In vitro free radical scavenging activities and effect of synthetic oligosaccharides on antioxidant enzymes and lipid peroxidation in aged mice

2007 ◽  
Vol 43 (1) ◽  
pp. 364-370 ◽  
Author(s):  
Xin-Ming Li ◽  
Yong-Hui Shi ◽  
Fang Wang ◽  
Hai-Song Wang ◽  
Guo-Wei Le
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Aged Mice ◽  
Synthetic Oligosaccharides ◽  
Free Radical Scavenging Activities

scholarly journals ANTIOXIDANT ACTIVITIES OF ROPINIROLE AND PRAMIPEXOLE NOVEL DRUGS USED IN TREATMENT OF PARKINSONISM: AN IN VITRO APPROACH

2016 ◽  
Vol 9 (9) ◽  
pp. 105 ◽  
Author(s):  
Preetha Selva ◽  
Srinivasan Vengadassalapathy
Keyword(s):  
Free Radical ◽  
Dopamine Agonist ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Novel Drugs ◽  
Dpph Method ◽  
Selective Dopamine ◽  
Free Radical Scavenging Activities

ABSTRACTObjective: The objective of this study is to evaluate and compare the free radical scavenging activities of selective dopamine agonist namely ropiniroleand pramipexole.Methods: The antioxidant activity of ropinirole and pramipexole at various concentrations was done by 1, 1-diphenyl-2picrylhydrazyl (DPPH) freeradical scavenging assay comparing it with ascorbic acid which was taken as standard.Results: The free radical scavenging property as measured by DPPH method showed that ropinirole and pramipexole have got a potent free radicalscavenging activity with that of ascorbic acid.Conclusion: Novel drugs such as pramipexole and ropinirole are promising molecules in the field of oxidative damage related neurodegenerativedisorders providing us an optimistic targeted approach toward neuroprotection.Keywords: Free radical scavenging, 1, 1-diphenyl-2picrylhydrazyl assay, Anti-Parkinson’s, Dopamine agonist.

Dithiols as more effective than monothiols in protecting biomacromolecules from free-radical-mediated damage: in vitro oxidative degradation of high-molar-mass hyaluronan

2014 ◽  
Vol 68 (10) ◽  
Author(s):  
Mária Baňasová ◽  
Katarína Valachová ◽  
Ivo Juránek ◽  
Ladislav Šoltés
Keyword(s):  
Free Radical ◽  
Molar Mass ◽  
Radical Scavenging ◽  
Oxidative Degradation ◽  
Free Radical Scavenging ◽  
Dpph Assay ◽  
High Molar Mass ◽  
High Concentrations ◽  
Free Radical Scavenging Activities

AbstractOxidative stress and the resulting damage to cellular and extracellular components has been observed in a variety of degenerative processes, including degenerative joint disorders, where high-molar-mass hyaluronan (HA) is often found to be massively degraded. The present study sought to test the hypothesis that dithiols are more effective in protecting biomacromolecules from free-radicalmediated damage than monothiols. The materials/thiols tested included bucillamine (BUC), dithioerythritol (DTE), dithiothreitol (DTT) and glutathione (GSH), as a reference, for their effectiveness in protecting HA from oxidative degradation induced in vitro. Since HA degradation results in a decrease in its dynamic viscosity, rotational viscometry was applied to follow HA oxidative degradation. The free-radical-scavenging activities of the thiols tested were determined by 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) assays. It was found that all the dithiols in the concentration range tested protected HA from the oxidative degradation. On the other hand, monothiol GSH exerted protection only at high concentrations (10 μmol L−1 and 100 μmol L−1) and 1 μmol L−1 of GSH even exhibited a prodegradative effect. The ABTS assay revealed free-radical scavenging activities in the following order: BUC, DTT, DTE, GSH, and that of the DPPH assay: BUC, DTE, DTT, GSH. In conclusion, it was demonstrated that dithiols may be more effective than monothiols in affording biomacromolecule protection from oxidative degradation.

scholarly journals In vitro inhibitory potential of avocado fruits, Persea americana (Lauraceae) against oxidation, inflammation and key enzymes linked to skin diseases

2021 ◽  
Vol 69 (2) ◽  
Author(s):  
Muhammed M. Hürkul ◽  
Sezen Yilmaz Sarialtin ◽  
Ayşegül Köroğlu ◽  
Tülay Çoban
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Skin Diseases ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Persea Americana ◽  
Methanol Extracts ◽  
Hexane Extracts ◽  
Anti Inflammatory ◽  
Free Radical Scavenging Activities

Avocado (Persea americana Mill.) is a member of Lauraceae with one-seeded berry fruit and cultivated in all tropical, subtropical regions in the world and in the Southern coast region of Turkey. Oxidative damage caused by UV can trigger inflammation, resulting in serious inflammatory skin diseases including eczema, seborrheic dermatitis, hyperpigmentation and ageing. Enzyme inhibitors involved in melanogenesis, such as tyrosinase, have been used recently for hyperpigmentation and skin diseases in cosmetic products. Objective: This study aimed to evaluate the antioxidant, anti-inflammatory, anti-tyrosinase activities and total polyphenolic contents of the different parts of P. americana fruit. Methods: The fruit was divided into exocarp, mesocarp, seed, and then methanol and n-hexane extracts were prepared. DPPH and ABTS free radical scavenging capacities and inhibitory potentials on lipid peroxidation were determined to investigate the antioxidant potentials of the extracts. Anti-inflammatory activities of the extracts were evaluated by measuring the stabilization level of the human red blood cell membrane. The tyrosinase inhibitory activities of the samples were determined using mushroom tyrosinase. Results: In general methanol extracts possessed remarkable higher DPPH free radical scavenging activities than n-hexane extracts. The highest activity was determined in methanol extracts of seed (4.17 ± 0.04 mg/mL) followed by exocarp (5.25 ± 0.05 mg/mL). Overall methanol extracts possessed higher ABTS free radical scavenging activities than n-hexane extracts. The greatest ABTS free radical scavenging activity was obtained in methanol extracts of seed (0.03 ± 0.01 mg/mL). In the anti-lipid peroxidation assay, the greatest activity was noticed in methanol extracts of seed (7.71 ± 0.36 µg/mL) followed by exocarp (12.12 ± 0.34 µg/mL), while all n-hexane extracts were inactive. Overall methanol extracts exhibited higher anti-inflammatory and antioxidant properties than n-hexane extracts. However, the maximum anti-tyrosinase activity was determined in n-hexane extracts of exocarp (0.40 ± 0.01 mg/mL) followed by seed (0.46 ± 0.01 mg/mL). Conclusions: These extracts are promising candidates for use as natural products-based antioxidant and anti-inflammatory properties in inflammation-related disease, and also anti-tyrosinase properties in dermatological applications.

scholarly journals Ethno-Pharmacological Survey, In Vitro Anti-Sickling and Free Radical Scavenging Activities of Carapa Procera DC. Stem Bark (Meliaceae)

2014 ◽  
Vol 03 (01) ◽  
pp. 1-10 ◽  
Author(s):  
Koto-te-Nyiwa Ngbolua ◽  
Tshitenge T. Bishola ◽  
Tshimankinda P. Mpiana ◽  
Virima Mudogo ◽  
Sha-Tshibey D. Tshibangu ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Free Radical Scavenging ◽  
Carapa Procera ◽  
Free Radical Scavenging Activities
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