scholarly journals Three-dimensional visualization of protein expression in mouse brain structures using imaging mass spectrometry

2005 ◽  
Vol 16 (7) ◽  
pp. 1093-1099 ◽  
Author(s):  
Anna C. Crecelius ◽  
D. Shannon Cornett ◽  
Richard M. Caprioli ◽  
Betsy Williams ◽  
Benoit M. Dawant ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Expression ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry ◽  
Three Dimensional ◽  
Brain Structures

Inside front cover: In situ detection of histone variants and modifications in mouse brain using imaging mass spectrometry

PROTEOMICS ◽  
2016 ◽  
Vol 16 (3) ◽  
pp. NA-NA
Author(s):  
Shibojyoti Lahiri ◽  
Na Sun ◽  
Victor Solis-Mezarino ◽  
Andreas Fedisch ◽  
Jovica Ninkovic ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry ◽  
Histone Variants ◽  
Front Cover ◽  
In Situ Detection

scholarly journals Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging

2012 ◽  
Vol 44 (15) ◽  
pp. 778-785 ◽  
Author(s):  
Jacqueline A. Gleave ◽  
Michael D. Wong ◽  
Jun Dazai ◽  
Maliha Altaf ◽  
R. Mark Henkelman ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Mouse Brain ◽  
Imaging Techniques ◽  
Three Dimensional ◽  
Brain Structures ◽  
Small Scale ◽  
Specimen Preparation ◽  
Normal Brain ◽  
Autofluorescence Imaging ◽  
The Brain

The structural organization of the brain is important for normal brain function and is critical to understand in order to evaluate changes that occur during disease processes. Three-dimensional (3D) imaging of the mouse brain is necessary to appreciate the spatial context of structures within the brain. In addition, the small scale of many brain structures necessitates resolution at the ∼10 μm scale. 3D optical imaging techniques, such as optical projection tomography (OPT), have the ability to image intact large specimens (1 cm3) with ∼5 μm resolution. In this work we assessed the potential of autofluorescence optical imaging methods, and specifically OPT, for phenotyping the mouse brain. We found that both specimen size and fixation methods affected the quality of the OPT image. Based on these findings we developed a specimen preparation method to improve the images. Using this method we assessed the potential of optical imaging for phenotyping. Phenotypic differences between wild-type male and female mice were quantified using computer-automated methods. We found that optical imaging of the endogenous autofluorescence in the mouse brain allows for 3D characterization of neuroanatomy and detailed analysis of brain phenotypes. This will be a powerful tool for understanding mouse models of disease and development and is a technology that fits easily within the workflow of biology and neuroscience labs.

scholarly journals ET-13 CONTROL OF ACTIVATED MICROGLIA THROUGH P2X4 RECEPTOR IN RADIATION BRAIN NECROSIS

2019 ◽  
Vol 1 (Supplement_2) ◽  
pp. ii10-ii10
Author(s):  
Kondo Natsuko ◽  
Sakurai Yoshinori ◽  
Takayuki Kajihara ◽  
Takushi Takada ◽  
Nobuhiko Takai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Right Hemisphere ◽  
Imaging Mass Spectrometry ◽  
Underlying Mechanism ◽  
Lipid Mediators ◽  
Tumor Patients ◽  
P2x4 Receptor ◽  
Activated Microglia ◽  
Brain Radiation

Abstract INTRODUCTION Brain radiation necrosis (RN) is severe adverse event after radiation therapy for brain tumor patients, especially in case of re-irradiation. Although corticosteroids or vitamin E, etc. are clinically used for RN, the effect is limited and underlying mechanism is to be cleared. Therefore, we established RN mouse model with irradiating right hemisphere of mouse brain using proton beam at dose of 60 Gy [Kondo et al., 2015]. In this study, we investigated change of phospholipids and lipid mediators after irradiation using this RN model in correlation with microglia activation. METHODS After irradiation, change of phospholipids and lipid mediators in mouse brain was investigated using imaging mass spectrometry and LC-MS. Immunohistochemistry on microglia and P2X4 receptor, a receptor for lysophosphatidylcholine (LPC) was performed. RESULTS In imaging mass spectrometry, 1 and 4 months after irradiation, phosphatidylcholine (PC): (16:0/20:4), (18:0/20:4) decreased in irradiated area compared non-irradiated area. On the other hand, LPC: (16:0) increased in irradiated area compared to non-irradiated area after 1 month and 4 months irradiation. PC (16:0/20:4) is a precursor of LPC (16:0) and arachidonic acid (20:4). By LC-MS, LPC was twice higher in irradiated area compared to non-irradiated, 6 months after irradiation. Microglia was highly activated in irradiated area compared to non-irradiated from 3 months after irradiation to 8 months and strongly co-expressed P2X4 receptor was confirmed in irradiated area after 6 months. Preliminary P2X4 receptor agonist administration test prolonged the RN to 12 months after irradiation. CONCLUSION In RN, LPC may continuously activated microglia through P2X4 receptor and cause chronic inflammation after irradiation. P2X4 agonist administration test including action resolution and immunohistochemistry is ongoing.

Direct profiling of myelinated and demyelinated regions in mouse brain by imaging mass spectrometry

2007 ◽  
Vol 260 (2-3) ◽  
pp. 185-194 ◽  
Author(s):  
Ruben Ceuppens ◽  
Debora Dumont ◽  
Leen Van Brussel ◽  
Babs Van de Plas ◽  
Ruth Daniels ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry

Imaging mass spectrometry to visualize biomolecule distributions in mouse brain tissue following hemispheric cortical spreading depression

2012 ◽  
Vol 75 (16) ◽  
pp. 5027-5035 ◽  
Author(s):  
Emrys A. Jones ◽  
Reinald Shyti ◽  
René J.M. van Zeijl ◽  
Sandra H. van Heiningen ◽  
Michel D. Ferrari ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Imaging Mass Spectrometry
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