Effect of five triterpenoid compounds isolated from root bark of Aralia elata on stimulus-induced superoxide generation, tyrosyl or serine/threonine phosphorylation and translocation of p47phox, p67phox, and rac to cell membrane in human neutrophils

2006 ◽  
Vol 446 (1) ◽  
pp. 84-90 ◽  
Author(s):  
Sachiko N. Yagi-Chaves ◽  
Gang Liu ◽  
Koichi Yamashita ◽  
Masanobu Manabe ◽  
Shao-Jiang Song ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Human Neutrophils ◽  
Root Bark ◽  
Superoxide Generation ◽  
Aralia Elata

scholarly journals Effects of Bioflavonoids from Taxus Media var. Hicksii on Superoxide Generation, Phosphorylation of Proteins and Translocation of Cytosolic Compounds to the Cell Membrane in Human Neutrophils

2020 ◽  
Author(s):  
Chongning Lv ◽  
Xueni Niu ◽  
Jincai Lu
Keyword(s):  
Cell Membrane ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Human Neutrophils ◽  
Scavenging Activity ◽  
Superoxide Generation ◽  
Remarkable Effect ◽  
Taxus Media ◽  
Phosphorylation Of Proteins ◽  
Depth Study

In present work, the effects of bioflavonoids (ginkgetin and sciadopitysin) on stimulus-induced superoxide generation, tyrosyl and serine/threonine phosphorylation of proteins in human neutrophils, and the translocation of cytosolic compounds (p47phox, p67phox and Rac) to cell membrane were studied, which were isolated from the needles of Taxus media var. Hicksii. Meanwhile, three normal flavonoids (apigenin, quercetin and isoquercetin) were involved as contrasts. The results indicated that ginkgetin and sciadopitysin were capable of concentration-dependently inhibitory effects on the superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP), arachidonic acid (AA) and phorbol-12-myristate 13-acetate (PMA). And they also suppressed fMLP- and AA- induced tyrosyl or PMA-induced serine/threonine phosphorylation and the translocation of cytosolic compounds (p47phox, p67phox and Rac) to cell membrane, which were in parallel with the suppression of the stimulus-induced superoxide generation. The effect of these compounds on the radical-scavenging was also investigated. Ginkgetin and sciadopitysin did not show remarkable effect on DPPH radical-scavenging activity, and they didn’t display the radical-scavenging activity on superoxide anion generated by phenagine methoxysulfate (PMS)-NADH system. Apparently, ginkgetin and sciadopitysin had great performance in pharmacological value and they are worthy of in-depth study.

scholarly journals Zinc and magnesium ions synergistically inhibit superoxide generation by cultured human neutrophils—a promising candidate formulation for amnioinfusion fluid

2010 ◽  
Vol 85 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Toshiyuki Uchida ◽  
Hiroaki Itoh ◽  
Yuki Nakamura ◽  
Yukiko Kobayashi ◽  
Kyuya Hirai ◽  
...  
Keyword(s):  
Human Neutrophils ◽  
Magnesium Ions ◽  
Superoxide Generation ◽  
Promising Candidate
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