New trends in reversed-phase liquid chromatographic separations of therapeutic peptides and proteins: Theory and applications

2012 ◽  
Vol 69 ◽  
pp. 9-27 ◽  
Author(s):  
Szabolcs Fekete ◽  
Jean-Luc Veuthey ◽  
Davy Guillarme
Keyword(s):  
Reversed Phase ◽  
Chromatographic Separations ◽  
Therapeutic Peptides ◽  
Phase Liquid ◽  
Liquid Chromatographic

SIMULTANEOUS DETERMINATION OF DOMPERIDONE AND PARABEN PRESERVATIVE IN ORAL FORMULATION: REVERSED-PHASE LIQUID CHROMATOGRAPHIC METHOD

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (08) ◽  
pp. 81-83
Author(s):  
A Patel ◽  
S. D Firke ◽  
R. R. Patil ◽  
M. G. Kalaskar ◽  
S. B. Bari ◽  
...  
Keyword(s):  
Sodium Benzoate ◽  
Gradient Elution ◽  
Oral Formulation ◽  
Reversed Phase ◽  
Hplc Method ◽  
Oral Solution ◽  
Chromatographic Separations ◽  
Phase Liquid ◽  
Liquid Chromatographic

A novel RP-HPLC method was developed and validated for the determination of compounds in an oral solution. The method describes the determination of domperidone along with sodium methylparaben, sodium propylparaben and sodium benzoate in liquid oral formulation. The chromatographic separations were performed using BDS Hypersil 5 μm C18 column and gradient elution (solvent A: phosphate buffer, pH 3.5 and solvent B: methanol) keeping a flow rate of 1.5 mL/min. Detection was done at dual wavelength (232 nm for domperidone and sodium benzoate, and 257 nm for sodium methylparaben and sodium propylparaben). Analysis time was < 17 min. The retention times for domperidone, sodium benzoate, sodium methylparaben and sodium propylparaben were found to be 10.0, 6.5, 8.0, and 13.5 min, The calibration curves for domperidone, sodium benzoate, sodium methylparaben and sodium propylparaben were found to be linear in the range of 250-750, 50-150, 50-150, and 5-15 μg/mL, respectively.

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