Imaging mass spectrometry to visualize biomolecule distributions in mouse brain tissue following hemispheric cortical spreading depression

2012 ◽  
Vol 75 (16) ◽  
pp. 5027-5035 ◽  
Author(s):  
Emrys A. Jones ◽  
Reinald Shyti ◽  
René J.M. van Zeijl ◽  
Sandra H. van Heiningen ◽  
Michel D. Ferrari ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Imaging Mass Spectrometry

Inside front cover: In situ detection of histone variants and modifications in mouse brain using imaging mass spectrometry

PROTEOMICS ◽  
2016 ◽  
Vol 16 (3) ◽  
pp. NA-NA
Author(s):  
Shibojyoti Lahiri ◽  
Na Sun ◽  
Victor Solis-Mezarino ◽  
Andreas Fedisch ◽  
Jovica Ninkovic ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry ◽  
Histone Variants ◽  
Front Cover ◽  
In Situ Detection

scholarly journals Three-dimensional visualization of protein expression in mouse brain structures using imaging mass spectrometry

2005 ◽  
Vol 16 (7) ◽  
pp. 1093-1099 ◽  
Author(s):  
Anna C. Crecelius ◽  
D. Shannon Cornett ◽  
Richard M. Caprioli ◽  
Betsy Williams ◽  
Benoit M. Dawant ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Expression ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry ◽  
Three Dimensional ◽  
Brain Structures

scholarly journals Mass spectrometry imaging of amino neurotransmitters: a comparison of derivatization methods and application in mouse brain tissue

Metabolomics ◽  
2016 ◽  
Vol 12 (2) ◽  
Author(s):  
Clara Esteve ◽  
Else A. Tolner ◽  
Reinald Shyti ◽  
Arn M. J. M. van den Maagdenberg ◽  
Liam A. McDonnell
Keyword(s):  
Mass Spectrometry ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Mass Spectrometry Imaging

scholarly journals ET-13 CONTROL OF ACTIVATED MICROGLIA THROUGH P2X4 RECEPTOR IN RADIATION BRAIN NECROSIS

2019 ◽  
Vol 1 (Supplement_2) ◽  
pp. ii10-ii10
Author(s):  
Kondo Natsuko ◽  
Sakurai Yoshinori ◽  
Takayuki Kajihara ◽  
Takushi Takada ◽  
Nobuhiko Takai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Right Hemisphere ◽  
Imaging Mass Spectrometry ◽  
Underlying Mechanism ◽  
Lipid Mediators ◽  
Tumor Patients ◽  
P2x4 Receptor ◽  
Activated Microglia ◽  
Brain Radiation

Abstract INTRODUCTION Brain radiation necrosis (RN) is severe adverse event after radiation therapy for brain tumor patients, especially in case of re-irradiation. Although corticosteroids or vitamin E, etc. are clinically used for RN, the effect is limited and underlying mechanism is to be cleared. Therefore, we established RN mouse model with irradiating right hemisphere of mouse brain using proton beam at dose of 60 Gy [Kondo et al., 2015]. In this study, we investigated change of phospholipids and lipid mediators after irradiation using this RN model in correlation with microglia activation. METHODS After irradiation, change of phospholipids and lipid mediators in mouse brain was investigated using imaging mass spectrometry and LC-MS. Immunohistochemistry on microglia and P2X4 receptor, a receptor for lysophosphatidylcholine (LPC) was performed. RESULTS In imaging mass spectrometry, 1 and 4 months after irradiation, phosphatidylcholine (PC): (16:0/20:4), (18:0/20:4) decreased in irradiated area compared non-irradiated area. On the other hand, LPC: (16:0) increased in irradiated area compared to non-irradiated area after 1 month and 4 months irradiation. PC (16:0/20:4) is a precursor of LPC (16:0) and arachidonic acid (20:4). By LC-MS, LPC was twice higher in irradiated area compared to non-irradiated, 6 months after irradiation. Microglia was highly activated in irradiated area compared to non-irradiated from 3 months after irradiation to 8 months and strongly co-expressed P2X4 receptor was confirmed in irradiated area after 6 months. Preliminary P2X4 receptor agonist administration test prolonged the RN to 12 months after irradiation. CONCLUSION In RN, LPC may continuously activated microglia through P2X4 receptor and cause chronic inflammation after irradiation. P2X4 agonist administration test including action resolution and immunohistochemistry is ongoing.

