scholarly journals α-Glucosidase and α-amylase inhibitory constituent of Carex baccans: Bio-assay guided isolation and quantification by validated RP-HPLC–DAD

2013 ◽  
Vol 5 (1) ◽  
pp. 211-218 ◽  
Author(s):  
Deepak Kumar ◽  
Nidhi Gupta ◽  
Rina Ghosh ◽  
Raghuvir H. Gaonkar ◽  
Bikas C. Pal
Keyword(s):  
Rp Hplc ◽  
Carex Baccans ◽  
Bio Assay

scholarly journals Bio-assay Guided Isolation of α-Glucosidase Inhibitory Constituents from Eclipta alba

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Deepak Kumar ◽  
Raghuvir H. Gaonkar ◽  
Rina Ghosh ◽  
Bikas C. Pal
Keyword(s):  
Diabetes Mellitus ◽  
Plant Extract ◽  
Quantitative Estimation ◽  
Eclipta Alba ◽  
Butanol Fraction ◽  
Echinocystic Acid ◽  
Rp Hplc ◽  
Bio Assay

Eclipta alba (L.) Hassk is used traditionally in diabetes mellitus in India and the plant extract is reported to possess anti-diabetic activity. A bioactivity-guided isolation approach based on α-glucosidase inhibition was used to identify the constituents contributing towards the inhibition of the enzyme and probably contributing towards its anti-diabetic activity. Four echinocystic acid glycosides were thus isolated, of which eclalbasaponin VI, isolated from the n-butanol fraction, was found to be the most potent (IC50 54.2 ± 1.3 μM). The compound is an uncompetitive type of inhibitor with Ki 26.1 μM. A quantitative estimation of the constituents was established using RP-HPLC.

RP-HPLC method development for simultaneous determination of the drugs Ramipril and Amlodipine

2012 ◽  
Vol 2 (2) ◽  
pp. 364-367 ◽  
Author(s):  
Saida Naik Dheeravath ◽  
◽  
Kasani Ramadevi ◽  
Zilla Saraswathi ◽  
Dheeravath Maniklal ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Method Development ◽  
Hplc Method ◽  
Rp Hplc ◽  
Hplc Method Development

RP-HPLC analysis of withanolides in the flowers, leaves, and roots ofWithania somnifera

2010 ◽  
Vol 22 (3) ◽  
pp. 473-480 ◽  
Author(s):  
N. W. Ali ◽  
S. Abouzid ◽  
A. Nasib ◽  
S. Khan ◽  
J. Qureshi ◽  
...  
Keyword(s):  
Hplc Analysis ◽  
Rp Hplc ◽  
Leaves And Roots

A comparative technique using as Joint studying to prove structure and determination the Quantitative estimation of organic compound (Irbesartan) as drug in multicomponent tablet form

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Imad Tarek Hanoon ◽  
Abed Mohammed Daheir AL-Joubory 2 ◽  
Marwa Mohamed Saied 3
Keyword(s):  
Mobile Phase ◽  
Chemical Structure ◽  
Quantitative Estimation ◽  
Potassium Phosphate ◽  
Hplc Method ◽  
Pharmaceutical Dosage Forms ◽  
Tablet Form ◽  
Rp Hplc ◽  
Percent Recovery

A simple , specific, accurate and precise RP-HPLC method was developed for determination of Irbesartan (IRB) in pharmaceutical dosage forms in tablets products and sachet using symmetry (L 1 ) column at 30°C . The signal was detected at 225 nm. A mobile phase dissolve 0.5 g of buffer potassium phosphate in 100 ml distilled water and adjust pH 2.7 , methanol and acetonitrile at ratio (40 :30 :30 ) . and flow rate 1.2ml/min -1 at pH=7.2 a mobile phase The percent recovery was detected 101 % and the linearity of concentration was 10-50 µg.ml -1 and supported this method by using (FT.I.R.) spectrum method for organic spectrophotometer to prove the chemical structure of this drug and some physical properties . we are obtained the result is identical of other literature . The proposed method was applied successfully for determination of the IRB in tablets products.

RP-HPLC ANALYSIS OF CARBONYL COMPOUNDS IN SOME INDOOR AIR SAMPLES IN A FACULTY FROM CLUJ-NAPOCA

2013 ◽  
Vol 12 (2) ◽  
pp. 219-225
Author(s):  
Carmen Roba ◽  
Cristina Rosu ◽  
Iulia Neamtiu ◽  
Eugen Gurzau
Keyword(s):  
Indoor Air ◽  
Carbonyl Compounds ◽  
Hplc Analysis ◽  
Air Samples ◽  
Rp Hplc
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