The Use of Autogenous Bone and Alloplastic Materials Supplemented With Platelet-Rich Plasma in the Treatment of Critical Size Rat Calvarial Defects

2006 ◽  
Vol 64 (9) ◽  
pp. 67
Author(s):  
Samuel Tchon
Keyword(s):  
Critical Size ◽  
Platelet Rich Plasma ◽  
Autogenous Bone ◽  
Alloplastic Materials ◽  
Calvarial Defects

Influence of the proportion of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects

Bone ◽  
2009 ◽  
Vol 45 (2) ◽  
pp. 339-345 ◽  
Author(s):  
Maria Nagata ◽  
Michel Messora ◽  
Roberta Okamoto ◽  
Natália Campos ◽  
Natália Pola ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Healing ◽  
Critical Size ◽  
Platelet Rich Plasma ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Critical Size Defects

scholarly journals Exploring anorganic bovine bone granules as osteoblast carriers for bone bioengineering: a study in rat critical-size calvarial defects

2012 ◽  
Vol 23 (4) ◽  
pp. 315-321 ◽  
Author(s):  
Willian F. Zambuzzi ◽  
Gustavo V. O. Fernandes ◽  
Flávia G. Iano ◽  
Mileni da S. Fernandes ◽  
José Mauro Granjeiro ◽  
...  
Keyword(s):  
Critical Size ◽  
Blood Clot ◽  
Positive Control ◽  
Negative Control ◽  
Autogenous Bone ◽  
Control Group ◽  
Bovine Bone ◽  
Innovative Methods ◽  
Calvarial Defects ◽  
The Moment

It is known that current trends on bone bioengineering seek ideal scaffolds and explore innovative methods to restore tissue function. In this way, the objective of this study was to evaluate the behavior of anorganic bovine bone as osteoblast carrier in critical-size calvarial defects. MC3T3-E1 osteoblast cells (1x10(5) cells/well) were cultured on granules of anorganic bovine bone in 24-well plates and after 24 h these granules were implanted into rat critical-size calvarial defects (group Biomaterial + Cells). In addition, other groups were established with different fillings of the defect: Blood Clot (negative control); Autogenous Bone (positive control); Biomaterial (only granules) and Cells (only MC3T3-E1 cells). After 30 days, the animals were euthanized and the calvaria were technically processed in order to allow histological and morphometric analysis. It was possible to detect blood vessels, connective tissue and newly formed bone in all groups. Particularly in the Biomaterial + Cells group, it was possible to observe a profile of biological events between the positive control group (autogenous bone) and the group in which only anorganic bovine granules were implanted. Altogether, the results of the present study showed that granules of anorganic bovine bone can be used as carrier to osteoblasts and that adding growth factors at the moment of implantation should maximize these results.

Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects

2004 ◽  
Vol 15 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Joerg Wiltfang ◽  
Frank Rudolf Kloss ◽  
Peter Kessler ◽  
Emeka Nkenke ◽  
Stefan Schultze-mosgau ◽  
...  
Keyword(s):  
Bone Healing ◽  
Critical Size ◽  
Platelet Rich Plasma ◽  
Bone Substitutes ◽  
Autogenous Bone ◽  
Critical Size Defects

Effect of platelet-rich plasma on bone healing of autogenous bone grafts in critical-size defects

2009 ◽  
Vol 36 (9) ◽  
pp. 775-783 ◽  
Author(s):  
Maria J. H. Nagata ◽  
Luiz. G. N. Melo ◽  
Michel R. Messora ◽  
Suely R. M. Bomfim ◽  
Stephen E. Fucini ◽  
...  
Keyword(s):  
Bone Healing ◽  
Critical Size ◽  
Platelet Rich Plasma ◽  
Bone Grafts ◽  
Autogenous Bone ◽  
Critical Size Defects ◽  
Autogenous Bone Grafts

Histomorphometric Analysis of Bone Repair in Critical Size Defect in Rats Calvaria Treated with Hydroxyapatite and Zinc-Containing Hydroxyapatite 5%

2008 ◽  
Vol 396-398 ◽  
pp. 15-18 ◽  
Author(s):  
G.V.O. Fernandes ◽  
Monica Calasans-Maia ◽  
F.F. Mitri ◽  
Vagner Gonçalves Bernardo ◽  
Antonella M. Rossi ◽  
...  
Keyword(s):  
Bone Repair ◽  
Critical Size ◽  
Autogenous Bone ◽  
Control Group ◽  
Histomorphometric Analysis ◽  
Critical Size Defect ◽  
Treatment Groups ◽  
Alloplastic Materials ◽  
Significant Difference ◽  
Long Time

