Embryo Transfer in Anovulatory Recipient Mares Treated with Estradiol Benzoate and Long-Acting Progesterone

2013 ◽  
Vol 33 (3) ◽  
pp. 205-209 ◽  
Author(s):  
Fernanda Kaercher ◽  
Luiz Ernandes Kozicki ◽  
Carlos Eduardo Camargo ◽  
Romildo Romualdo Weiss ◽  
Ivo Walter dos Santos ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Estradiol Benzoate ◽  
Long Acting

170 PREGNANCY RATES OF NONCYCLING RECIPIENT MARES TREATED WITH A NEW FORMULATION OF LONG-ACTING PROGESTERONE

2010 ◽  
Vol 22 (1) ◽  
pp. 243
Author(s):  
M. M. B. Castro Chaves ◽  
C. G. Schutzer ◽  
M. A. Alvarenga
Keyword(s):  
Embryo Transfer ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Control Group ◽  
Limiting Factor ◽  
Transfer Programs ◽  
Embryonic Loss ◽  
Exogenous Progesterone ◽  
Long Acting ◽  
New Formulation

The mare being a seasonal polyestrous animal is a limiting factor for maintenance of embryo transfer programs during the entire year.At the beginning and end of the breeding season, the percentage of recipients showing normal estrous cycles may be low compared to donors. Exogenous progesterone has been administered to acyclic mares to allow them to be used as recipients. Noncyclic recipient mares treated once a week with long-acting progesterone (P4) have been widely used in Brazil in equine embryo transfer (ET) programs. P4LA was developed for weekly administration of 1.5 g of P4 to acyclic recipients until approximately 120 days of pregnancy. The aim of the present experiment was to evaluate the pregnancy rates of noncyclic recipients treated with a new formulation of long-acting P4 developed in our laboratory that allows a longer interval between treatments (14 days). Noncyclic mares (n = 36) were used as recipients between August and September of 2008 (transition between winter and spring in Brazil). Noncyclic mares were treated with 5 mg of estradiol benzoate during 2 consecutive days followed by the application of 10 mL (i.m.) of P4LA containing 300 mg mL-1 of P4, for a total P4 dose of 3 g. Embryos were transferred between 6 to 12 days after P4LA injection. The same dose of P4LA was repeated on the day of ET and every 14 days until 110 days after pregnancy diagnosis. One hundred fifty-five cyclic recipients were used as a control group, with embryos transferred 4 to 8 days after ovulation. No differences were observed (P > 0.05) when comparing pregnancy rates from acyclic recipients treated with P4LA (82%, 56/68) or cyclic recipients (88%; 137/155) on Day 15. The rate of embryonic loss at 50 days was similar (P > 0.05) for noncycling (11%) and cycling recipient mares (10%). The results obtained in the present study demonstrated that the new formulation of P4LA containing 300 mg mL-1 of P4 and administered every 14 days was effective in maintaining pregnancy in noncyclic recipient mares, allowing a larger treatment interval and longer window of time to transfer embryos.

176 PREGNANCY AND PREGNANCY LOSS RATES OF ACYCLIC RECIPIENT MARES TREATED WITH TWO DIFFERENT LONG-ACTING PROGESTERONE CONCENTRATIONS

2010 ◽  
Vol 22 (1) ◽  
pp. 246 ◽  
Author(s):  
G. M. Greco ◽  
J. A. Dell'aqua Jr ◽  
M. A. Alvarenga
Keyword(s):  
Embryo Transfer ◽  
Pregnancy Loss ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Chi Square ◽  
Chi Square Test ◽  
Group 2 ◽  
Long Acting ◽  
Group 1 ◽  
Loss Rates

