scholarly journals Inhibitory Activity Goblet Depletion and focal inflammatory Phaleria macrocarpha Leaves Ethanol Extract on Crypta Mouse after Dextran Sodium Sulphate Induction

2019 ◽  
Vol 12 (01) ◽  
pp. 37-48
Author(s):  
Rianti Maharani ◽  
Kusmardi ◽  
Berna Elya
Keyword(s):  
Inhibitory Activity ◽  
Ethanol Extract ◽  
Sodium Sulphate ◽  
Dextran Sodium Sulphate

POTENTIAL OF ETHANOL EXTRACT OF MAHKOTA DEWA LEAVES (PHALERIA MACROCARPA (SCHECFF.) BOERL.) TO INHIBIT INFLAMMATION IN MOUSE DISTAL COLON INDUCED BY DEXTRAN SODIUM SULFATE (DSS) AND AZOXYMETHANE (AOM)

2020 ◽  
pp. 101-105
Author(s):  
MUHAMMAD ILHAM DHIYA RAKASIWI ◽  
KUSMARDI KUSMARDI ◽  
ARI ESTUNINGTYAS ◽  
ARYO TEDJO
Keyword(s):  
Leaf Extract ◽  
Histopathological Examination ◽  
Ethanol Extract ◽  
Distal Colon ◽  
Goblet Cells ◽  
Sodium Sulphate ◽  
Dextran Sodium Sulphate ◽  
Phaleria Macrocarpa ◽  
Hematoxylin Eosin

Objective: To demonstrates the ability of P. macrocarpa leaf extract to reduce inflammation of the distal colon in DSS/AOM-induced mice. Methods: In vivo experimental research using Balb/c mice induced by 0.2 ml azoxymethane (AOM) 0.1% once and 1% dextran sodium sulphate (DSS) for one week; additionally, ethanol extract of P. macrocarpa leaves, 25 mg and 50 mg, and 0.84 mg acetosal were given orally. The mice were sacrificed after 20 w. Histopathological examination (hematoxylin-eosin staining) was conducted by counting the average number of goblet cells per crypt, inflammatory focus and angiogenesis. Results: Ethanol extract of P. macrocarpa leaves was able to prevent the decrease in the number of goblet cells (p<0.05). However, the administration of ethanol P. macrocarpa leaf extract could not reduce focal inflammation and angiogenesis in inflammation of the distal colon. Conclusion: Ethanol extract of the Mahkota Dewa leaves is able to prevent inflammation of the distal colon by preventing the decrease in the number of goblet cells.

Potential of Phaleria macrocarpa Leaves Ethanol Extract to Upregulate the Expression of Caspase-3 in Mouse Distal Colon after Dextran Sodium Sulphate Induction

2021 ◽  
Vol 13 (1) ◽  
pp. 23-29
Author(s):  
Kusmardi Kusmardi ◽  
Elvan Wiyarta ◽  
Ari Estuningtyas ◽  
Nurhuda Sahar ◽  
Yurnadi Hanafi Midoen ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Ethanol Extract ◽  
Distal Colon ◽  
Sodium Sulphate ◽  
Dextran Sodium Sulphate ◽  
Phaleria Macrocarpa

scholarly journals The Effect of Ethanol Extract Turmeric Rhizome (Curcuma Longa) to Histologycal Structure of Rat Rectum Induced Dextran Sodium Sulphate (DSS)

2019 ◽  
Vol 20 (1) ◽  
pp. 13
Author(s):  
Lidia Maziyyatun Nikmah ◽  
Susantin Fajariyah ◽  
M. Mahriani
Keyword(s):  
Goblet Cell ◽  
Curcuma Longa ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Sodium Sulphate ◽  
Control Group ◽  
Dextran Sodium Sulphate ◽  
Anti Inflammatory ◽  
Group 1

Turmeric (Curcuma longa) is a plant that people use as a traditional medicine to treat some diseases such as: anti-inflammatory, antidiarrhea, antioxidants, hepatoprotector, and others. One of the main chemical compounds contained in turmeric and has a role as anti-inflammatory is curcumin. This study was conducted to determine the effect of ethanol extract of turmeric rhizome (Curcuma longa) on the histologycal structure of rat rectum induced by Dextran Sodium Sulphate (DSS). Wistar strain rats was divided into three groups: negative control group, positive control group (1% DSS), and DSS treatment group 1% + ethanol extract of turmeric rhizome dose 200mg/KgBB. DSS is given ad libitum, while ethanol extract of turmeric rhizome is given orally. We used paraffin method and Haematoxilyn Eosin staining to make the preparation organ. The data were analyzed using One Way ANOVA test, Duncan Multiple Range Test (DMRT) test, and correlation test. The result of this study is DSS administration has an effect on decreasing average of crypt, number of Goblet cell, and depletion of rectal mucous layer. While treatment of extract turmeric rhizome can increase height of crypt and number of Goblet cell in rectal mucosal layer of rat. Keywords: Exctract of turmeric rhizome, DSS, rectum, Rattus norvegicus

scholarly journals Titanium Dioxide Presents a Different Profile in Dextran Sodium Sulphate-Induced Experimental Colitis in Mice Lacking the IBD Risk Gene Ptpn2 in Myeloid Cells

