Proteomic analysis reveals the strategies of Bacillus thuringiensis YBT-1520 for survival under long-term heat stress

PROTEOMICS ◽  
2011 ◽  
Vol 11 (13) ◽  
pp. 2580-2591 ◽  
Author(s):  
Dandan Wu ◽  
Jin He ◽  
Yuhua Gong ◽  
Deju Chen ◽  
Xiaoli Zhu ◽  
...  
Keyword(s):  
Heat Stress ◽  
Bacillus Thuringiensis ◽  
Proteomic Analysis

scholarly journals Linking population size structure, heat stress and bleaching responses in a subtropical endemic coral

Coral Reefs ◽  
2021 ◽  
Author(s):  
Liam Lachs ◽  
Brigitte Sommer ◽  
James Cant ◽  
Jessica M. Hodge ◽  
Hamish A. Malcolm ◽  
...  
Keyword(s):  
Heat Stress ◽  
Size Structure ◽  
Temporal Trends ◽  
Early Recovery ◽  
Size Frequency Distribution ◽  
Frequency Distributions ◽  
Community Organisation ◽  
Size Frequency ◽  
Eastern Australia

AbstractAnthropocene coral reefs are faced with increasingly severe marine heatwaves and mass coral bleaching mortality events. The ensuing demographic changes to coral assemblages can have long-term impacts on reef community organisation. Thus, understanding the dynamics of subtropical scleractinian coral populations is essential to predict their recovery or extinction post-disturbance. Here we present a 10-yr demographic assessment of a subtropical endemic coral, Pocillopora aliciae (Schmidt-Roach et al. in Zootaxa 3626:576–582, 2013) from the Solitary Islands Marine Park, eastern Australia, paired with long-term temperature records. These coral populations are regularly affected by storms, undergo seasonal thermal variability, and are increasingly impacted by severe marine heatwaves. We examined the demographic processes governing the persistence of these populations using inference from size-frequency distributions based on log-transformed planar area measurements of 7196 coral colonies. Specifically, the size-frequency distribution mean, coefficient of variation, skewness, kurtosis, and coral density were applied to describe population dynamics. Generalised Linear Mixed Effects Models were used to determine temporal trends and test demographic responses to heat stress. Temporal variation in size-frequency distributions revealed various population processes, from recruitment pulses and cohort growth, to bleaching impacts and temperature dependencies. Sporadic recruitment pulses likely support population persistence, illustrated in 2010 by strong positively skewed size-frequency distributions and the highest density of juvenile corals measured during the study. Increasing mean colony size over the following 6 yr indicates further cohort growth of these recruits. Severe heat stress in 2016 resulted in mass bleaching mortality and a 51% decline in coral density. Moderate heat stress in the following years was associated with suppressed P. aliciae recruitment and a lack of early recovery, marked by an exponential decrease of juvenile density (i.e. recruitment) with increasing heat stress. Here, population reliance on sporadic recruitment and susceptibility to heat stress underpin the vulnerability of subtropical coral assemblages to climate change.

Short- and long-term dynamics of the physiological and behavioral response to heat stress and thymol supplementation in Japanese quail

2021 ◽  
pp. 102876
Author(s):  
Maria Emilia Fernandez ◽  
Maria Carla Labaque ◽  
Gabriel Orso ◽  
Raúl Hector Marin ◽  
Jackelyn Melissa Kembro
Keyword(s):  
Heat Stress ◽  
Japanese Quail ◽  
Behavioral Response ◽  
Short And Long Term

An ER–Golgi Tethering Factor SLOH4/MIP3 Is Involved in Long-term Heat Tolerance of Arabidopsis

2020 ◽  
Author(s):  
Kazuho Isono ◽  
Ryo Tsukimoto ◽  
Satoshi Iuchi ◽  
Akihisa Shinozawa ◽  
Izumi Yotsui ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Tolerance ◽  
Secretory Proteins ◽  
Wild Type ◽  
Additional Function ◽  
Transcript Levels ◽  
Unfolded Protein ◽  
In The Wild ◽  
Protein Response