Long-acting progesterone compounds containing concentrations of 150 mg mL-1 (P4LA150) have been routinely used in Brazil, especially at the beginning and end of the breeding season when the percentage of recipient mares showing normal estrous cycles is low compared to donors (Greco GM et al. 2008 Proceedings 7th International Symposium on Equine Embryo Transfer, 59-60). However, recipients treated with P4LA150 must receive weekly administrations until pregnancies achieve 100 days. The present study aimed to evaluate pregnancy and pregnancy loss rates from acyclic mares treated at 7-day intervals with P4LA150 and 14-day intervals with a long-acting progesterone formulation containing 300 mg mL-1 (P4LA300). Acyclic Campolina breed mares (n = 80) ranging from 4 to 13 years old showing considerable endometrial edema 2 days after estradiol benzoate injection (5 mg, i.m.) were split in 2 groups. Group 1 mares (n = 40) received 10 mL of P4LA150 (1500 mg) i.m. every 7 days, whereas Group 2 mares (n = 40) were treated with 10 mL of P4LA300 (3000 mg) i.m. every 14 days. Steroid hormone formulations were produced in our research laboratory, at São Paulo State University, Botucatu, Brazil. Embryos 7 to 8 days of age were transferred into the uteri of recipients between 4 and 7 days after the first progesterone administration. Following a positive pregnancy diagnosis at 14 to 15 days, mares were examined at Days 50 and 100, when progesterone treatment was discontinued. These evaluations were conducted in order to determine pregnancy loss rates during both periods. Results were analyzed through the chi-square test and significance was set at P < 0.05. Pregnancy rates and pregnancy loss rates at Day 50 from mares treated with P4LA150 (Group 1) and P4LA300 (Group 2) did not differ. Pregnancy rates were 60.0% (24/40) and 75.0% (30/40) at Day 50 for mares treated with P4LA150 (Group 1) and P4LA300 (Group 2), respectively (P > 0.10). Pregnancy loss rates at Day 50 were 8.3% (2/24) and 10.0% (3/30) for mares treated with P4LA150 and P4LA300, respectively (P > 0.10). Pregnancy losses were not identified at Day 100. Based on the results from the present study, we concluded that acyclic recipient mares treated with P4LA150 or P4LA300 are able to obtain satisfactory pregnancy rates with low pregnancy losses. Moreover, the use of 3000 mg of long-acting progesterone every 14 days reduces overall progesterone administrations, minimizes stress in horse handling, and makes the choice for acyclic recipient mares more appealing in commercial embryo transfer programs. Authors would like to thank FAPESP for financial support.

Effect of the Timing of Frozen Embryo Transfer on Pregnancy Outcomes in High Ovarian Response Patients Undergoing Freeze-All Strategy

2020 ◽  
Author(s):  
Na Zuo ◽  
Yingzhuo Gao ◽  
Ningning Zhang ◽  
Da Li ◽  
Xiuxia Wang
Keyword(s):  
Propensity Score ◽  
Embryo Transfer ◽  
Subgroup Analysis ◽  
Pregnancy Outcomes ◽  
Ovarian Response ◽  
Frozen Embryo Transfer ◽  
Matched Cohort ◽  
Entire Cohort ◽  
Long Acting ◽  
Long Protocol

