Hind limb suspension and long-chain omega-3 PUFA increase mRNA endocannabinoid system levels in skeletal muscle

2012 ◽  
Vol 23 (8) ◽  
pp. 986-993 ◽  
Author(s):  
Heather L. Hutchins-Wiese ◽  
Yong Li ◽  
Kevin Hannon ◽  
Bruce A. Watkins
Keyword(s):  
Skeletal Muscle ◽  
Hind Limb ◽  
Endocannabinoid System ◽  
Omega 3 ◽  
Hind Limb Suspension ◽  
Long Chain

scholarly journals The Ubr2 gene is expressed in skeletal muscle atrophying as a result of hind limb suspension, but not Merg1a expression alone

2014 ◽  
Vol 24 (3) ◽  
Author(s):  
Gregory H. Hockerman ◽  
Nicole M. Dethrow ◽  
Sohaib Hameed ◽  
Maureen Doran ◽  
Christine Jaeger ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Hind Limb ◽  
Hind Limb Suspension

scholarly journals Erythrocyte Long Chain Omega 3 Fatty Acids Are Associated with Higher Lean Mass in Postmenopausal Women Newly Diagnosed with Breast Cancer (P05-024-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Martha Belury ◽  
Rachel Cole ◽  
Rebecca Andridge ◽  
Qing Xie ◽  
Ashleigh Keiter ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Skeletal Muscle ◽  
Fatty Acids ◽  
Body Composition ◽  
Lean Mass ◽  
Cardiometabolic Health ◽  
Omega 3 ◽  
Appendicular Lean Mass ◽  
Muscle Health ◽  
Long Chain

Abstract Objectives Cancer contributes to adverse changes in body composition that may increase risk of cardiometabolic diseases. Skeletal muscle is a main driver of cardiometabolic health. We hypothesize that higher intake of long chain omega-3 polyunsaturated fatty acids (LCn3PUFAs) is associated with muscle health. This study evaluates whether LCn3PUFA exposure assessed in diet and in blood is associated with markers of muscle health in women with breast cancer. Methods This is a cross-sectional analysis evaluating LCn3PUFA exposure and markers of muscle health in women (N = 150) prior to treatment for breast cancer. Exposure to LCn3PUFAs was assessed by a diet history questionnaire (DHQ), a question specifically about supplement usage and biomarker of LCn3PUFAs in red blood cells (RBC). Body composition were measured at the same visit using dual x-ray absorptiometry. Linear regression models were used to test for associations. Results 13% (N = 19) of women reported using fish oil supplements (e.g., Supplement Users) and had significantly higher levels of RBC LCn3PUFAs than Supplement Non-users. In Supplement Non-users, there was a positive association between reported dietary exposure by DHQ and RBC LCn3PUFA levels. The n3 index (e.g., sum of RBC EPA + DHA) and DHA (22:6n3) were positively associated with appendicular lean mass/BMI. There were no significant correlations between RBC LCn3PUFAs with grip strength and or other measurements of body composition. Conclusions As a biomarker of intake, RBC LCn3PUFAs are positively associated with appendicular lean mass, a measure of skeletal muscle mass. A future study should prospectively evaluate whether higher LCn3PUFA exposure (as measured in blood) is associated with maintaining better muscle health during and following cancer treatment. Funding Sources Funding was provided by the National Cattleman's Beef Association, NIH (JKG) CA186720, Ohio Agriculture Research and Development Center and the Carol S. Kennedy Professorship.

Mechanical properties of rat skeletal muscle after hind limb suspension

1987 ◽  
Vol 96 (3) ◽  
pp. 650-660 ◽  
Author(s):  
A.M. Winiarski ◽  
R.R. Roy ◽  
E.K. Alford ◽  
P.C. Chiang ◽  
V.R. Edgerton
Keyword(s):  
Mechanical Properties ◽  
Skeletal Muscle ◽  
Hind Limb ◽  
Rat Skeletal Muscle ◽  
Hind Limb Suspension

scholarly journals Dietary omega-3 and polyunsaturated fatty acids modify fatty acyl composition and insulin binding in skeletal-muscle sarcolemma.

1994 ◽  
Vol 299 (3) ◽  
pp. 831-837 ◽  
Author(s):  
S Liu ◽  
V E Baracos ◽  
H A Quinney ◽  
M T Clandinin
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acids ◽  
Saturated Fat ◽  
Insulin Binding ◽  
Fatty Acyl ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Acyl Composition ◽  
Fatty Acyl Composition ◽  
Long Chain

Feeding animals with diets high in saturated fat induces insulin resistance, and replacing saturated fat isocalorically with poly-unsaturated fat, especially long-chain omega-3 fatty acids, will prevent the development of insulin resistance in skeletal-muscle tissue. To investigate the mechanism, rats were fed on high-fat (20%, w/w) semipurified diets for 6 weeks. Diets containing ratios of polyunsaturated/saturated (P/S) fatty acid of 0.25 (low-P/S diet) and 1.0 (high-P/S diet) were used to study the effect of the level of saturated fat. To study the effects of omega-3 fatty acids, diets with a low-P/S ratio containing either 0 (low-omega-3 diet) or 3.3% (high-omega-3 diet) long-chain omega-3 fatty acids from fish oil were fed. Plasma membrane from skeletal muscle was purified. The content of fatty acids in sarcolemmal phospholipid was significantly related to the dietary composition. Insulin binding to intact sarcolemmal vesicles prepared from rats fed on diets high in omega-3 fatty acids increased 14-fold compared with animals fed on the low-omega-3 diet (P < 0.0001). Feeding rats on a diet with a high P/S ratio increased sarcolemmal insulin binding by 2.3-fold (P < 0.05). Increased insulin binding was due to increased receptor number at the low-affinity high-capacity binding site. Dietary effects on insulin binding were eliminated when studies were carried out on detergent-solubilized membranes, indicating the importance of the phospholipid fatty acyl composition for insulin binding. The results suggest that dietary omega-3 and polyunsaturated fatty acids increase insulin binding to sarcolemma by changing the fatty acyl composition of phospholipid surrounding the insulin receptor, and this might be the mechanism by which dietary fatty acids modify insulin action.

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