Use of collision induced dissociation mass spectrometry as a rapid technique for the identification of pharmacologically active peptides in pharmacopoeial testing

2011 ◽  
Vol 55 (5) ◽  
pp. 957-963 ◽  
Author(s):  
Ulrike Holzgrabe ◽  
Cees-Jan Nap ◽  
Stefan Almeling
Keyword(s):  
Mass Spectrometry ◽  
Collision Induced Dissociation ◽  
Active Peptides ◽  
Rapid Technique ◽  
Pharmacologically Active

scholarly journals Diversity of Conopeptides and Conoenzymes from the Venom Duct of the Marine Cone Snail Conus bayani as Determined from Transcriptomic and Proteomic Analyses

Marine Drugs ◽  
2021 ◽  
Vol 19 (4) ◽  
pp. 202
Author(s):  
Rajesh Rajaian Pushpabai ◽  
Carlton Ranjith Wilson Alphonse ◽  
Rajasekar Mani ◽  
Deepak Arun Apte ◽  
Jayaseelan Benjamin Franklin
Keyword(s):  
Gene Family ◽  
Transcriptome Sequencing ◽  
Cone Snail ◽  
Active Peptides ◽  
Transcriptomic Data ◽  
Sequencing Technologies ◽  
Hunting Strategies ◽  
Divergent Gene ◽  
Pharmacologically Active ◽  
Highly Evolved

Marine cone snails are predatory gastropods characterized by a well-developed venom apparatus and highly evolved hunting strategies that utilize toxins to paralyze prey and defend against predators. The venom of each species of cone snail has a large number of pharmacologically active peptides known as conopeptides or conotoxins that are usually unique in each species. Nevertheless, venoms of only very few species have been characterized so far by transcriptomic approaches. In this study, we used transcriptome sequencing technologies and mass spectrometric methods to describe the diversity of venom components expressed by a worm-hunting species, Conus bayani. A total of 82 conotoxin sequences were retrieved from transcriptomic data that contain 54 validated conotoxin sequences clustered into 21 gene superfamilies including divergent gene family, 17 sequences clustered to 6 different conotoxin classes, and 11 conotoxins classified as unassigned gene family. Seven new conotoxin sequences showed unusual cysteine patterns. We were also able to identify 19 peptide sequences using mass spectrometry that completely overlapped with the conotoxin sequences obtained from transcriptome analysis. Importantly, herein we document the presence of 16 proteins that include five post-translational modifying enzymes obtained from transcriptomic data. Our results revealed diverse and novel conopeptides of an unexplored species that could be used extensively in biomedical research due to their therapeutic potentials.

Rapid Evaluation of Suitable Substrates with High Affinity to Artificial Caffeine Receptors by MS Based Techniques

2005 ◽  
Vol 60 (10) ◽  
pp. 1077-1082 ◽  
Author(s):  
Daniela Mirk ◽  
Heinrich Luftmann ◽  
Siegfried R. Waldvogel
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Collision Induced Dissociation ◽  
Rapid Evaluation ◽  
Tandem Mass ◽  
Binding Affinities ◽  
Electrospray Tandem Mass Spectrometry ◽  
Relative Stabilities ◽  
Receptor Complexes ◽  
High Affinity

A modification of our triphenylene ketal based receptor facilitates electrospray tandem mass spectrometry investigations. Binding affinities of eleven potential substrates, e.g. caffeine and other xanthine alkaloids, are probed in the gas phase with collision induced dissociation. The relative stabilities of the substrate-receptor complexes are rapidly determined and the findings are correlated with the corresponding results in solution.

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