An in vivo technique for pharmacological manipulation of Drosophila brain during optical recording

2006 ◽  
Vol 155 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Nicholas J.D. Wright
Keyword(s):  
Optical Recording ◽  
Pharmacological Manipulation ◽  
Drosophila Brain

scholarly journals In vivo classification of macaque foveal ganglion cells through optical recording of responses to chromatic and luminance flicker

2019 ◽  
Vol 19 (15) ◽  
pp. 19
Author(s):  
Tyler Godat ◽  
Juliette E. McGregor ◽  
Keith Parkins ◽  
William H. Merigan ◽  
David R. Williams
Keyword(s):  
Ganglion Cells ◽  
Optical Recording

scholarly journals Development of Optically-Controlled “Living Electrodes” with Long-Projecting Axon Tracts for a Synaptic Brain-Machine Interface

2018 ◽  
Author(s):  
Dayo O. Adewole ◽  
Laura A. Struzyna ◽  
James P. Harris ◽  
Ashley D. Nemes ◽  
Justin C. Burrell ◽  
...  
Keyword(s):  
Brain Activity ◽  
Optical Recording ◽  
Neural Interfaces ◽  
New Class ◽  
Brain Surface ◽  
Long Term Performance ◽  
Term Performance ◽  
The Brain

AbstractAchievements in intracortical neural interfaces are compromised by limitations in specificity and long-term performance. A biological intermediary between devices and the brain may offer improved specificity and longevity through natural synaptic integration with deep neural circuitry, while being accessible on the brain surface for optical read-out/control. Accordingly, we have developed the first “living electrodes” comprised of implantable axonal tracts protected within soft hydrogel cylinders for the biologically-mediated monitoring/modulation of brain activity. Here we demonstrate the controlled fabrication, rapid axonal outgrowth, reproducible cytoarchitecture, and simultaneous optical stimulation and recording of neuronal activity within these engineered constructs in vitro. We also present their transplantation, survival, integration, and optical recording in rat cortex in vivo as a proof-of-concept for this neural interface paradigm. The creation and functional validation of these preformed, axon-based “living electrodes” is a critical step towards developing a new class of biohybrid neural interfaces to probe and modulate native circuitry.

In vivo optical recording of neuronal activity of the superior colliculus in fetal rats

1998 ◽  
Vol 31 ◽  
pp. S179
Author(s):  
Yoshiyuki Sakata ◽  
Takashi Fujioka ◽  
Shoji Nakamura
Keyword(s):  
Superior Colliculus ◽  
Neuronal Activity ◽  
Optical Recording ◽  
Fetal Rats

scholarly journals Ca2+ clock malfunction in a canine model of pacing-induced heart failure

2010 ◽  
Vol 299 (6) ◽  
pp. H1805-H1811 ◽  
Author(s):  
Tetsuji Shinohara ◽  
Hyung-Wook Park ◽  
Seongwook Han ◽  
Mark J. Shen ◽  
Mitsunori Maruyama ◽  
...  
Keyword(s):  
Heart Failure ◽  
Heart Rate ◽  
Canine Model ◽  
Optical Recording ◽  
Crista Terminalis ◽  
Rate Acceleration ◽  
Intracellular Ca ◽  
Ectopic Beats ◽  
Rapid Ventricular Pacing

