scholarly journals Zinc and magnesium ions synergistically inhibit superoxide generation by cultured human neutrophils—a promising candidate formulation for amnioinfusion fluid

2010 ◽  
Vol 85 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Toshiyuki Uchida ◽  
Hiroaki Itoh ◽  
Yuki Nakamura ◽  
Yukiko Kobayashi ◽  
Kyuya Hirai ◽  
...  
Keyword(s):  
Human Neutrophils ◽  
Magnesium Ions ◽  
Superoxide Generation ◽  
Promising Candidate

The influence of chlorhexidine on superoxide generation by induced human neutrophils

1987 ◽  
Vol 22 (6) ◽  
pp. 445-450 ◽  
Author(s):  
W. L. Gabler ◽  
W. W. Bullock ◽  
H. R. Creamer
Keyword(s):  
Human Neutrophils ◽  
Superoxide Generation

Protective action of propionyl-l-carnitine on human neutrophils superoxide generation

1990 ◽  
Vol 22 ◽  
pp. S117
Author(s):  
Marco Tubaro ◽  
Achille Gaspardone ◽  
Giovanni Cavallo ◽  
Anna M Chessa ◽  
Claudio Santiangeli ◽  
...  
Keyword(s):  
Protective Action ◽  
Human Neutrophils ◽  
Superoxide Generation

scholarly journals Response of neutrophils to stimulus infusion: differential sensitivity of cytoskeletal activation and oxidant production.

1988 ◽  
Vol 107 (3) ◽  
pp. 951-958 ◽  
Author(s):  
G M Omann ◽  
L A Sklar
Keyword(s):  
Inflammatory Responses ◽  
Receptor Occupancy ◽  
Differential Sensitivity ◽  
Human Neutrophils ◽  
Light Scatter ◽  
Superoxide Generation ◽  
Rate Of Increase ◽  
A Cell ◽  
Oxidant Production ◽  
Formyl Peptides

The response of human neutrophils to N-formyl peptides were studied under conditions where ligand binding was controlled by infusing a cell suspension with the peptide over a time period comparable to the normal half-time for binding. Receptor occupancy was measured in real time with a fluorescently labeled peptide using flow cytometry. This binding was approximated by a simple reversible model using typical on (7 X 10(8) M- min-1) and off (0.35/min) rate constants and the infusion rates (0.02-0.2 nM/min). Under conditions of stimulus infusion intracellular calcium elevation, superoxide generation, and right angle light scatter and F-actin formation were measured. As the infusion rate was decreased into the range of 10 pM/min, lowering the rate of increase of receptor occupancy to approximately 0.5% per min, the calcium and right angle light scatter responses elongated in time and decreased in magnitude. Superoxide generation decreased below infusion rates of approximately 100 pM/min (occupancy increasing at a rate in the range of 5% per min). This behavior could contribute to differences between chemotactic responses, which appear to require low rates of receptor occupancy over long periods, and bactericidal or inflammatory responses (free radical generation and degranulation), which require bursts of occupancy of several percent of the receptors.

scholarly journals Regulation of superoxide generation by myeloperoxidase during the respiratory burst of human neutrophils

1986 ◽  
Vol 237 (2) ◽  
pp. 601-604 ◽  
Author(s):  
S W Edwards ◽  
T F Swan
Keyword(s):  
Respiratory Burst ◽  
Cytochalasin B ◽  
Superoxide Radical ◽  
Human Neutrophils ◽  
Second Phase ◽  
Chemotactic Peptide ◽  
O2 Uptake ◽  
Superoxide Generation ◽  
Lower Magnitude

The role of myeloperoxidase in the regulation of the respiratory burst of human neutrophils activated by the chemotactic peptide (N-formyl-L-methionyl-L-leucyl-L-phenylalanine) plus cytochalasin B was determined by using anti-(human myeloperoxidase) antibody. The respiratory burst activated under these conditions consisted of an initial (1-2 min) phase with high rates of O2 uptake, luminol-dependent chemiluminescence and superoxide radical (O2-.) generation and a second, more sustained, phase of lower magnitude of chemiluminescence and O2 uptake: O2-. generation did not occur during this second phase. In cell suspensions stimulated in the presence of anti-(human myeloperoxidase) antibody, the magnitude of the initial phase of both O2 uptake and O2-. generation was unaffected, but these high rates were maintained over much longer periods than in control suspensions. It is therefore proposed that a product of myeloperoxidase normally regulates the duration of O2-. generation during the respiratory burst, possibly by inhibition of NADPH oxidase.

Failure of Neutrophils to Recover Their Ability to Produce Superoxide after Stunning by a Conventional, Acidic, Lactate-Based Peritoneal Dialysis Solution

1994 ◽  
Vol 17 (4) ◽  
pp. 191-194 ◽  
Author(s):  
T.S. Ing ◽  
A.W. Yu ◽  
P.V. Podila ◽  
F.Q. Zhou ◽  
E.W. Kun ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Extracellular Ph ◽  
Human Neutrophils ◽  
Superoxide Generation ◽  
Dialysis Solution ◽  
Peritoneal Dialysis Solution ◽  
Dialysis Solutions ◽  
Peritoneal Dialysis Solutions

Exposure of human neutrophils to conventional, acidic, lactate-based peritoneal dialysis solutions for 5 minutes results in a depression of superoxide generation. In spite of restoration of extracellular pH to 7.4, these stunned cells failed to recover their ability to generate the anion after a period of an hour.

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