Lentiviral vectors carrying enhancer elements of Hb9 promoter drive selective transgene expression in mouse spinal cord motor neurons

2012 ◽  
Vol 205 (1) ◽  
pp. 139-147 ◽  
Author(s):  
Marco Peviani ◽  
Mami Kurosaki ◽  
Mineko Terao ◽  
Dario Lidonnici ◽  
Francesco Gensano ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Motor Neurons ◽  
Transgene Expression ◽  
Lentiviral Vectors ◽  
Mouse Spinal Cord

scholarly journals Single-cell transcriptomic analysis of the adult mouse spinal cord reveals molecular diversity of autonomic and skeletal motor neurons

2021 ◽  
Vol 24 (4) ◽  
pp. 572-583 ◽  
Author(s):  
Jacob A. Blum ◽  
Sandy Klemm ◽  
Jennifer L. Shadrach ◽  
Kevin A. Guttenplan ◽  
Lisa Nakayama ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Single Cell ◽  
Motor Neurons ◽  
Molecular Diversity ◽  
Adult Mouse ◽  
Transcriptomic Analysis ◽  
Mouse Spinal Cord

scholarly journals Chimeric rabies SADB19-VSVg-pseudotyped lentiviral vectors mediate long-range retrograde transduction from the mouse spinal cord

Gene Therapy ◽  
2015 ◽  
Vol 22 (5) ◽  
pp. 357-364 ◽  
Author(s):  
L Schoderboeck ◽  
S Riad ◽  
A M Bokor ◽  
H E Wicky ◽  
M Strauss ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Long Range ◽  
Lentiviral Vectors ◽  
Mouse Spinal Cord

scholarly journals The Temporal Mechanisms Guiding Interneuron Differentiation in the Spinal Cord

2021 ◽  
Vol 22 (15) ◽  
pp. 8025
Author(s):  
Dylan Deska-Gauthier ◽  
Ying Zhang
Keyword(s):  
Spinal Cord ◽  
Motor Neurons ◽  
The Other ◽  
Neuronal Diversity ◽  
Collective Knowledge ◽  
Mouse Spinal Cord ◽  
Developmental Mechanism ◽  
State Dependent ◽  
The Central Nervous System

Neurogenesis timing is an essential developmental mechanism for neuronal diversity and organization throughout the central nervous system. In the mouse spinal cord, growing evidence is beginning to reveal that neurogenesis timing acts in tandem with spatial molecular controls to diversify molecularly and functionally distinct post-mitotic interneuron subpopulations. Particularly, in some cases, this temporal ordering of interneuron differentiation has been shown to instruct specific sensorimotor circuit wirings. In zebrafish, in vivo preparations have revealed that sequential neurogenesis waves of interneurons and motor neurons form speed-dependent locomotor circuits throughout the spinal cord and brainstem. In the present review, we discuss temporal principals of interneuron diversity taken from both mouse and zebrafish systems highlighting how each can lend illuminating insights to the other. Moving forward, it is important to combine the collective knowledge from different systems to eventually understand how temporally regulated subpopulation function differentially across speed- and/or state-dependent sensorimotor movement tasks.

scholarly journals High-efficiency transduction of spinal cord motor neurons by intrauterine delivery of integration-deficient lentiviral vectors

2018 ◽  
Vol 273 ◽  
pp. 99-107 ◽  
Author(s):  
Sherif G. Ahmed ◽  
Simon N. Waddington ◽  
Maria Gabriela Boza-Morán ◽  
Rafael J. Yáñez-Muñoz
Keyword(s):  
Spinal Cord ◽  
Motor Neurons ◽  
High Efficiency ◽  
Lentiviral Vectors

Isolation and culture of motor neurons from the newborn mouse spinal cord

2004 ◽  
Vol 12 (3) ◽  
pp. 132-136 ◽  
Author(s):  
Kirstie N. Anderson ◽  
Allyson C. Potter ◽  
Loretta G. Piccenna ◽  
Alvin K.J. Quah ◽  
Kay E. Davies ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Motor Neurons ◽  
Newborn Mouse ◽  
Mouse Spinal Cord

scholarly journals High yield extraction of pure spinal motor neurons, astrocytes and microglia from single embryo and adult mouse spinal cord

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Marie-Josée Beaudet ◽  
Qiurui Yang ◽  
Sébastien Cadau ◽  
Mathieu Blais ◽  
Sabrina Bellenfant ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Motor Neurons ◽  
Adult Mouse ◽  
High Yield ◽  
Mouse Spinal Cord ◽  
Spinal Motor Neurons ◽  
Spinal Motor

scholarly journals Evidence for glutamine synthetase function in mouse spinal cord oligodendrocytes

2021 ◽  
Author(s):  
Lucile Ben Haim ◽  
Lucas Schirmer ◽  
Amel Zulji ◽  
Khalida Sabeur ◽  
Brice Tiret ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Glutamine Synthetase ◽  
Motor Neurons ◽  
Ventral Horn ◽  
Conditional Knockout ◽  
Oligodendrocyte Progenitor Cells ◽  
Mouse Spinal Cord ◽  
Reporter Mice ◽  
Key Enzyme ◽  
Ventral Spinal Cord

Glutamine synthetase (GS) is a key enzyme that metabolizes glutamate into glutamine. While GS is expressed by astrocytes of the central nervous system (CNS), expression in other glial lineages has been noted. Using a combination of reporter mice and cell type-specific markers, we show that GS is expressed in myelinating oligodendrocytes (OL) but not oligodendrocyte progenitor cells (OPC) of the mouse spinal cord abutting ventral horn motor neurons. To investigate the role of GS in mature OL, we used a conditional knockout (cKO) approach to selectively delete GS-encoding gene (Glul) in OL, which caused a significant decrease in glutamine levels on spinal cord extracts. We evaluated the effect on ventral spinal cord sensorimotor circuits and observed that GS cKO mice (CNP-cre+ : Glul fl/fl) showed no differences in motor neuron numbers, size or axon density; OL differentiation and myelination in the ventral spinal cord at 1- and 6-months of age was normal. Interestingly, GS cKO mice showed an early and specific decrease in peak force while motor function remained otherwise unaffected. These findings provide evidence OL-encoded GS functions in spinal cord sensorimotor circuit.

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