Combination of a discovery LC–MS/MS analysis and a label-free quantification for the characterization of an epithelial–mesenchymal transition signature

2014 ◽  
Vol 110 ◽  
pp. 183-194 ◽  
Author(s):  
Jordane Biarc ◽  
Philippe Gonzalo ◽  
Ivan Mikaelian ◽  
Laurent Fattet ◽  
Mathieu Deygas ◽  
...  
Keyword(s):  
Epithelial Mesenchymal Transition ◽  
Label Free ◽  
Mesenchymal Transition ◽  
Label Free Quantification ◽  
Ms Analysis ◽  
Free Quantification

scholarly journals Spectral Clustering Improves Label-Free Quantification of Low-Abundant Proteins

2019 ◽  
Vol 18 (4) ◽  
pp. 1477-1485 ◽  
Author(s):  
Johannes Griss ◽  
Florian Stanek ◽  
Otto Hudecz ◽  
Gerhard Dürnberger ◽  
Yasset Perez-Riverol ◽  
...  
Keyword(s):  
Spectral Clustering ◽  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification

scholarly journals Mass Spectrometry-Based Proteomic Characterization of Cutaneous Melanoma Ectosomes Reveals the Presence of Cancer-Related Molecules

2020 ◽  
Vol 21 (8) ◽  
pp. 2934 ◽  
Author(s):  
Magdalena Surman ◽  
Sylwia Kędracka-Krok ◽  
Dorota Hoja-Łukowicz ◽  
Urszula Jankowska ◽  
Anna Drożdż ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Protein Content ◽  
Cell Lines ◽  
Cutaneous Melanoma ◽  
Epithelial Mesenchymal Transition ◽  
Mesenchymal Transition ◽  
Starting Point ◽  
Novel Biomarkers

Cutaneous melanoma (CM) is an aggressive type of skin cancer for which effective biomarkers are still needed. Recently, the protein content of extracellular vesicles (ectosomes and exosomes) became increasingly investigated in terms of its functional role in CM and as a source of novel biomarkers; however, the data concerning the proteome of CM-derived ectosomes is very limited. We used the shotgun nanoLC–MS/MS approach to the profile protein content of ectosomes from primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cell lines. Additionally, the effect exerted by CM ectosomes on recipient cells was assessed in terms of cell proliferation (Alamar Blue assay) and migratory properties (wound healing assay). All cell lines secreted heterogeneous populations of ectosomes enriched in the common set of proteins. A total of 1507 unique proteins were identified, with many of them involved in cancer cell proliferation, migration, escape from apoptosis, epithelial–mesenchymal transition and angiogenesis. Isolated ectosomes increased proliferation and motility of recipient cells, likely due to the ectosomal transfer of different cancer-promoting molecules. Taken together, these results confirm the significant role of ectosomes in several biological processes leading to CM development and progression, and might be used as a starting point for further studies exploring their diagnostic and prognostic potential.

Global Analysis of Protein Expression in A549 Cells After Prolonged Nicotine Exposure by Using Label-free Quantification

2021 ◽  
Vol 41 (8) ◽  
pp. 3833-3842
Author(s):  
SASIKARN KOMKLEOW ◽  
CHURAT WEERAPHAN ◽  
DARANEE CHOKCHAICHAMNANKIT ◽  
PAPADA CHAISURIYA ◽  
CHRIS VERATHAMJAMRAS ◽  
...  
Keyword(s):  
Protein Expression ◽  
Global Analysis ◽  
A549 Cells ◽  
Nicotine Exposure ◽  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification

Label-Free Quantification of Anticancer Drug Imatinib in Human Plasma with Surface Enhanced Raman Spectroscopy

2018 ◽  
Vol 90 (21) ◽  
pp. 12670-12677 ◽  
Author(s):  
Stefano Fornasaro ◽  
Alois Bonifacio ◽  
Elena Marangon ◽  
Mauro Buzzo ◽  
Giuseppe Toffoli ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Human Plasma ◽  
Anticancer Drug ◽  
Surface Enhanced Raman Spectroscopy ◽  
Label Free ◽  
Label Free Quantification ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Free Quantification

Label-free quantification of asymmetric cancer-cell filopodium activities in a multi-gradient chip

Lab on a Chip ◽  
2009 ◽  
Vol 9 (7) ◽  
pp. 884 ◽  
Author(s):  
Tsi-Hsuan Hsu ◽  
Meng-Hua Yen ◽  
Wei-Yu Liao ◽  
Ji-Yen Cheng ◽  
Chau-Hwang Lee
Keyword(s):  
Cancer Cell ◽  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification

scholarly journals Improved Intensity-Based Label-Free Quantification via Proximity-Based Intensity Normalization (PIN)

2014 ◽  
Vol 13 (3) ◽  
pp. 1281-1292 ◽  
Author(s):  
Susan K. Van Riper ◽  
Ebbing P. de Jong ◽  
LeeAnn Higgins ◽  
John V. Carlis ◽  
Timothy J. Griffin
Keyword(s):  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification ◽  
Intensity Normalization

scholarly journals Assessment of Label-Free Quantification in Discovery Proteomics and Impact of Technological Factors and Natural Variability of Protein Abundance

2017 ◽  
Vol 16 (4) ◽  
pp. 1410-1424 ◽  
Author(s):  
MHD Rami Al Shweiki ◽  
Susann Mönchgesang ◽  
Petra Majovsky ◽  
Domenika Thieme ◽  
Diana Trutschel ◽  
...  
Keyword(s):  
Natural Variability ◽  
Protein Abundance ◽  
Label Free ◽  
Technological Factors ◽  
Label Free Quantification ◽  
Free Quantification

scholarly journals StPeter: Seamless Label-Free Quantification with the Trans-Proteomic Pipeline

2018 ◽  
Vol 17 (3) ◽  
pp. 1314-1320 ◽  
Author(s):  
Michael R. Hoopmann ◽  
Jason M. Winget ◽  
Luis Mendoza ◽  
Robert L. Moritz
Keyword(s):  
Label Free ◽  
Label Free Quantification ◽  
Free Quantification
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