Abstract Plants are often exposed not only to short-term (S-) heat stress but also to diurnal long-term (L-) heat stress over several consecutive days. To reveal the mechanisms underlying L-heat stress tolerance, we here used a forward genetic screening for sensitive to long-term heat (sloh) mutants and isolated sloh4. The mutant was hypersensitive to L- but not S-heat stress. The causal gene of sloh4 was identical to MIP3 encoding a member of the MAIGO2 (MAG2) tethering complex, which is composed of the MAG2, MIP1, MIP2, and MIP3 subunits and is localized at the endoplasmic reticulum (ER) membrane. Although sloh4/mip3 was hypersensitive to L-heat stress, the sensitivity of the mag2-3 and mip1–1 mutants was similar to that of the wild type. Under L-heat stress, the ER stress and the following unfolded protein response (UPR) were more pronounced in sloh4 than in the wild type. Transcript levels of bZIP60-regulated UPR genes were strongly increased in sloh4 under L-heat stress. Two processes known to be mediated by INOSITOL REQUIRING ENZYME1 (IRE1)—accumulation of the spliced bZIP60 transcript and a decrease in the transcript levels of PR4 and PRX34, encoding secretory proteins—were observed in sloh4 in response to L-heat stress. These findings suggest that misfolded proteins generated in sloh4 under L-heat stress may be recognized by IRE1 but not bZIP28, resulting in initiation of the UPR via activated bZIP60. Therefore, it would be possible that only MIP3 in MAG2 complex has an additional function in L-heat tolerance, which is not related to the ER–Golgi vesicle tethering.

Immunolocalization of antioxidant enzymes in testis of rams submitted to long-term heat stress

Zygote ◽  
2019 ◽  
Vol 27 (6) ◽  
pp. 432-435
Author(s):  
Thais Rose dos Santos Hamilton ◽  
Gabriela Esteves Duarte ◽  
José Antonio Visintin ◽  
Mayra Elena Ortiz D’Ávila Assumpção
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Heat Stress ◽  
Glutathione Peroxidase ◽  
Cell Types ◽  
Treated Group ◽  
Oxidative Balance ◽  
Testicular Cells

SummaryLong-term heat stress (HS) induced by testicular insulation generates oxidative stress (OS) on the testicular environment; consequently activating antioxidant enzymes such as superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx). The aim of this work was to immunolocalize antioxidant enzymes present in different cells within the seminiferous tubule when rams were submitted to HS. Rams were divided into control (n = 6) and treated group (n = 6), comprising rams subjected to testicular insulation for 240 h. After the testicular insulation period, rams were subjected to orchiectomy. Testicular fragments were submitted to immunohistochemistry for staining against SOD, GR and GPx enzymes. We observed immunolocalization of GPx in more cell types of the testis after HS and when compared with other enzymes. In conclusion, GPx is the main antioxidant enzyme identified in testicular cells in an attempt to maintain oxidative balance when HS occurs.

scholarly journals Proteomic analysis of hippocampus in mice following long-term exposure to low levels of copper

2016 ◽  
Vol 5 (4) ◽  
pp. 1130-1139 ◽  
Author(s):  
Qian Sun ◽  
Ming Ying ◽  
Quan Ma ◽  
Zhijun Huang ◽  
Liangyu Zou ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Toxic Effects ◽  
Copper Exposure ◽  
Low Levels

Recent studies suggest that copper exposure, even at very low levels, can produce significant toxic effects on the brains of mice.

Proteomic analysis of long-term salinity stress-responsive proteins in Thellungiella halophila leaves

2008 ◽  
Vol 53 (22) ◽  
pp. 3530-3537 ◽  
Author(s):  
Fei Gao ◽  
YiJun Zhou ◽  
LingYun Huang ◽  
DaCheng He ◽  
GenFa Zhang
Keyword(s):  
Salinity Stress ◽  
Proteomic Analysis ◽  
Thellungiella Halophila

Characterization and Proteomic Analysis ofGeobacter sulfurreducensPCA under Long-Term Electron-Donor Starvation

2015 ◽  
pp. 00-00 ◽  
Author(s):  
Reema Bansal ◽  
Laura J. Liermann ◽  
Bruce A. Stanley ◽  
Susan L. Brantley ◽  
Ming Tien
Keyword(s):  
Electron Donor ◽  
Proteomic Analysis
Sign in / Sign up

Export Citation Format

Share Document