scholarly journals Proteomic analysis reveals cellular pathways regulating carbohydrate metabolism that are modulated in primary human skeletal muscle culture due to treatment with bioactives from Artemisia dracunculus L.

2012 ◽  
Vol 75 (11) ◽  
pp. 3199-3210 ◽  
Author(s):  
Peter Scherp ◽  
Nagireddy Putluri ◽  
Gary J. LeBlanc ◽  
Zhong Q. Wang ◽  
Xian H. Zhang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Carbohydrate Metabolism ◽  
Proteomic Analysis ◽  
Human Skeletal Muscle ◽  
Artemisia Dracunculus ◽  
Muscle Culture ◽  
Cellular Pathways

Regulation of insulin action by an extract of Artemisia dracunculus L. in primary human skeletal muscle culture: A proteomics approach

2010 ◽  
Vol 24 (9) ◽  
pp. 1278-1284 ◽  
Author(s):  
Indu Kheterpal ◽  
Liana Coleman ◽  
Ginger Ku ◽  
Zhong Q. Wang ◽  
David Ribnicky ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Action ◽  
Human Skeletal Muscle ◽  
Artemisia Dracunculus ◽  
Muscle Culture ◽  
Proteomics Approach

scholarly journals Bioactives of Artemisia dracunculus L enhance cellular insulin signaling in primary human skeletal muscle culture

Metabolism ◽  
2008 ◽  
Vol 57 ◽  
pp. S58-S64 ◽  
Author(s):  
Zhong Q. Wang ◽  
David Ribnicky ◽  
Xian H. Zhang ◽  
Ilya Raskin ◽  
Yongmei Yu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Signaling ◽  
Human Skeletal Muscle ◽  
Artemisia Dracunculus ◽  
Muscle Culture

Neuromuscular electrical stimulation training induces atypical adaptations of the human skeletal muscle phenotype: a functional and proteomic analysis

2011 ◽  
Vol 110 (2) ◽  
pp. 433-450 ◽  
Author(s):  
Julien Gondin ◽  
Lorenza Brocca ◽  
Elena Bellinzona ◽  
Giuseppe D'Antona ◽  
Nicola A. Maffiuletti ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Proteomic Analysis ◽  
Metabolic Profile ◽  
Human Skeletal Muscle ◽  
Protein Pattern ◽  
Neuromuscular Electrical Stimulation ◽  
Vastus Lateralis Muscle ◽  
Before And After ◽  
Antioxidant Defense Systems

The aim of the present study was to define the chronic effects of neuromuscular electrical stimulation (NMES) on the neuromuscular properties of human skeletal muscle. Eight young healthy male subjects were subjected to 25 sessions of isometric NMES of the quadriceps muscle over an 8-wk period. Needle biopsies were taken from the vastus lateralis muscle before and after training. The training status, myosin heavy chain (MHC) isoform distribution, and global protein pattern, as assessed by proteomic analysis, widely varied among subjects at baseline and prompted the identification of two subgroups: an “active” (ACT) group, which performed regular exercise and had a slower MHC profile, and a sedentary (SED) group, which did not perform any exercise and had a faster MHC profile. Maximum voluntary force and neural activation significantly increased after NMES in both groups (+∼30% and +∼10%, respectively). Both type 1 and 2 fibers showed significant muscle hypertrophy. After NMES, both groups showed a significant shift from MHC-2X toward MHC-2A and MHC-1, i.e., a fast-to-slow transition. Proteomic maps showing ∼500 spots were obtained before and after training in both groups. Differentially expressed proteins were identified and grouped into functional categories. The most relevant changes regarded 1) myofibrillar proteins, whose changes were consistent with a fast-to-slow phenotype shift and with a strengthening of the cytoskeleton; 2) energy production systems, whose changes indicated a glycolytic-to-oxidative shift in the metabolic profile; and 3) antioxidant defense systems, whose changes indicated an enhancement of intracellular defenses against reactive oxygen species. The adaptations in the protein pattern of the ACT and SED groups were different but were, in both groups, typical of both resistance (i.e., strength gains and hypertrophy) and endurance (i.e., a fast-to-slow shift in MHC and metabolic profile) training. These training-induced adaptations can be ascribed to the peculiar motor unit recruitment pattern associated with NMES.

Proteomic analysis of human skeletal muscle (m. vastus lateralis) proteins: Identification of 89 gene expression products

2009 ◽  
Vol 74 (11) ◽  
pp. 1239-1252 ◽  
Author(s):  
M. A. Kovalyova ◽  
L. I. Kovalyov ◽  
I. Yu. Toropygin ◽  
S. V. Shigeev ◽  
A. V. Ivanov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Proteomic Analysis ◽  
Human Skeletal Muscle ◽  
Vastus Lateralis

scholarly journals Artemisia dracunculusL. extract ameliorates insulin sensitivity by attenuating inflammatory signalling in human skeletal muscle culture

2014 ◽  
Vol 16 (8) ◽  
pp. 728-738 ◽  
Author(s):  
B. Vandanmagsar ◽  
K. R. Haynie ◽  
S. E. Wicks ◽  
E. M. Bermudez ◽  
T. M. Mendoza ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Sensitivity ◽  
Human Skeletal Muscle ◽  
Muscle Culture ◽  
Inflammatory Signalling
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