Quantitative analysis of immunofluorescent punctate staining of synaptically localized proteins using confocal microscopy and stereology

2006 ◽  
Vol 157 (2) ◽  
pp. 218-224 ◽  
Author(s):  
Maxim Mokin ◽  
Joyce Keifer
Keyword(s):  
Quantitative Analysis ◽  
Confocal Microscopy ◽  
Punctate Staining

scholarly journals Quantitative analysis of corneal microstructure in keratoconus utilising in vivo confocal microscopy

Eye ◽  
2006 ◽  
Vol 21 (5) ◽  
pp. 614-623 ◽  
Author(s):  
K H Weed ◽  
C J MacEwen ◽  
A Cox ◽  
C N J McGhee
Keyword(s):  
Quantitative Analysis ◽  
Confocal Microscopy ◽  
In Vivo Confocal Microscopy

Quantitative Analysis of Depth, Distribution, and Density of Cysts in Acanthamoeba Keratitis Using Confocal Microscopy

Cornea ◽  
2017 ◽  
Vol 36 (8) ◽  
pp. 927-932 ◽  
Author(s):  
Ping Huang ◽  
Tudor Tepelus ◽  
Laura A. Vickers ◽  
Elmira Baghdasaryan ◽  
Jianyan Huang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Confocal Microscopy ◽  
Depth Distribution ◽  
Acanthamoeba Keratitis

Activation-dependent and activation-independent localisation of calmodulin to the mitotic apparatus during the first cell cycle of the Lytechinus piçtus embryo.

Zygote ◽  
1995 ◽  
Vol 3 (3) ◽  
pp. 219-224 ◽  
Author(s):  
Martin Wilding ◽  
Katalin Török ◽  
Michael Whitaker
Keyword(s):  
Cell Cycle ◽  
Quantitative Analysis ◽  
Confocal Microscopy ◽  
Sea Urchin ◽  
Competitive Inhibitor ◽  
Mitotic Apparatus ◽  
Lytechinus Pictus ◽  
Calcium Dependent ◽  
Calmodulin Binding ◽  
Astral Microtubule

SummaryWe have used confocal microscopy and a fluorescent calmodulin probe to examine the mechanism of localisation of calmodulin during the first cell cycle of the sea urchin zygote. Using fluoresceincalmodulin, calmodulin can be observed within the nucleus and interphase astral microtubule arrays as cells approach mitosis. During mitosis, calmodulin redistributes to the mitotic apparatus and to condensed chromosomes. Quantitative analysis with reference to a control dye (fluorescein-dextran) shows that the distribution of calmodulin is specific. We used a competitive inhibitor of calcium-dependent calmodulin binding (Trp-peptide; Török & Trentham (1994) Biochemistry 33, 12807–20) to test whether the cell cycle localisation of calmodulin was due to its binding to targets on activation. The Trp-peptide eliminates localisation of calmodulin within the nucleus. However, microtubule localisation persists in the presence of the Trp-peptide. These data show that calmodulin can localise by calcium (and hence activation)-dependent as well as calcium-independent mechanisms. This suggests that distinct mechanisms of localisation may be involved in the regulation of the differential functions of calmodulin, at least during the cell cycle.

scholarly journals Preparation of the intact rodent organ of Corti for RNAscope and immunolabeling, confocal microscopy, and quantitative analysis

2021 ◽  
Vol 2 (2) ◽  
pp. 100544
Author(s):  
Daniel O.J. Reijntjes ◽  
J. Lukas Breitzler ◽  
Dora Persic ◽  
Sonja J. Pyott
Keyword(s):  
Quantitative Analysis ◽  
Confocal Microscopy ◽  
Organ Of Corti
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