2021 ◽  
Vol 22 (2) ◽  
pp. 772
Author(s):  
Javier Conde ◽  
Marlene Schwarzfischer ◽  
Egle Katkeviciute ◽  
Janine Häfliger ◽  
Anna Niechcial ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Titanium Dioxide ◽  
Genetic Risk ◽  
Intestinal Inflammation ◽  
Sodium Sulphate ◽  
Food Additive ◽  
Wild Type ◽  
Dextran Sodium Sulphate ◽  
Risk Gene ◽  
The Impact

Environmental and genetic factors have been demonstrated to contribute to the development of inflammatory bowel disease (IBD). Recent studies suggested that the food additive; titanium dioxide (TiO2) might play a causative role in the disease. Therefore, in the present study we aimed to explore the interaction between the food additive TiO2 and the well-characterized IBD risk gene protein tyrosine phosphatase non-receptor type 2 (Ptpn2) and their role in the development of intestinal inflammation. Dextran sodium sulphate (DSS)-induced acute colitis was performed in mice lacking the expression of Ptpn2 in myeloid cells (Ptpn2LysMCre) or their wild type littermates (Ptpn2fl/fl) and exposed to the microparticle TiO2. The impact of Ptpn2 on TiO2 signalling pathways and TiO2-induced IL-1β and IL-10 levels were studied using bone marrow-derived macrophages (BMDMs). Ptpn2LysMCre exposed to TiO2 exhibited more severe intestinal inflammation than their wild type counterparts. This effect was likely due to the impact of TiO2 on the differentiation of intestinal macrophages, suppressing the number of anti-inflammatory macrophages in Ptpn2 deficient mice. Moreover, we also found that TiO2 was able to induce the secretion of IL-1β via mitogen-activated proteins kinases (MAPKs) and to repress the expression of IL-10 in bone marrow-derived macrophages via MAPK-independent pathways. This is the first evidence of the cooperation between the genetic risk factor Ptpn2 and the environmental factor TiO2 in the regulation of intestinal inflammation. The results presented here suggest that the ingestion of certain industrial compounds should be taken into account, especially in individuals with increased genetic risk

scholarly journals Orally administered RDP58 reduces the severity of dextran sodium sulphate induced colitis

2002 ◽  
Vol 61 (Supplement 2) ◽  
pp. 19ii-24 ◽  
Author(s):  
R Boismenu ◽  
Y Chen ◽  
K Chou ◽  
A El-Sheikh ◽  
R Buelow
Keyword(s):  
Sodium Sulphate ◽  
Dextran Sodium Sulphate

scholarly journals Ablation of ceramide synthase 2 exacerbates dextran sodium sulphate-induced colitis in mice due to increased intestinal permeability

2017 ◽  
Vol 21 (12) ◽  
pp. 3565-3578 ◽  
Author(s):  
Ye-Ryung Kim ◽  
Giora Volpert ◽  
Kyong-Oh Shin ◽  
So-Yeon Kim ◽  
Sun-Hye Shin ◽  
...  
Keyword(s):  
Intestinal Permeability ◽  
Sodium Sulphate ◽  
Ceramide Synthase ◽  
Dextran Sodium Sulphate

scholarly journals Metabolite and Elastase Activity Changes in Beach Rose (Rosa rugosa) Fruit and Seeds at Various Stages of Ripeness

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1283
Author(s):  
Seung-Hun Chae ◽  
Young-Sang Lee ◽  
Jin-Hee Kim ◽  
Tae-Ho Han ◽  
Kang-Mo Ku
Keyword(s):  
Fatty Acids ◽  
Ursolic Acid ◽  
Inhibitory Activity ◽  
Ethanol Extract ◽  
Dry Weight ◽  
Total Phenolic ◽  
Rosa Rugosa ◽  
Optimal Harvest ◽  
Rose Hip ◽  
The Rose

Rose hips are the fruits of the beach rose (Rosa rugosa). To determine the optimal harvest time and to obtain the maximum functional compounds, rose hips at various stages of ripeness (immature, early, mid, and late) were harvested, and the flesh tissue and seeds were separated. The rose hip flesh showed the highest total phenolic content at the mid-ripeness stage (8.45 ± 0.62 mg/g gallic acid equivalent concentration (dry weight)). The early-, mid-, and late-ripeness stages of rose hip flesh did not show significantly different 2,2-diphenyl-1-picrylhydrazyl antioxidant capacities. The elastase inhibitory activity of the 95% ethanol extract from the rose hip seeds was highest at the mid-ripeness stage; however, the elastase inhibitory activity of the rose hip tissue was not significantly different from that of the seeds. Pathway analysis using MetaboAnalyst showed that sucrose, fructose, and glucose gradually increased as the fruit ripened. Ursolic acid was detected in the seeds but not in the flesh. Of the fatty acids, linoleic acid concentrations were highest in rose hip seeds, followed by linolenic acid, oleic acid, and palmitic acid. Fatty acids and ursolic acid might be the active compounds responsible for elastase inhibitory activity and can be utilized as a functional cosmetic material.

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