The mechanisms of sinoatrial node (SAN) dysfunction in heart failure (HF) remain unclear. We hypothesized that impaired rhythmic spontaneous sarcoplasmic reticulum Ca2+ release (Ca2+ clock) plays an important role in SAN dysfunction in HF. HF was induced in canine hearts by rapid ventricular pacing. The location of pacemaking sites was determined in vivo using computerized electrical mapping in acute open-chest preparations (normal, n = 3; and HF, n = 4). Isoproterenol (Iso, 0.2 μg·kg−1·min−1) infusion increased heart rate and shifted the pacemaking site to the superior SAN in all normal hearts. However, in failing hearts, Iso did not induce superior shift of the pacemaking site despite heart rate acceleration. Simultaneous optical recording of intracellular Ca2+ and membrane potential was performed in Langendorff-perfused isolated right atrium (RA) preparations from normal ( n = 7) and failing hearts ( n = 6). Iso increased sinus rate, enhanced late diastolic Ca2+ elevation (LDCAE), and shifted the pacemaking sites to the superior SAN in all normal but in none of the HF RAs. Caffeine (2 ml, 20 mmol/l) caused LDCAE and increased heart rate in four normal RAs but in none of the three HF RAs. Iso induced ectopic beats from lower crista terminalis in five of six HF RAs. These ectopic beats were suppressed by ZD-7288, a specific pacemaker current ( If) blocker. We conclude that HF results in the suppression of Ca2+ clock, resulting in the unresponsiveness of superior SAN to Iso and caffeine. HF also increases the ectopic pacemaking activity by activating the If at the latent pacemaking sites in lower crista terminalis.

scholarly journals Monitoring contractility in single cardiomyocytes and whole hearts with bio-integrated microlasers

2019 ◽  
Author(s):  
Marcel Schubert ◽  
Lewis Woolfson ◽  
Isla RM Barnard ◽  
Andrew Morton ◽  
Becky Casement ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Molecule ◽  
Muscle Activation ◽  
Cardiac Tissue ◽  
Frequency Comb ◽  
Cardiac Cells ◽  
Optical Recording ◽  
Cardiac Contraction

AbstractCardiac regeneration and stem cell therapies depend critically on the ability to locally resolve the contractile properties of heart tissue1,2. Current regeneration approaches explore the growth of cardiac tissue in vitro and the injection of stem cell-derived cardiomyocytes3–6 (CMs) but scientists struggle with low engraftment rates and marginal mechanical improvements, leaving the estimated 26 million patients suffering from heart failure worldwide without effective therapy7–9. One impediment to further progress is the limited ability to functionally monitor injected cells as currently available techniques and probes lack speed and sensitivity as well as single cell specificity. Here, we introduce microscopic whispering gallery mode (WGM) lasers into beating cardiomyocytes to realize all-optical recording of transient cardiac contraction profiles with cellular resolution. The brilliant emission and high spectral sensitivity of microlasers to local changes in refractive index enable long-term tracking of individual cardiac cells, monitoring of drug administration, and accurate measurements of organ scale contractility in live zebrafish. Our study reveals changes in sarcomeric protein density as underlying factor to cardiac contraction which is of fundamental importance for understanding the mechano-biology of cardiac muscle activation. The ability to non-invasively assess functional properties of transplanted cells and engineered cardiac tissue will stimulate the development of novel translational approaches and the in vivo monitoring of physiological parameters more broadly. Likewise, the use of implanted microlasers as cardiac sensors is poised to inspire the adaptation of the most advanced optical tools known to the microresonator community, like quantum-enhanced single-molecule biosensing or frequency comb spectroscopy10.

scholarly journals Study of the release of endogenous amines in Drosophila brain in vivo in response to stimuli linked to aversive olfactory conditioning

2020 ◽  
Author(s):  
Sergio Hidalgo ◽  
Nicolás Fuenzalida‐Uribe ◽  
Daniela Molina‐Mateo ◽  
Angélica P. Escobar ◽  
Carlos Oliva ◽  
...  
Keyword(s):  
Olfactory Conditioning ◽  
Drosophila Brain

scholarly journals All-optical recording and stimulation of retinal neurons in vivo in retinal degeneration mice

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0194947 ◽  
Author(s):  
Soon Keen Cheong ◽  
Jennifer M. Strazzeri ◽  
David R. Williams ◽  
William H. Merigan
Keyword(s):  
Retinal Degeneration ◽  
Optical Recording ◽  
Retinal Neurons ◽  
All Optical ◽  
Stimulation Of
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