Intrauterine growth restriction leads to changes in sulfur amino acid metabolism, but not global DNA methylation, in Yucatan miniature piglets

2012 ◽  
Vol 23 (9) ◽  
pp. 1121-1127 ◽  
Author(s):  
Dylan S. MacKay ◽  
Julie D. Brophy ◽  
Laura E. McBreairty ◽  
Ross A. McGowan ◽  
Robert F. Bertolo
Keyword(s):  
Dna Methylation ◽  
Amino Acid ◽  
Intrauterine Growth Restriction ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Growth Restriction ◽  
Global Dna Methylation ◽  
Sulfur Amino Acid ◽  
Intrauterine Growth

Hepatic IGF1 DNA methylation is influenced by gender but not by intrauterine growth restriction in the young lamb

2015 ◽  
Vol 6 (6) ◽  
pp. 558-572 ◽  
Author(s):  
D. J. Carr ◽  
J. S. Milne ◽  
R. P. Aitken ◽  
C. L. Adam ◽  
J. M. Wallace
Keyword(s):  
Dna Methylation ◽  
Growth Hormone ◽  
Sexual Dimorphism ◽  
Mrna Expression ◽  
Intrauterine Growth Restriction ◽  
Messenger Rna ◽  
Methylation Status ◽  
Growth Restriction ◽  
Intrauterine Growth ◽  
Increased Risk

Intrauterine growth restriction (IUGR) and postnatal catch-up growth confer an increased risk of adult-onset disease. Overnourishment of adolescent ewes generates IUGR in ∼50% of lambs, which subsequently exhibit increased fractional growth rates. We investigated putative epigenetic changes underlying this early postnatal phenotype by quantifying gene-specific methylation at cytosine:guanine (CpG) dinucleotides. Hepatic DNA/RNA was extracted from IUGR [eight male (M)/nine female (F)] and normal birth weight (12 M/9 F) lambs. Polymerase chain reaction was performed using primers targeting CpG islands in 10 genes: insulin, growth hormone, insulin-like growth factor (IGF)1, IGF2, H19, insulin receptor, growth hormone receptor, IGF receptors 1 and 2, and the glucocorticoid receptor. Using pyrosequencing, methylation status was determined by quantifying cytosine:thymine ratios at 57 CpG sites. Messenger RNA (mRNA) expression of IGF system genes and plasma IGF1/insulin were determined. DNA methylation was independent of IUGR status but sexual dimorphism in IGF1 methylation was evident (M<F, P=0.008). IGF1 mRNA:18S and plasma IGF1 were M>F (both P<0.001). IGF1 mRNA expression correlated negatively with IGF1 methylation (r=−0.507, P=0.002) and positively with plasma IGF1 (r=0.884, P<0.001). Carcass and empty body weights were greater in males (P=0.002–0.014) and this gender difference in early body conformation was mirrored by sexual dimorphism in hepatic IGF1 DNA methylation, mRNA expression and plasma IGF1 concentrations.

Disorders of Sulfur Amino Acid Metabolism

2000 ◽  
pp. 224-231 ◽  
Author(s):  
Generoso Andria ◽  
Brian Fowler ◽  
Gianfranco Sebastio
Keyword(s):  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Sulfur Amino Acid

scholarly journals Methionine to cystine ratio in the total sulfur amino acid requirements and sulfur amino acid metabolism using labelled amino acid approach for broilers

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Letícia G. Pacheco ◽  
Nilva K. Sakomura ◽  
Rafael M. Suzuki ◽  
Juliano C. P. Dorigam ◽  
Gabriel S. Viana ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Sulfur Amino Acid ◽  
Total Sulfur ◽  
Amino Acid Requirements

scholarly journals Sulfur amino acid metabolism in children with severe childhood undernutrition: methionine kinetics

2006 ◽  
Vol 84 (6) ◽  
pp. 1400-1405 ◽  
Author(s):  
Farook Jahoor ◽  
Asha Badaloo ◽  
Marvin Reid ◽  
Terrence Forrester
Keyword(s):  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Sulfur Amino Acid ◽  
Childhood Undernutrition
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