A method for characterization and optimization of reversed-phase liquid chromatographic separations based on the retention behaviour in homologous series

1984 ◽  
Vol 19 (1) ◽  
pp. 101-112 ◽  
Author(s):  
P. Jandera
Keyword(s):  
Homologous Series ◽  
Reversed Phase ◽  
Chromatographic Separations ◽  
Retention Behaviour ◽  
Phase Liquid ◽  
Liquid Chromatographic

SIMULTANEOUS DETERMINATION OF DOMPERIDONE AND PARABEN PRESERVATIVE IN ORAL FORMULATION: REVERSED-PHASE LIQUID CHROMATOGRAPHIC METHOD

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (08) ◽  
pp. 81-83
Author(s):  
A Patel ◽  
S. D Firke ◽  
R. R. Patil ◽  
M. G. Kalaskar ◽  
S. B. Bari ◽  
...  
Keyword(s):  
Sodium Benzoate ◽  
Gradient Elution ◽  
Oral Formulation ◽  
Reversed Phase ◽  
Hplc Method ◽  
Oral Solution ◽  
Chromatographic Separations ◽  
Phase Liquid ◽  
Liquid Chromatographic

A novel RP-HPLC method was developed and validated for the determination of compounds in an oral solution. The method describes the determination of domperidone along with sodium methylparaben, sodium propylparaben and sodium benzoate in liquid oral formulation. The chromatographic separations were performed using BDS Hypersil 5 μm C18 column and gradient elution (solvent A: phosphate buffer, pH 3.5 and solvent B: methanol) keeping a flow rate of 1.5 mL/min. Detection was done at dual wavelength (232 nm for domperidone and sodium benzoate, and 257 nm for sodium methylparaben and sodium propylparaben). Analysis time was < 17 min. The retention times for domperidone, sodium benzoate, sodium methylparaben and sodium propylparaben were found to be 10.0, 6.5, 8.0, and 13.5 min, The calibration curves for domperidone, sodium benzoate, sodium methylparaben and sodium propylparaben were found to be linear in the range of 250-750, 50-150, 50-150, and 5-15 μg/mL, respectively.

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