6 and IL-8 production. Each of the three TGFβ isoforms mimicked seminal plasma and were comparable in their capacity to stimulate >10-fold increases in both GM-CSF and IL-6 expression in a dose-responsive manner. In contrast, unlike seminal plasma none of the TGFβ isoforms induced IL-8 expression. Addition of neutralising antibodies to TFGβ1, TGFβ2 and TGFβ3 each effected >50% reduction in the ability of seminal plasma to induce GM-CSF and IL-6, but did not impair seminal plasma-stimulated IL-8 production. Together these data show that TGFβ1, TGFβ2 and TGFβ3 are major active constituents of seminal plasma, acting to elicit GM-CSF and IL-6 production in cervical epithelial cells. However, TGFβ does not fully account for the pro-inflammatory effects of human seminal plasma, and other active constituents remain to be identified. (1) D. J. Sharkey et al. (2003) Proc. SRB.

Activation of telomerase by seminal plasma in malignant and normal cervical epithelial cells

2011 ◽  
Vol 225 (2) ◽  
pp. 203-211 ◽  
Author(s):  
Li Liu ◽  
Cheng Liu ◽  
Fenglan Lou ◽  
Guiyu Zhang ◽  
Xizhi Wang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Seminal Plasma ◽  
Cervical Epithelial Cells

scholarly journals Thioredoxin Reductase Is Essential for Protection ofNeisseria gonorrhoeaeagainst Killing by Nitric Oxide and for Bacterial Growth during Interaction with Cervical Epithelial Cells

2009 ◽  
Vol 199 (2) ◽  
pp. 227-235 ◽  
Author(s):  
Adam J. Potter ◽  
Stephen P. Kidd ◽  
Jennifer L. Edwards ◽  
Megan L. Falsetta ◽  
Michael A. Apicella ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Bacterial Growth ◽  
Thioredoxin Reductase ◽  
Cervical Epithelial Cells
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