Development and validation of a UHPLC–MS/MS assay for colistin methanesulphonate (CMS) and colistin in human plasma and urine using weak-cation exchange solid-phase extraction

2016 ◽  
Vol 124 ◽  
pp. 303-308 ◽  
Author(s):  
Miao Zhao ◽  
Xiao-Jie Wu ◽  
Ya-Xin Fan ◽  
Bei-Ning Guo ◽  
Jing Zhang
Keyword(s):  
Solid Phase Extraction ◽  
Cation Exchange ◽  
Human Plasma ◽  
Solid Phase ◽  
Phase Extraction ◽  
Human Plasma And Urine ◽  
Weak Cation Exchange ◽  
Development And Validation

scholarly journals Preparation and utilization of a molecularly imprinted polymer for solid phase extraction of tramadol

2010 ◽  
Vol 8 (4) ◽  
pp. 861-869 ◽  
Author(s):  
Saman Azodi-Deilami ◽  
Majid Abdouss ◽  
Seyed Hasani
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Molecularly Imprinted Polymer ◽  
Solid Phase ◽  
Extraction Procedure ◽  
Molecular Structures ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Human Plasma And Urine

AbstractIn this paper, a highly selective molecularly imprinted polymer (MIP) for tramadol hydrochloride, a drug used to treat moderate to severe pain, was prepared and its use as solid-phase extraction (SPE) sorbent was demonstrated. The molecularly imprinted solid-phase extraction procedure followed by high performance liquid chromatography with ultraviolet detector (MISPE-HPLC) was developed for selective extraction and determination of tramadol in human plasma and urine. The optimal conditions for molecularly imprinted solid-phase extraction (MISPE) consisted of conditioning with 1 mL methanol and 1 mL of deionized water at neutral pH, loading of tramadol sample (50 µg L−1) at pH 7.5, washing using 1 mL acetone and elution with 3 × 1 mL of 10% (v/v) acetic acid in methanol. The MIP selectivity was evaluated by checking several substances with similar molecular structures to that of tramadol. Results from the HPLC analyses showed that the calibration curve of tramadol (using MIP from human plasma and urine) is linear in the ranges of 6–100 and 3–120 µg L−1 with good precisions (1.9% and 2.9% for 5.0 µg L−1), respectively. The recoveries for plasma and urine samples were higher than 81%.

Magnetic solid-phase extraction based on a trimethylstearylammonium bromide coated Fe3O4/SiO2 composite for determination of adriamycin hydrochloride in human plasma and urine by HPLC-FLD

2014 ◽  
Vol 6 (17) ◽  
pp. 6736-6744 ◽  
Author(s):  
Nengxuan Ma ◽  
Lin Zhang ◽  
Ruijin Li ◽  
Yehong Zhou ◽  
Zongwei Cai ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Solid Phase ◽  
Magnetic Solid Phase Extraction ◽  
Phase Extraction ◽  
Human Plasma And Urine ◽  
Magnetic Solid

A novel TSAB coated Fe3O4/SiO2 MNP was fabricated and used as the adsorbent for magnetic solid-phase extraction of ADR with HPLC-FLD.

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