Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration

2019 ◽  
Vol 195 ◽  
pp. 98-113 ◽  
Author(s):  
Zhen Dong ◽  
Dawn Coates ◽  
Qingxiu Liu ◽  
Hongmei Sun ◽  
Chunyi Li
Keyword(s):  
Stem Cells ◽  
Proteomic Analysis ◽  
Deer Antler ◽  
Quantitative Proteomic Analysis ◽  
Organ Regeneration

Defining pluripotent stem cells through quantitative proteomic analysis

2011 ◽  
Vol 8 (1) ◽  
pp. 29-42 ◽  
Author(s):  
Sonja Reiland ◽  
Ghasem Hosseini Salekdeh ◽  
Jeroen Krijgsveld
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Proteomic Analysis ◽  
Quantitative Proteomic Analysis

scholarly journals SWATH-MS Quantitative Proteomic Analysis of Deer Antler from Two Regenerating and Mineralizing Sections

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 679
Author(s):  
María López-Pedrouso ◽  
José M. Lorenzo ◽  
Tomás Landete-Castillejos ◽  
Louis Chonco ◽  
Francisco Javier Pérez-Barbería ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Proteomic Analysis ◽  
Redox Regulation ◽  
Growth Period ◽  
Tissue Growth ◽  
Blood Proteins ◽  
Deer Antler ◽  
Quantitative Proteomic Analysis ◽  
Antler Growth ◽  
Key Factor

Antlers are the only organ in the mammalian body that regenerates each year. They can reach growth rates of 1–3 cm/day in length and create more than 20 cm2/day of skin in the antler tips (their growth centers). Previous proteomic studies regarding antlers have focused on antler growth centers (tips) compared to the standard bone to detect the proteins involved in tissue growth. However, proteins of cell differentiation and regeneration will be more accurately detected considering more growing tissues. Thus, we set out to compare proteins expressed in antler tips (the highest metabolism rate and cell differentiation) vs. middle sections (moderate cell growth involving bone calcification), using ribs as controls. Samples were obtained in mid-June with antlers’ phenology corresponding to the middle of their growth period. Quantitative proteomic analysis identified 259 differentially abundant proteins mainly associated with antioxidant metabolic mechanisms, protein formation and Wnt signalling pathway, meanwhile, the mid antler section was linked to blood proteins. The high metabolic rate and subsequent risk of oxidative stress also seem to have resulted in strong antioxidant mechanisms. These results suggest that redox regulation of proteins is a key factor in the model of deer antler regeneration.

scholarly journals Quantitative Proteomic Analysis of Primitive Neural Stem Cells from LRRK2 G2019S-Associated Parkinson’s Disease Patient-Derived iPSCs

Life ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 331
Author(s):  
Hyuna Sim ◽  
Ji-Hye Seo ◽  
Jumi Kim ◽  
Minyoung Oh ◽  
Joo-Eun Lee ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Proteomic Analysis ◽  
Disease Patient ◽  
Experimental System ◽  
Model System ◽  
Neural Cells ◽  
Quantitative Proteomic Analysis ◽  
G2019s Mutation

Parkinson’s disease (PD) is a common neurodegenerative disease, causing movement defects. The incidence of PD is constantly increasing and this disease is still incurable. Thus, understanding PD pathophysiology would be pivotal for the development of PD therapy, and various PD models have thus been already developed. Through recent advances in reprogramming techniques, a primitive neural stem cell (pNSC) derived from PD patient induced pluripotent stem cells (iPSCs) could be potentially used as a reproducible and reliable experimental system to analyze the effect of the leucine-rich repeat kinase 2 G2019S mutation (LK2GS) in neural cells. Here, we investigated the advantages of such a model system through quantitative proteomic analysis of pNSCs from normal control iPSCs and familial PD patient iPSCs harboring LK2GS. We confirmed that the expression of molecules known to be involved in PD pathogenesis, such as oxidative stress-, cell adhesion-, and cytoskeleton-related proteins, were altered in the LK2GS pNSC. In addition, we showed that down-regulation of Ku80, which was found in the proteomic analysis with LK2GS pNSCs, resulted in apoptosis induced by DNA damage response. Taken together, we suggest that pNSCs from PD iPSCs could provide a reliable and useful model system to study PD. Moreover, the highly expandable pNSC is suitable for multi-omics approaches to understand PD pathologies and discover therapeutic targets for PD.

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