scholarly journals Top-down analysis of immunoglobulin G isotypes 1 and 2 with electron transfer dissociation on a high-field Orbitrap mass spectrometer

2017 ◽  
Vol 159 ◽  
pp. 67-76 ◽  
Author(s):  
Luca Fornelli ◽  
Daniel Ayoub ◽  
Konstantin Aizikov ◽  
Xiaowen Liu ◽  
Eugen Damoc ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Mass Spectrometer ◽  
Immunoglobulin G ◽  
High Field ◽  
Electron Transfer Dissociation ◽  
Top Down ◽  
Orbitrap Mass Spectrometer

scholarly journals Dual Electrospray Ion Source for Electron-Transfer Dissociation on a Hybrid Linear Ion Trap−Orbitrap Mass Spectrometer

2007 ◽  
Vol 79 (20) ◽  
pp. 7916-7919 ◽  
Author(s):  
D. Keith Williams, ◽  
Graeme C. McAlister ◽  
David M. Good ◽  
Joshua J. Coon ◽  
David C. Muddiman
Keyword(s):  
Electron Transfer ◽  
Mass Spectrometer ◽  
Electron Transfer Dissociation ◽  
Ion Trap ◽  
Ion Source ◽  
Linear Ion Trap ◽  
Orbitrap Mass Spectrometer

scholarly journals Implementation of Electron-Transfer Dissociation on a Hybrid Linear Ion Trap−Orbitrap Mass Spectrometer

2007 ◽  
Vol 79 (10) ◽  
pp. 3525-3534 ◽  
Author(s):  
Graeme C. McAlister ◽  
Doug Phanstiel ◽  
David M. Good ◽  
W. Travis Berggren ◽  
Joshua J. Coon
Keyword(s):  
Electron Transfer ◽  
Mass Spectrometer ◽  
Electron Transfer Dissociation ◽  
Ion Trap ◽  
Linear Ion Trap ◽  
Orbitrap Mass Spectrometer

scholarly journals A Proteomics Grade Electron Transfer Dissociation-Enabled Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer

2008 ◽  
Vol 7 (8) ◽  
pp. 3127-3136 ◽  
Author(s):  
Graeme C. McAlister ◽  
W. Travis Berggren ◽  
Jens Griep-Raming ◽  
Stevan Horning ◽  
Alexander Makarov ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Mass Spectrometer ◽  
Electron Transfer Dissociation ◽  
Ion Trap ◽  
Linear Ion Trap ◽  
Orbitrap Mass Spectrometer

scholarly journals Rapid and Deep Proteomes by Faster Sequencing on a Benchtop Quadrupole Ultra-High-Field Orbitrap Mass Spectrometer

2014 ◽  
Vol 13 (12) ◽  
pp. 6187-6195 ◽  
Author(s):  
Christian D. Kelstrup ◽  
Rosa R. Jersie-Christensen ◽  
Tanveer S. Batth ◽  
Tabiwang N. Arrey ◽  
Andreas Kuehn ◽  
...  
Keyword(s):  
Mass Spectrometer ◽  
High Field ◽  
Ultra High Field ◽  
Orbitrap Mass Spectrometer

scholarly journals Increasing the Productivity of Glycopeptides Analysis by Using Higher-Energy Collision Dissociation-Accurate Mass-Product-Dependent Electron Transfer Dissociation

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Julian Saba ◽  
Sucharita Dutta ◽  
Eric Hemenway ◽  
Rosa Viner
Keyword(s):  
Electron Transfer ◽  
Posttranslational Modifications ◽  
Electron Transfer Dissociation ◽  
Dynamic Range ◽  
Ion Trap ◽  
Accurate Mass ◽  
Energy Collision ◽  
User Input ◽  
Orbitrap Mass Spectrometer ◽  
Acquisition Strategy

Currently, glycans are attracting attention from the scientific community as potential biomarkers or as posttranslational modifications (PTMs) of therapeutic proteins. However, structural characterization of glycoproteins and glycopeptides remains analytically challenging. Here, we report on the implementation of a novel acquisition strategy termed higher-energy collision dissociation-accurate mass-product-dependent electron transfer dissociation (HCD-PD-ETD) on a hybrid linear ion trap-orbitrap mass spectrometer. This acquisition strategy uses the complementary fragmentations of ETD and HCD for glycopeptides analysis in an intelligent fashion. Furthermore, the approach minimizes user input for optimizing instrumental parameters and enables straightforward detection of glycopeptides. ETD spectra are only acquired when glycan oxonium ions from MS/MS HCD are detected. The advantage of this approach is that it streamlines data analysis and improves dynamic range and duty cycle. Here, we present the benefits of HCD-PD-ETD relative to the traditional alternating HCD/ETD for a trainer set containing twelve-protein mixture with two glycoproteins: human serotransferrin, ovalbumin and contaminations of two other: bovine alpha 1 acid glycoprotein (bAGP) and bovine fetuin.

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