Proteomic analysis of Bacillus thuringiensis ΔphaC mutant BMB171/PHB−1 reveals that the PHB synthetic pathway warrants normal carbon metabolism

2012 ◽  
Vol 75 (17) ◽  
pp. 5176-5188 ◽  
Author(s):  
Deju Chen ◽  
Dong Xu ◽  
Mingshun Li ◽  
Jin He ◽  
Yuhua Gong ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Carbon Metabolism ◽  
Proteomic Analysis ◽  
Synthetic Pathway ◽  
Normal Carbon

scholarly journals iTRAQ-based proteomic analysis reveals the molecule mechanism of reducing higher alcohols in Chinese rice wine by nitrogen compensation

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Guidong Huang ◽  
Hong Ren ◽  
Ali Wang ◽  
Xinran Wan ◽  
Ziying Wu ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Carbon Metabolism ◽  
Proteomic Analysis ◽  
Metabolic Flux ◽  
Quantitative Polymerase Chain Reaction ◽  
Higher Alcohols ◽  
Rice Wine ◽  
Chinese Rice Wine ◽  
Higher Alcohol ◽  
Amino Acid Synthesis

Abstract Purpose Higher alcohol is a by-product of the fermentation of wine, and its content is one of the most important parameters that affect and are used to appraise the final quality of Chinese rice wine. Ammonium compensation is an efficient and convenient method to reduce the content of higher alcohols, but the molecule mechanism is poorly understood. Therefore, an iTRAQ-based proteomic analysis was designed to reveal the proteomic changes of Saccharomyces cerevisiae to elucidate the molecular mechanism of ammonium compensation in reducing the content of higher alcohols. Methods The iTRAQ proteomic analysis method was used to analyze a blank group and an experimental group with an exogenous addition of 200 mg/L (NH4)2HPO4 during inoculation. The extracted intracellular proteins were processed by liquid chromatography-mass spectrometry and identified using bioinformatics tools. Real-time quantitative polymerase chain reaction was used to verify the gene expression of differentially expressed proteins. Results About 4062 proteins, including 123 upregulated and 88 downregulated proteins, were identified by iTRAQ-based proteomic analysis. GO and KEGG analysis uncovered that significant proteins were concentrated during carbohydrate metabolism, such as carbon metabolism, glyoxylate, and dicarboxylate metabolism, pyruvate metabolism, and the nitrogen metabolism, such as amino acid synthesis and catabolism pathway. In accordance with the trend of differential protein regulation in the central carbon metabolism pathway and the analysis of carbon metabolic flux, a possible regulatory model was proposed and verified, in which ammonium compensation facilitated glucose consumption, regulated metabolic flow direction into tricarboxylic acid, and further led to a decrease in higher alcohols. The results of RT-qPCR confirmed the authenticity of the proteomic analysis results at the level of gene. Conclusion Ammonium assimilation promoted by ammonium compensation regulated the intracellular carbon metabolism of S. cerevisiae and affected the distribution of metabolic flux. The carbon flow that should have gone to the synthesis pathway of higher alcohols was reversed to the TCA cycle, thereby decreasing the content of higher alcohols. These findings may contribute to an improved understanding of the molecular mechanism for the decrease in higher alcohol content through ammonium compensation.

Proteomic Analysis and Promoter Modification of Bacillus thuringiensis to Improve Insecticidal Vip3A Protein Production

2021 ◽  
Author(s):  
Sumarin Soonsanga ◽  
Amporn Rungrod ◽  
Narumon Phaonakrop ◽  
Sittiruk Roytrakul ◽  
Boonhiang Promdonkoy
Keyword(s):  
Bacillus Thuringiensis ◽  
Proteomic Analysis ◽  
Protein Production

scholarly journals A mitochondrial GABA permease connects the GABA shunt and the TCA cycle, and is essential for normal carbon metabolism

