In vitro digestion by saliva, simulated gastric and small intestinal juices and fermentation by human fecal microbiota of sulfated polysaccharides from Gracilaria rubra

2018 ◽  
Vol 40 ◽  
pp. 18-27 ◽  
Author(s):  
Tong Di ◽  
Guijie Chen ◽  
Yi Sun ◽  
Shiyi Ou ◽  
Xiaoxiong Zeng ◽  
...  
Keyword(s):  
In Vitro Digestion ◽  
Fecal Microbiota ◽  
Sulfated Polysaccharides ◽  
Small Intestinal

In vitro digestion of polysaccharides: InfoGest protocol and use of small intestinal extract from rat

2021 ◽  
Vol 140 ◽  
pp. 110054
Author(s):  
Pablo Gallego-Lobillo ◽  
Alvaro Ferreira-Lazarte ◽  
Oswaldo Hernández-Hernández ◽  
Mar Villamiel
Keyword(s):  
In Vitro Digestion ◽  
Small Intestinal

scholarly journals Assessing the in vitro digestion of Sesbania gum, a galactomannan from S. cannabina, and subsequent impact on the fecal microbiota

2021 ◽  
Vol 87 ◽  
pp. 104766
Author(s):  
Mengyi Zhou ◽  
Yuheng Tao ◽  
Ting Wang ◽  
Rong Wang ◽  
Qiang Yong
Keyword(s):  
In Vitro Digestion ◽  
Fecal Microbiota ◽  
Sesbania Gum

scholarly journals Physico-Chemical Characteristics and In Vitro Gastro-Small Intestinal Digestion of New Zealand Ryegrass Proteins

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 331
Author(s):  
Lovedeep Kaur ◽  
Harmandeepsingh Lamsar ◽  
Ignacio F. López ◽  
Manon Filippi ◽  
Dayna Ong Shu Min ◽  
...  
Keyword(s):  
New Zealand ◽  
Extraction Method ◽  
Acid Precipitation ◽  
In Vitro Digestion ◽  
Enzymatic Extraction ◽  
Physico Chemical ◽  
N Release ◽  
Food Applications ◽  
Small Intestinal

Being widely abundant, grass proteins could be a novel source of plant proteins for human foods. In this study, ryegrass proteins extracted using two different approaches-chemical and enzymatic extraction, were characterised for their physico-chemical and in vitro digestion properties. A New Zealand perennial ryegrass cultivar Trojan was chosen based on its higher protein and lower dry matter contents. Grass protein concentrate (GPC) with protein contents of approximately 55 and 44% were prepared using the chemical and enzymatic approach, respectively. The thermal denaturation temperature of the GPC extracted via acid precipitation and enzymatic treatment was found to be 68.0 ± 0.05 °C and 66.15 ± 0.03 °C, respectively, showing significant differences in protein’s thermal profile according to the method of extraction. The solubility of the GPC was highly variable, depending on the temperature, pH and salt concentration of the dispersion. The solubility of the GPC extracted via enzymatic extraction was significantly lower than the proteins extracted via the chemical method. Digestion of raw GPC was also studied via a gastro-small intestinal in vitro digestion model and was found to be significantly lower, in terms of free amino N release, for the GPC prepared through acid precipitation. These results suggest that the physico-chemical and digestion characteristics of grass proteins are affected by the extraction method employed to extract the proteins. This implies that selection of an appropriate extraction method is of utmost importance for achieving optimum protein functionality during its use for food applications.

scholarly journals In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4354
Author(s):  
Xin Zhou ◽  
Zhao Zhang ◽  
Fenghong Huang ◽  
Chen Yang ◽  
Qingde Huang
Keyword(s):  
Gut Microbiota ◽  
Reducing Sugars ◽  
In Vitro Digestion ◽  
Short Chain Fatty Acids ◽  
Fecal Microbiota ◽  
Gut Health ◽  
Human Gut ◽  
In Vitro Fermentation ◽  
Composition And Structure

The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota. FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact. Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota. FSP modulated the composition and structure of the gut microbiota by altering the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides. Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids. Our results indicate that FSP might be developed as a functional food that benefits gut health.

scholarly journals In Vitro Study for Lipolysis of Soybean Oil, Pomegranate Oil, and Their Blended and Interesterified Oils under a pH-Stat Model and a Simulated Model of Small Intestinal Digestion

Nutrients ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 678 ◽  
Author(s):  
Chenming Ji ◽  
Jung-Ah Shin ◽  
Soon Hong ◽  
Ki-Teak Lee
Keyword(s):  
Soybean Oil ◽  
In Vitro Study ◽  
In Vitro Digestion ◽  
Molar Ratio ◽  
Simulated Model ◽  
Interesterified Oil ◽  
Intestinal Digestion ◽  
Ph Stat ◽  
Small Intestinal

In this study, two in vitro digestion models were employed to compare the rate of lipolysis in soybean oil (SBO), pomegranate oil (PGO), a physical blend (PHY, 1:1 molar ratio of SBO:PGO, w/w), and their enzymatically interesterified oil (IO). In the pH-stat digestion model (emulsified oils with bile salts), PGO emulsion containing 74.7% conjugated form of linolenic acid (CLn) showed a significantly lower release rate of free fatty acid (FFA) than the other oil emulsions (p < 0.05). In FFA release rates and oil droplet sizes between PHY and IO emulsions, no significant differences were observed (p > 0.05). In a simulated model of small intestinal digestion, the lipolysis rates of SBO, PGO, PHY, and IO after digestion for 30 min in digestion fluids were 80.4%, 66.5%, 74.8%, and 77.0%, respectively. The rate of lipolysis in PGO was significantly lower than that in SBO (p < 0.05), and the lowest lipolysis rate was observed in the conjugated form of trilinolenoyl glycerol (CLn-CLn-CLn).

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