scholarly journals A novel luminol-based chemiluminescence method for the determination of amikacin sulfate in serum by using trivalent copper-periodate complex

2013 ◽  
Vol 3 (5) ◽  
pp. 360-366 ◽  
Author(s):  
Yu-Fei Hu ◽  
Gong-Ke Li ◽  
Zhu-Jun Zhang
Keyword(s):  
Chemiluminescence Method ◽  
Amikacin Sulfate

A chemiluminescence method for the determination of verapamil hydrochloride in pharmaceutical and environmental samples based on an Fe3O4 nanoparticles enhanced NaHCO3–H2O2 system

2017 ◽  
Vol 9 (47) ◽  
pp. 6705-6712 ◽  
Author(s):  
Mortaza Iranifam ◽  
Nasim Rahmati Hendekhale
Keyword(s):  
Fe3o4 Nanoparticles ◽  
Environmental Samples ◽  
Chemiluminescence Method ◽  
Verapamil Hydrochloride ◽  
Enhanced Chemiluminescence ◽  
System P ◽  
H2o2 System

In this work, an Fe3O4 nanoparticles (NPs)-enhanced chemiluminescence (CL) method was developed for the determination of verapamil hydrochloride (VRP).

scholarly journals Determination of phenobarbital in human urine and serum usingflow injection chemiluminescence

2012 ◽  
Vol 58 (1) ◽  
pp. 88-94
Author(s):  
K. Li ◽  
L.Z. Nu ◽  
K.L. Khe ◽  
K.H. Song
Keyword(s):  
Human Urine ◽  
Pharmaceutical Preparations ◽  
Injection System ◽  
Chemiluminescence Method ◽  
Chemiluminescence Intensity ◽  
Complete Determination ◽  
Flow Injection System ◽  
Human Urine And Serum ◽  
Urine And Serum

A sensitive chemiluminescence method, based on the enhancive effect of phenobarbital on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of phenobarbital. The chemiluminescence intensity responded to the concentration of phenobarbital linearly ranging from 0.05 to 10 ng⋅ml-1 with the detection limit of 0.02 ng⋅ml-1 (3σ). At a flow rate of 2.0 ml⋅min-1, a complete determination of phenobarbital, including sampling and washing, could be accomplished in 0.5 min, offering the sampling efficiency of 120 h-1 accordingly. The method was applied successfully in an assay of PB for pharmaceutical preparations, human urine and serum without any pretreatment with recovery from 95.7 to 106.7% and RSDs of less than 3.0%.

Determination of trivalent europium using flow injection chemiluminescence method

2004 ◽  
Vol 506 (1) ◽  
pp. 25-29 ◽  
Author(s):  
Shilv Chen ◽  
Huichun Zhao ◽  
Xiaoli Wang ◽  
Xia Li ◽  
Linpei Jin
Keyword(s):  
Flow Injection ◽  
Chemiluminescence Method ◽  
Trivalent Europium ◽  
Flow Injection Chemiluminescence

scholarly journals Effect of anti-thyroid peroxidase (TPO) antibodies on TPO activity measured by chemiluminescence assay

1997 ◽  
Vol 43 (8) ◽  
pp. 1392-1396 ◽  
Author(s):  
V Kaczur ◽  
Gy Vereb ◽  
I Molnár ◽  
G Krajczár ◽  
E Kiss ◽  
...  
Keyword(s):  
Western Blotting ◽  
Competitive Inhibition ◽  
High Sensitivity ◽  
Inhibitory Effect ◽  
Thyroid Peroxidase ◽  
Chemiluminescence Detection ◽  
Chemiluminescence Method ◽  
Blotting Technique ◽  
Hypothyroid Patients

Abstract A chemiluminescence method was developed to measure thyroid peroxidase (TPO) activity and the inhibitory effect of anti-TPO antibodies in purified porcine TPO. The TPO preparation was characterized kinetically and controlled by Western-blotting technique. The chemiluminescence method proved to be reproducible and much more sensitive than the widely used guaiacol method, being able to detect TPO concentrations of 2.21 × 10−5 g/L vs 6.63 × 10−2g/L with the latter. Otherwise, the determinations with the two methods correlated well (r = 0.76). Investigating the effect of IgGs from 23 hypothyroid patients on measured TPO activity, we detected inhibition in 19 cases with the chemiluminescence technique (15 with the guaiacol method). Anti-TPO antibodies showed competitive inhibition of TPO activity with respect to the substrate guaiacol. In both systems, the inhibition is present in the IgG F(ab′)2 fragment. We conclude that the high sensitivity of chemiluminescence detection allows routine determination of the inhibition of TPO activity by anti-TPO antibodies.

Determination of indomethacin in urine using molecule imprinting-chemiluminescence method

Talanta ◽  
2005 ◽  
Vol 66 (3) ◽  
pp. 728-733 ◽  
Author(s):  
F NIE ◽  
J LU ◽  
Y HE ◽  
J DU
Keyword(s):  
Chemiluminescence Method

Determination of norfloxacin in pharmaceuticals, human serum, and urine using a luminol—dissolved oxygen chemiluminescence system

2007 ◽  
Vol 61 (5) ◽  
Author(s):  
X. Shao ◽  
H. Liu ◽  
X. Gao ◽  
W. Chen ◽  
Z. Song
Keyword(s):  
Dissolved Oxygen ◽  
Calibration Graph ◽  
Injection System ◽  
Chemiluminescence Method ◽  
Relative Standard ◽  
Flow Injection System ◽  
Human Urine And Serum ◽  
Urine And Serum ◽  
Mechanism Of The Reaction

AbstractA sensitive chemiluminescence method, based on the enhancive effect of norfloxacin on the reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of norfloxacin. The increment of the chemiluminiscence intensity was proportional to the concentration of norfloxacin, giving a calibration graph linear over the concentration from 0.4 ng mL−1 to 400.0 ng mL−1 (r 2 = 0.9988) with the detection limit of 0.1 ng mL−1 (3 × σ noise). At the flow rate of 2.0 mL min−1, a complete determination of norfloxacin, including sampling and washing, could be accomplished in 30 s with the relative standard deviation lower than 3.0 %. The proposed method was applied successfully to determine norfloxacin in pharmaceuticals, human urine, and serum. Possible mechanism of the reaction was also discussed.

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