Intestinal anti-inflammatory effects of Passiflora edulis peel in the dextran sodium sulphate model of mouse colitis

2016 ◽  
Vol 26 ◽  
pp. 565-576 ◽  
Author(s):  
Cinthia Baú Betim Cazarin ◽  
Alba Rodriguez-Nogales ◽  
Francesca Algieri ◽  
M. Pilar Utrilla ◽  
M. Elena Rodríguez-Cabezas ◽  
...  
Keyword(s):  
Sodium Sulphate ◽  
Dextran Sodium Sulphate ◽  
Passiflora Edulis ◽  
Anti Inflammatory

scholarly journals The Effect of Ethanol Extract Turmeric Rhizome (Curcuma Longa) to Histologycal Structure of Rat Rectum Induced Dextran Sodium Sulphate (DSS)

2019 ◽  
Vol 20 (1) ◽  
pp. 13
Author(s):  
Lidia Maziyyatun Nikmah ◽  
Susantin Fajariyah ◽  
M. Mahriani
Keyword(s):  
Goblet Cell ◽  
Curcuma Longa ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Sodium Sulphate ◽  
Control Group ◽  
Dextran Sodium Sulphate ◽  
Anti Inflammatory ◽  
Group 1

Turmeric (Curcuma longa) is a plant that people use as a traditional medicine to treat some diseases such as: anti-inflammatory, antidiarrhea, antioxidants, hepatoprotector, and others. One of the main chemical compounds contained in turmeric and has a role as anti-inflammatory is curcumin. This study was conducted to determine the effect of ethanol extract of turmeric rhizome (Curcuma longa) on the histologycal structure of rat rectum induced by Dextran Sodium Sulphate (DSS). Wistar strain rats was divided into three groups: negative control group, positive control group (1% DSS), and DSS treatment group 1% + ethanol extract of turmeric rhizome dose 200mg/KgBB. DSS is given ad libitum, while ethanol extract of turmeric rhizome is given orally. We used paraffin method and Haematoxilyn Eosin staining to make the preparation organ. The data were analyzed using One Way ANOVA test, Duncan Multiple Range Test (DMRT) test, and correlation test. The result of this study is DSS administration has an effect on decreasing average of crypt, number of Goblet cell, and depletion of rectal mucous layer. While treatment of extract turmeric rhizome can increase height of crypt and number of Goblet cell in rectal mucosal layer of rat. Keywords: Exctract of turmeric rhizome, DSS, rectum, Rattus norvegicus

scholarly journals Beneficial Propionibacteria within a Probiotic Emmental Cheese: Impact on Dextran Sodium Sulphate-Induced Colitis in Mice

2020 ◽  
Vol 8 (3) ◽  
pp. 380 ◽  
Author(s):  
Houem Rabah ◽  
Fillipe Luiz Rosa do Carmo ◽  
Rodrigo Dias de Oliveira Carvalho ◽  
Barbara Fernandes Cordeiro ◽  
Sara Heloisa da Silva ◽  
...  
Keyword(s):  
Activity Index ◽  
Sodium Sulphate ◽  
Lactobacillus Delbrueckii ◽  
Interleukin 17 ◽  
Fermented Foods ◽  
Dextran Sodium Sulphate ◽  
Single Strain ◽  
Emmental Cheese ◽  
Anti Inflammatory ◽  
The Impact

Backgrounds and Aims. Inflammatory Bowel Diseases (IBD), including Ulcerative Colitis (UC), coincide with alterations in the gut microbiota. Consumption of immunomodulatory strains of probiotic bacteria may induce or prolong remission in UC patients. Fermented foods, including cheeses, constitute major vectors for bacteria consumption. New evidences revealed anti-inflammatory effects in selected strains of Propionibacterium freudenreichii. We thus hypothesized that consumption of a functional cheese, fermented by such a strain, may exert a positive effect on IBD. Methods. We investigated the impact of cheese fermented by P. freudenreichii on gut inflammation. We developed an experimental single-strain cheese solely fermented by a selected immunomodulatory strain of P. freudenreichii, CIRM-BIA 129. We moreover produced, in industrial conditions, an Emmental cheese using the same strain, in combination with Lactobacillus delbrueckii CNRZ327 and Streptococcus thermophilus LMD-9, as starters. Consumption of both cheeses was investigated with respect to prevention of Dextran Sodium Sulphate (DSS)-induced colitis in mice. Results. Consumption of the single-strain experimental cheese, or of the industrial Emmental, both fermented by P. freudenreichii CIRM-BIA 129, reduced severity of subsequent DSS-induced colitis, weight loss, disease activity index and histological score. Both treatments, in a preventive way, reduced small bowel Immunoglobulin A (IgA) secretion, restored occludin gene expression and prevented induction of Tumor Necrosis Factor α (TNFα), Interferon γ (IFNγ) and Interleukin-17 (IL-17). Conclusions. A combination of immunomodulatory strains of starter bacteria can be used to manufacture an anti-inflammatory cheese, as revealed in an animal model of colitis. This opens new perspectives for personalised nutrition in the context of IBD.

