scholarly journals Thioredoxin Reductase Is Essential for Protection ofNeisseria gonorrhoeaeagainst Killing by Nitric Oxide and for Bacterial Growth during Interaction with Cervical Epithelial Cells

2009 ◽  
Vol 199 (2) ◽  
pp. 227-235 ◽  
Author(s):  
Adam J. Potter ◽  
Stephen P. Kidd ◽  
Jennifer L. Edwards ◽  
Megan L. Falsetta ◽  
Michael A. Apicella ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Bacterial Growth ◽  
Thioredoxin Reductase ◽  
Cervical Epithelial Cells

scholarly journals Esterase D Is Essential for Protection ofNeisseria gonorrhoeaeagainst Nitrosative Stress and for Bacterial Growth during Interaction with Cervical Epithelial Cells

2009 ◽  
Vol 200 (2) ◽  
pp. 273-278 ◽  
Author(s):  
Adam J. Potter ◽  
Stephen P. Kidd ◽  
Jennifer L. Edwards ◽  
Megan L. Falsetta ◽  
Michael A. Apicella ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Bacterial Growth ◽  
Nitrosative Stress ◽  
Cervical Epithelial Cells

scholarly journals Involvement of the Toll-Like Receptor/Nitric Oxide Signaling Pathway in the Pathogenesis of Cervical Cancer Caused by High-Risk Human Papillomavirus Infection

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Jie Li ◽  
Heping Rao ◽  
Chang’e Jin ◽  
Jinrong Liu
Keyword(s):  
Nitric Oxide ◽  
Cervical Cancer ◽  
Human Papillomavirus ◽  
High Risk ◽  
Epithelial Cells ◽  
Signaling Pathway ◽  
Cervical Canal ◽  
Toll Like Receptor ◽  
No Signaling ◽  
Cervical Epithelial Cells

Human papillomavirus (HPV) can activate Toll-like receptor (TLR)/nitric oxide (NO) signaling pathways; however, whether the TLR/NO pathway is involved in cervical cancer caused by high-risk HPV (HR-HPV) remains unclear. In this study, 43 HR-HPV-positive patients with cervical cancer (CC group), 39 HR-HPV-positive patients with a healthy cervix (HR-HPV group), and 33 HR-HPV-negative controls were recruited. NO concentration in cervical canal and expression of inducible NO synthase (iNOS) in cervical tissues were detected. Expressions of key TLR/NO pathway genes (TLR3/4/7/8, NF-κB p65, and iNOS) in cervical epithelial cells were detected by quantitative reverse transcription PCR. Expressions of TLR4, NF-κB p65, and iNOS in CaSki, HeLa, and C33a cells were determined by Western blot. NO concentration in cervical canal of CC group was significantly higher than in other groups (P<0.05). Positive rates of iNOS in cervical tissues were 72.1%, 28.2%, and 3.1% in the CC group, HR-HPV group, and controls, respectively (P<0.05). Levels of TLR3, TLR4, TLR7, TLR8, NF-κB p65, and iNOS in cervical epithelial cells were higher in CC group than in other groups (P<0.05). Both mRNA and protein levels of TLR4, NF-κB p65, and iNOS were higher in HPV-positive HeLa and CaSki cells than in HPV-negative C33a cells (P<0.05). Together, these results suggest that TLR/NO signaling pathway may be involved in pathogenesis of cervical cancer caused by HR-HPV.

Nitric oxide increases IL-8 gene transcription and mRNA stability to enhance IL-8 gene expression in lung epithelial cells

2004 ◽  
Vol 287 (4) ◽  
pp. L764-L773 ◽  
Author(s):  
Loretta Sparkman ◽  
Vijayakumar Boggaram
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Protein Kinase ◽  
Epithelial Cells ◽  
Gene Transcription ◽  
Mrna Stability ◽  
Inflammatory Responses ◽  
Lung Epithelial Cells ◽  
Mrna Levels ◽  
Lung Epithelial

Interleukin (IL)-8, a C-X-C chemokine, is a potent chemoattractant and an activator for neutrophils, T cells, and other immune cells. The airway and respiratory epithelia play important roles in the initiation and modulation of inflammatory responses via production of cytokines and surfactant. The association between elevated levels of nitric oxide (NO) and IL-8 in acute lung injury associated with sepsis, acute respiratory distress syndrome, respiratory syncytial virus infection in infants, and other inflammatory diseases suggested that NO may play important roles in the control of IL-8 gene expression in the lung. We investigated the role of NO in the control of IL-8 gene expression in H441 lung epithelial cells. We found that a variety of NO donors significantly induced IL-8 mRNA levels, and the increase in IL-8 mRNA was associated with an increase in IL-8 protein. NO induction of IL-8 mRNA was due to increases in IL-8 gene transcription and mRNA stability. NO induction of IL-8 mRNA levels was not inhibited by 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one and KT-5823, inhibitors of soluble guanylate cyclase and protein kinase G, respectively, and 8-bromo-cGMP did not increase IL-8 mRNA levels. This indicated that NO induces IL-8 mRNA levels independently of changes in the intracellular cGMP levels. NO induction of IL-8 mRNA was significantly reduced by inhibitors of extracellular regulated kinase and protein kinase C. IL-8 induction by NO was also reduced by hydroxyl radical scavengers such as dimethyl sulfoxide and dimethylthiourea, indicating the involvement of hydroxyl radicals in the induction process. NO induction of IL-8 gene expression could be a significant contributing factor in the initiation and induction of inflammatory response in the respiratory epithelium.

Nitric Oxide as a Second Messenger in Phagocytosis by Cultured Retinal Pigment Epithelial Cells

2000 ◽  
Vol 32 (4) ◽  
pp. 138-142 ◽  
Author(s):  
Jun-ichi Kogishi ◽  
Masayuki Akimoto ◽  
Michiko Mandai ◽  
Shoji Kuriyama ◽  
Michael O. Hall ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Second Messenger ◽  
Retinal Pigment Epithelial Cells ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells

Polymorphism of TP53 gene and the risk of high human papillomavirus load in cervical epithelial cells

Gene Reports ◽  
2022 ◽  
Vol 26 ◽  
pp. 101456
Author(s):  
Abbas Hadi Albosale ◽  
Olga Andreevna Garbuzova ◽  
Konstantin Alekseevich Kovalenko ◽  
Elena Vladimirovna Mashkina
Keyword(s):  
Human Papillomavirus ◽  
Epithelial Cells ◽  
Tp53 Gene ◽  
Cervical Epithelial Cells

Characterization of Nitric Oxide (NO)-Induced Cell Death in Lung Epithelial Cells

2004 ◽  
Vol 56 (2) ◽  
pp. 187
Author(s):  
Wha Shim Yong ◽  
Youn Seup Kim ◽  
Jae Seuk Park ◽  
Young Koo Jee ◽  
Kye Young Lee
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Lung Epithelial

scholarly journals Differential regulation of IFNα, IFNβ and IFNε gene expression in human cervical epithelial cells

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jennifer Couret ◽  
Carley Tasker ◽  
Jaeha Kim ◽  
Tiina Sihvonen ◽  
Saahil Fruitwala ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Differential Regulation ◽  
Cervical Epithelial Cells
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