Faculty Opinions recommendation of Experimental intrauterine growth restriction induces alterations in DNA methylation and gene expression in pancreatic islets of rats.

2010 ◽  
Author(s):  
Michael Symonds ◽  
Sylvain Sebert
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Pancreatic Islets ◽  
Intrauterine Growth Restriction ◽  
Growth Restriction ◽  
Intrauterine Growth

scholarly journals Experimental Intrauterine Growth Restriction Induces Alterations in DNA Methylation and Gene Expression in Pancreatic Islets of Rats

2010 ◽  
Vol 285 (20) ◽  
pp. 15111-15118 ◽  
Author(s):  
Reid F. Thompson ◽  
Melissa J. Fazzari ◽  
Hongshun Niu ◽  
Nir Barzilai ◽  
Rebecca A. Simmons ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Pancreatic Islets ◽  
Intrauterine Growth Restriction ◽  
Growth Restriction ◽  
Intrauterine Growth

Hepatic IGF1 DNA methylation is influenced by gender but not by intrauterine growth restriction in the young lamb

2015 ◽  
Vol 6 (6) ◽  
pp. 558-572 ◽  
Author(s):  
D. J. Carr ◽  
J. S. Milne ◽  
R. P. Aitken ◽  
C. L. Adam ◽  
J. M. Wallace
Keyword(s):  
Dna Methylation ◽  
Growth Hormone ◽  
Sexual Dimorphism ◽  
Mrna Expression ◽  
Intrauterine Growth Restriction ◽  
Messenger Rna ◽  
Methylation Status ◽  
Growth Restriction ◽  
Intrauterine Growth ◽  
Increased Risk

Intrauterine growth restriction (IUGR) and postnatal catch-up growth confer an increased risk of adult-onset disease. Overnourishment of adolescent ewes generates IUGR in ∼50% of lambs, which subsequently exhibit increased fractional growth rates. We investigated putative epigenetic changes underlying this early postnatal phenotype by quantifying gene-specific methylation at cytosine:guanine (CpG) dinucleotides. Hepatic DNA/RNA was extracted from IUGR [eight male (M)/nine female (F)] and normal birth weight (12 M/9 F) lambs. Polymerase chain reaction was performed using primers targeting CpG islands in 10 genes: insulin, growth hormone, insulin-like growth factor (IGF)1, IGF2, H19, insulin receptor, growth hormone receptor, IGF receptors 1 and 2, and the glucocorticoid receptor. Using pyrosequencing, methylation status was determined by quantifying cytosine:thymine ratios at 57 CpG sites. Messenger RNA (mRNA) expression of IGF system genes and plasma IGF1/insulin were determined. DNA methylation was independent of IUGR status but sexual dimorphism in IGF1 methylation was evident (M<F, P=0.008). IGF1 mRNA:18S and plasma IGF1 were M>F (both P<0.001). IGF1 mRNA expression correlated negatively with IGF1 methylation (r=−0.507, P=0.002) and positively with plasma IGF1 (r=0.884, P<0.001). Carcass and empty body weights were greater in males (P=0.002–0.014) and this gender difference in early body conformation was mirrored by sexual dimorphism in hepatic IGF1 DNA methylation, mRNA expression and plasma IGF1 concentrations.

Intrauterine growth restriction modifies the normal gene expression in kidney from rabbit fetuses

2012 ◽  
Vol 88 (11) ◽  
pp. 899-904 ◽  
Author(s):  
Horacio Figueroa ◽  
Mauricio Lozano ◽  
Cristian Suazo ◽  
Elisenda Eixarch ◽  
Sebastian E. Illanes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intrauterine Growth Restriction ◽  
Growth Restriction ◽  
Rabbit Fetuses ◽  
Intrauterine Growth ◽  
Normal Gene

Differences in gene expression dependent on sampling site in placental tissue of fetuses with intrauterine growth restriction

Placenta ◽  
2010 ◽  
Vol 31 (3) ◽  
pp. 178-185 ◽  
Author(s):  
A.A. Tzschoppe ◽  
E. Struwe ◽  
H.G. Dörr ◽  
T.W. Goecke ◽  
M.W. Beckmann ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intrauterine Growth Restriction ◽  
Sampling Site ◽  
Placental Tissue ◽  
Growth Restriction ◽  
Intrauterine Growth
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