2011 ◽  
Vol 67 (3) ◽  
pp. 485-498 ◽  
Author(s):  
Simon Michaeli ◽  
Aaron Fait ◽  
Kelly Lagor ◽  
Adriano Nunes-Nesi ◽  
Nicole Grillich ◽  
...  
Keyword(s):  
Carbon Metabolism ◽  
Tca Cycle ◽  
Gaba Shunt ◽  
The Tca Cycle ◽  
Normal Carbon

Characterization of new Bacillus thuringiensis strains from Iran, based on cytocidal and insecticidal activity, proteomic analysis and gene content

BioControl ◽  
2018 ◽  
Vol 63 (6) ◽  
pp. 807-818 ◽  
Author(s):  
Ayda Khorramnejad ◽  
Reza Talaei-Hassanloui ◽  
Vahid Hosseininaveh ◽  
Yolanda Bel ◽  
Baltasar Escriche
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity ◽  
Proteomic Analysis ◽  
Gene Content

Proteomic analysis reveals the strategies of Bacillus thuringiensis YBT-1520 for survival under long-term heat stress

PROTEOMICS ◽  
2011 ◽  
Vol 11 (13) ◽  
pp. 2580-2591 ◽  
Author(s):  
Dandan Wu ◽  
Jin He ◽  
Yuhua Gong ◽  
Deju Chen ◽  
Xiaoli Zhu ◽  
...  
Keyword(s):  
Heat Stress ◽  
Bacillus Thuringiensis ◽  
Proteomic Analysis

scholarly journals Comparative proteomic analysis of thick-walled ray formation of Haloxylon ammodendron in the Gurbantunggut Desert, China

2020 ◽  
Author(s):  
Chaobin Zhou ◽  
Junjie Ding ◽  
Xiaojing Hu ◽  
Wei Gong
Keyword(s):  
Cell Wall ◽  
Wall Thickness ◽  
Carbon Metabolism ◽  
Proteomic Analysis ◽  
Metabolic Pathways ◽  
Cell Wall Biosynthesis ◽  
Haloxylon Ammodendron ◽  
Ray Cells ◽  
Phenylpropanoid Biosynthesis ◽  
Ray Cell

Abstract Background The thick-walled ray cells have been reported in Haloxylon ammodendron for the first time. This study measured the wall thickness of ray cells and performed a proteomic analysis of ray cell wall formation in the xylem of H. ammodendron using isobaric tags for relative and absolute quantitation. Results The wall thickness of ray cells in Jinghe (2.85 ± 0.42 µm) was significantly lower than that in Shihezi (3.08 ± 0.44 µm) (P < 0.01). In Shihezi, which has a thicker wall of ray cells than that in Jinghe, 795 differentially expressed proteins were upregulated. Phenylpropanoid biosynthesis, photosynthesis, glycolysis/gluconeogenesis, carbon metabolism, starch and sucrose metabolism, metabolic pathways, etc. promote ray cell wall biosynthesis of the xylem of H. ammodendron by providing substrates or energy. During the process of cell wall biosynthesis in the xylem of H. ammodendron, the nonspecific lipid-transfer protein and beta expansin EXPB2.1 (Mirabilis jalapa] first loosens the cell wall, followed by extension and expansion, and the xyloglucan endotransglycosylase/hydrolase 1 cleaves and links the xyloglucan chains. Then, photosystem I P700 apoprotein A1, reversibly glycosylated polypeptide 1 and GDP-mannose-3′, 5′-epimerase, etc., are involved in cellulose, hemicellulose and pectin biosynthesis of the cell wall by providing components or energy. Finally, the proteins in phenylpropanoid biosynthesis promote the lignification of the ray cell wall and complete the biosynthetic process of the cell wall. Conclusions Phenylpropanoid biosynthesis, photosynthesis, glycolysis/gluconeogenesis, carbon metabolism, starch and sucrose metabolism, metabolic pathways, etc. promote ray cell wall biosynthesis of the xylem of H. ammodendron by providing substrates or energy. The results are important for improving the wood mechanical properties of timber plantations.

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