scholarly journals Titanium Dioxide Presents a Different Profile in Dextran Sodium Sulphate-Induced Experimental Colitis in Mice Lacking the IBD Risk Gene Ptpn2 in Myeloid Cells

2021 ◽  
Vol 22 (2) ◽  
pp. 772
Author(s):  
Javier Conde ◽  
Marlene Schwarzfischer ◽  
Egle Katkeviciute ◽  
Janine Häfliger ◽  
Anna Niechcial ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Titanium Dioxide ◽  
Genetic Risk ◽  
Intestinal Inflammation ◽  
Sodium Sulphate ◽  
Food Additive ◽  
Wild Type ◽  
Dextran Sodium Sulphate ◽  
Risk Gene ◽  
The Impact

Environmental and genetic factors have been demonstrated to contribute to the development of inflammatory bowel disease (IBD). Recent studies suggested that the food additive; titanium dioxide (TiO2) might play a causative role in the disease. Therefore, in the present study we aimed to explore the interaction between the food additive TiO2 and the well-characterized IBD risk gene protein tyrosine phosphatase non-receptor type 2 (Ptpn2) and their role in the development of intestinal inflammation. Dextran sodium sulphate (DSS)-induced acute colitis was performed in mice lacking the expression of Ptpn2 in myeloid cells (Ptpn2LysMCre) or their wild type littermates (Ptpn2fl/fl) and exposed to the microparticle TiO2. The impact of Ptpn2 on TiO2 signalling pathways and TiO2-induced IL-1β and IL-10 levels were studied using bone marrow-derived macrophages (BMDMs). Ptpn2LysMCre exposed to TiO2 exhibited more severe intestinal inflammation than their wild type counterparts. This effect was likely due to the impact of TiO2 on the differentiation of intestinal macrophages, suppressing the number of anti-inflammatory macrophages in Ptpn2 deficient mice. Moreover, we also found that TiO2 was able to induce the secretion of IL-1β via mitogen-activated proteins kinases (MAPKs) and to repress the expression of IL-10 in bone marrow-derived macrophages via MAPK-independent pathways. This is the first evidence of the cooperation between the genetic risk factor Ptpn2 and the environmental factor TiO2 in the regulation of intestinal inflammation. The results presented here suggest that the ingestion of certain industrial compounds should be taken into account, especially in individuals with increased genetic risk

scholarly journals Orally administered RDP58 reduces the severity of dextran sodium sulphate induced colitis

2002 ◽  
Vol 61 (Supplement 2) ◽  
pp. 19ii-24 ◽  
Author(s):  
R Boismenu ◽  
Y Chen ◽  
K Chou ◽  
A El-Sheikh ◽  
R Buelow
Keyword(s):  
Sodium Sulphate ◽  
Dextran Sodium Sulphate

scholarly journals Ablation of ceramide synthase 2 exacerbates dextran sodium sulphate-induced colitis in mice due to increased intestinal permeability

2017 ◽  
Vol 21 (12) ◽  
pp. 3565-3578 ◽  
Author(s):  
Ye-Ryung Kim ◽  
Giora Volpert ◽  
Kyong-Oh Shin ◽  
So-Yeon Kim ◽  
Sun-Hye Shin ◽  
...  
Keyword(s):  
Intestinal Permeability ◽  
Sodium Sulphate ◽  
Ceramide Synthase ◽  
Dextran Sodium Sulphate