Biomaterials for treatment of bone defects have been studied for a long time. Alloplastic materials, mainly hydroxyapatite (HA), are under intense investigation due to its biocompatibility and osteoconductive properties. The HA can be modified by the incorporation of bivalent cations as Zn2+ known as a positive effectors for bone repair. The purpose of this study was to evaluate comparatively the effectiveness of 5% zinc-containing hydroxyapatite (ZnHA) in the treatment of critical size defect (CSD) in rat’s calvaria. CSD (8mm diameter) created in the skull of forty-five Wistar rats were filled with autogenous bone, HA and ZnHA. Skulls harvested after 30, 90 and 180 days were submitted to histological processing for paraffin embedding. Sections of 5 µm-thick stained with hematoxylin and eosin (HE) allowed histomorphometric analysis. The area of neoformed bone increased (p<0.001) from 30 to 180 days irrespective to treatment groups. ZnHA and the control group showed a large at 180 days but no significant difference compared to HA. Therefore, we concluded that both biomaterials are biocompatibles and osteoconductors, promoting new bone formation and apposition of bone on the surface throughout the periods and the addition of zinc improved the osteogenesis.

Histologic Evaluation of Osseous Regeneration Following Combination Therapy With Platelet-Rich Plasma and Bio-Oss in a Rat Calvarial Critical-Size Defect Model

2015 ◽  
Vol 41 (5) ◽  
pp. 543-549 ◽  
Author(s):  
Philip J. DeNicolo ◽  
M. Kelly Guyton ◽  
Michael F. Cuenin ◽  
Steven D. Hokett ◽  
Mohamed Sharawy ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Growth ◽  
Critical Size ◽  
Close Contact ◽  
Platelet Rich Plasma ◽  
Critical Size Defect ◽  
Histologic Evaluation ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain ◽  
Osseous Regeneration

Platelet-rich plasma (PRP) is an autogenous source of growth factors shown to facilitate human bone growth. Bio-Oss, an osteoconductive xenograft, is used clinically to regenerate periodontal defects, restore dental alveolar ridges, and facilitate sinus-lift procedures. The purpose of this study was to analyze whether a combination of PRP and Bio-Oss would enhance bone regeneration better than either material alone. PRP and/or Bio-Oss were administered in an 8-mm critical-size defect (CSD) rat calvarial model of bone defect between 2 polytetrafluoroethylene membranes to prevent soft tissue incursion. Eight weeks after the induction of the CSD, histologic sections were stained with hematoxylin and eosin stain and analyzed via light microscopy. Qualitative analyses revealed new bone regeneration in all 4 groups. The Bio-Oss and PRP plus Bio-Oss groups demonstrated greater areas of closure in the defects than the control or PRP-only groups because of the space-maintaining ability of Bio-Oss. The groups grafted with Bio-Oss showed close contact with new bone growth throughout the defects, suggesting a stronger graft. The use of PRP alone or in combination with Bio-Oss, however, did not appear to enhance osseous regeneration at 8 weeks. Areas grafted with Bio-Oss demonstrated greater space-maintaining capacity than controls, and PRP was an effective vehicle for placement of the Bio-Oss. However, at 8 weeks this study was unable to demonstrate a significant advantage of using PRP plus Bio-Oss over using Bio-Oss alone.

Influence of a titanium mesh on the management of segmental long bone defects

2015 ◽  
Vol 28 (06) ◽  
pp. 417-424 ◽  
Author(s):  
S. A. Papadimitriou ◽  
A. D. Galatos ◽  
N. N. Prassinos ◽  
D. Psalla ◽  
M. Dalstra ◽  
...  
Keyword(s):  
Critical Size ◽  
Experimental Studies ◽  
Long Bone ◽  
Histological Evaluation ◽  
Autogenous Bone ◽  
Bone Block ◽  
Titanium Mesh ◽  
Micro Computed Tomography ◽  
Critical Size Defect ◽  
Group B

SummaryObjectives: To evaluate the influence of titanium mesh on guided bone regeneration when used, either alone or in combination with autogenous bone block graft, in a canine ulnar model.Methods: Thirty-two, purpose bred, adult, castrated male Beagles were used, divided into four equal-size groups. A unilateral middiaphyseal ulnar critical-size defect was created in each dog. The ulnar segments were stabilized with a stainless-steel plate and screws. Each defect was managed by: no further treatment (Group A) or by placement of a bone block graft taken from the ipsilateral iliac crest (Group B), or titanium mesh wrapped around the ulna (Group C), or a bone block graft and titanium mesh (Group D). After six months, bone block biopsies were performed and the samples were scanned using micro-computed tomography. Qualitative histological evaluation was performed on two non-decalcified longitudinal sections from each block.Results: No significant differences in terms of mineralized bone volume were detected between the grafted sites (Groups B and D) or between the non-grafted ones (Groups A and C). The histological evaluation indicated good integration of the bone blocks irrespective of the use of titanium mesh.Clinical significance: The use of titanium mesh does not influence the amount of bone formation. The canine ulnar critical-size defect model seems to be a reliable model to use in experimental studies.

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