Direct profiling of myelinated and demyelinated regions in mouse brain by imaging mass spectrometry

2007 ◽  
Vol 260 (2-3) ◽  
pp. 185-194 ◽  
Author(s):  
Ruben Ceuppens ◽  
Debora Dumont ◽  
Leen Van Brussel ◽  
Babs Van de Plas ◽  
Ruth Daniels ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry

Validation and quantification of genomic 5-carboxylcytosine (5caC) in mouse brain tissue by liquid chromatography-tandem mass spectrometry

2016 ◽  
Vol 8 (29) ◽  
pp. 5812-5817 ◽  
Author(s):  
Lijian Zhang ◽  
Zhaojin Li ◽  
Guangzhao Chen ◽  
Qionglin Huang ◽  
Junjie Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Accurate Method ◽  
Tandem Mass ◽  
Validation And Quantification ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

A simple, sensitive and accurate method was developed for 5-carbocytosine detection by LC-MS/MS and was applied to mouse brain tissue.

scholarly journals Sarcoma Family Kinase-Dependent Pannexin-1 Activation after Cortical Spreading Depression Is Mediated by NR2A-Containing Receptors

2020 ◽  
Vol 21 (4) ◽  
pp. 1269 ◽  
Author(s):  
Fan Bu ◽  
Lingdi Nie ◽  
John P Quinn ◽  
Minyan Wang
Keyword(s):  
Mouse Brain ◽  
Protein Interactions ◽  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Regulatory Mechanism ◽  
Brain Slices ◽  
Pannexin 1 ◽  
Migraine Headaches ◽  
Aspartate Receptor ◽  
Channel Opening

Cortical spreading depression (CSD) is a propagating wave of depolarization followed by depression of cortical activity. CSD triggers neuroinflammation via the pannexin-1 (Panx1) channel opening, which may eventually cause migraine headaches. However, the regulatory mechanism of Panx1 is unknown. This study investigates whether sarcoma family kinases (SFK) are involved in transmitting CSD-induced Panx1 activation, which is mediated by the NR2A-containing N-methyl-D-aspartate receptor. CSD was induced by topical application of K+ to cerebral cortices of rats and mouse brain slices. SFK inhibitor, PP2, or NR2A–receptor antagonist, NVP–AAM077, was perfused into contralateral cerebral ventricles (i.c.v.) of rats prior to CSD induction. Co-immunoprecipitation and Western blot were used for detecting protein interactions, and histofluorescence for addressing Panx1 activation. The results demonstrated that PP2 attenuated CSD-induced Panx1 activation in rat ipsilateral cortices. Cortical susceptibility to CSD was reduced by PP2 in rats and by TAT-Panx308 that disrupts SFK–Panx1 interaction in mouse brain slices. Furthermore, CSD promoted activated SFK coupling with Panx1 in rat ipsilateral cortices. Moreover, inhibition of NR2A by NVP–AAM077 reduced elevation of ipsilateral SFK–Panx1 interaction, Panx1 activation induced by CSD and cortical susceptibility to CSD in rats. These data suggest NR2A-regulated, SFK-dependent Panx1 activity plays an important role in migraine aura pathogenesis.

scholarly journals Visualising mouse neuroanatomy and function by metal distribution using laser ablation-inductively coupled plasma-mass spectrometry imaging

2015 ◽  
Vol 6 (10) ◽  
pp. 5383-5393 ◽  
Author(s):  
Bence Paul ◽  
Dominic J. Hare ◽  
David P. Bishop ◽  
Chad Paton ◽  
Van Tran Nguyen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Mouse Brain ◽  
Inductively Coupled Plasma ◽  
Mass Spectrometry Imaging ◽  
Imaging Mass Spectrometry ◽  
Metal Distribution ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
And Function

Studying the neuroanatomy of the mouse brain using imaging mass spectrometry and chemometric analysis.

Sign in / Sign up

Export Citation Format

Share Document