Analysis of hypoxia-associated dendritic cells in colitic mice and effects of probiotics on IL-10 production in inflammatory dendritic-cells under hypoxia

2019 ◽  
Vol 10 (7) ◽  
pp. 801-810
Author(s):  
T. Ogita ◽  
J. Miyamoto ◽  
Y. Hirabayashi ◽  
M. Rossi ◽  
G. Mazzarella ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Hypoxia Inducible Factor ◽  
Flow Cytometric Analysis ◽  
Bifidobacterium Bifidum ◽  
Sodium Sulphate ◽  
Mesenteric Lymph Nodes ◽  
Cellular Mechanisms ◽  
Hypoxic Conditions ◽  
Anti Inflammatory

The aim of this study was to analyse hypoxia-associated dendritic cells (DCs) in colitic mice and the effects of probiotics on interleukin (IL)-10 production in inflammatory DCs under hypoxic conditions. Extensive hypoxia was observed in the colonic mucosa of dextran sodium sulphate-induced colitic mice. Flow cytometric analysis demonstrated that hypoxia-inducible factor-1α+ DCs in colonic lamina propria (CLP) lymphocytes and mesenteric lymph nodes (MLN) were more abundant in colitic mice than those in controls. Among three subsets of DCs, i.e. plasmacytoid DCs, conventional DCs (cDCs), and monocyte-derived DCs (mDCs), cDCs and mDCs were more abundant in CLP of colitic mice. Bone marrow-derived Flt-3L-induced DCs (Flt-DCs) but not bone marrow-derived GM-CSF-induced DCs (GM-DCs), incubated with 1% O2 exhibited an inflammatory phenotype, with higher CD86, IL-6, and tumour necrosis factor-α expression, and lower IL-10 levels than those in Flt-DCs incubated with 21% O2. The hypoxia-induced decrease in IL-10 expression in Flt-DCs was restored by Bifidobacterium bifidum JCM 1255T promoted IL-10 expression through the p38 pathway under normoxic conditions. The anti-inflammatory effects of B. bifidum JCM 1255T in Flt-DCs were mediated through different cellular mechanisms under hypoxic and normoxic conditions. B. bifidum JCM 1255T could be used therapeutically for its anti-inflammatory effects.

POTENTIAL OF ETHANOL EXTRACT OF MAHKOTA DEWA LEAVES (PHALERIA MACROCARPA (SCHECFF.) BOERL.) TO INHIBIT INFLAMMATION IN MOUSE DISTAL COLON INDUCED BY DEXTRAN SODIUM SULFATE (DSS) AND AZOXYMETHANE (AOM)

2020 ◽  
pp. 101-105
Author(s):  
MUHAMMAD ILHAM DHIYA RAKASIWI ◽  
KUSMARDI KUSMARDI ◽  
ARI ESTUNINGTYAS ◽  
ARYO TEDJO
Keyword(s):  
Leaf Extract ◽  
Histopathological Examination ◽  
Ethanol Extract ◽  
Distal Colon ◽  
Goblet Cells ◽  
Sodium Sulphate ◽  
Dextran Sodium Sulphate ◽  
Phaleria Macrocarpa ◽  
Hematoxylin Eosin

Objective: To demonstrates the ability of P. macrocarpa leaf extract to reduce inflammation of the distal colon in DSS/AOM-induced mice. Methods: In vivo experimental research using Balb/c mice induced by 0.2 ml azoxymethane (AOM) 0.1% once and 1% dextran sodium sulphate (DSS) for one week; additionally, ethanol extract of P. macrocarpa leaves, 25 mg and 50 mg, and 0.84 mg acetosal were given orally. The mice were sacrificed after 20 w. Histopathological examination (hematoxylin-eosin staining) was conducted by counting the average number of goblet cells per crypt, inflammatory focus and angiogenesis. Results: Ethanol extract of P. macrocarpa leaves was able to prevent the decrease in the number of goblet cells (p<0.05). However, the administration of ethanol P. macrocarpa leaf extract could not reduce focal inflammation and angiogenesis in inflammation of the distal colon. Conclusion: Ethanol extract of the Mahkota Dewa leaves is able to prevent inflammation of the distal colon by preventing the decrease in the number of goblet cells.

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