Abstract Background: Frozen embryo transfer (FET) can greatly improve the pregnancy outcomes for high ovarian response (HOR) population. However, it is not known whether the impaired endometrial receptivity derived from controlled ovarian hyperstimulation (COH) can be fully recovered in the first menstrual cycle after oocyte retrieval, and whether the timing of FET is a risk factor on pregnancy outcomes in HOR population undergoing freeze-all strategy.Methods: A retrospective cohort study to compare the pregnancy outcomes of the immediate and delayed FET groups in HOR population undergoing freeze-all strategy. Propensity score matching was used to make the potential risk factors of the immediate and delayed FET groups comparable. Multivariable regression analysis was used to study the effect of the timing of FET on pregnancy outcomes in the entire cohort and propensity score-matched cohort, even in different COH protocol cohorts as subgroup analysis.Results: We showed that the immediate FET group were no worse than delayed FET group in the entire cohort [clinical pregnancy rate (CPR), adjusted odd ratio (OR), 0.942, 95% confidence interval (CI), 0.784-1.133; spontaneous abortion rate (SAR), adjusted OR, 1.118, 95% Cl (0.771-1.623); live birth rate (LBR), adjusted OR, 1.060, 95% Cl (0.886-1.267)]. The same results were obtained by χ2 test in the propensity score-matched cohort (CPR, 60.5% versus 63.5%; SAR, 11.6% versus 12.3%; LBR, 48% versus 49.3%) (P > 0.05). Subgroup analysis indicated that pregnancy outcomes of immediate FET were non-inferior to delayed FET in short-acting gonadotropin-releasing hormone agonist (GnRH-a) long protocol (P > 0.05). The SAR of the immediate FET group were lower than that of the delayed FET group in GnRH antagonist protocol (adjusted OR, 0.646, 95% CI, 0.432-0.966) and long-acting GnRH-a long protocol (adjusted OR, 0.375, 95% CI, 0.142-0.990) (P < 0.05), no differences were observed in CPR and LBR (P > 0.05).Conclusions: These findings indicate that immediate FET might not affect pregnancy outcomes in HOR patients undergoing freeze-all strategy. Delaying FET could increase the SAR in GnRH-ant and long-acting GnRH-a long protocols.

178 USE OF A NEW INTRAVAGINAL DEVICE TO RELEASE PROGESTERONE IN FIXED-TIME EMBRYO TRANSFER EMBRYO RECIPIENTS

2010 ◽  
Vol 22 (1) ◽  
pp. 247
Author(s):  
J. R. V. Pimentel ◽  
P. H. P. Miguez ◽  
A. Kehrle ◽  
E. H. Madureira ◽  
F. A. Braga ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Body Condition Score ◽  
Block Design ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
P Value ◽  
São Paulo ◽  
Condition Score ◽  
Intravaginal Device

Progestagen-releasing devices are commonly used for fixed-time artificial insemination and fixed-time embryo transfer (FTET). The aim of this study was to evaluate the efficacy of a new progesterone-releasing intravaginal device made of poly(3-hydroxy)butyrate-valerate copolymer (PHB-V) and poly-ϵ-caprolactone (PCL; Progestar®, Innovare, São Paulo, Brazil) to synchronize crossbredcows and heifers for FTET by determining ovulation, conception, and pregnancy rates. A total of 205 recipients ranging in age from 2 to 8 years (heifers, n = 77; non-suckled cows, n = 69; suckled cows, n = 59) were used on 3 different farms during February, March, and April 2008. The experimental design was a generalized block design (cyclicity, body condition score, postpartum interval, and animal category, i.e. heifers, suckled, or non-suckled cows), with 2 different synchronization treatments for FTET (Progestar® v. Crestar®). Cows were assigned to either Crestar® (norgestomet ear implants; Intervet Schering-Plough, Kenilworth, NJ, USA; n = 103) or Progestar® (PHB-V; n = 102) treatments. All groups were treated with 2 mg of estradiol benzoate i.m. (Innovare) and received the intravaginal or auricular device according to their group (Day 0). Together with P4 device withdrawal (Day 8), all animals received 400 IU of eCG i.m. (Novormon®, Schering-Plough) and 500 μg i.m. of PGF2α, (Sincrocio®, Ouro Fino, Brasil). On Day 9, all cows were treated with 1 mg of estradiol benzoate (Innovare) and the innovulation was performed on Day 17. Embryos were maintained at 37°C during the innovulation period. The percentage of usable recipients (AR) based on the presence of a functional CL on the day of embryo transfer, conception rate (numbers of pregnant recipients of the total number transferred; CR), and pregnancy rate (numbers of pregnant recipients of the total number synchronized; PR) were determined by ultrasonography 23 days after embryo transfer. Data were submitted to ANOVA (PROC GLM, SAS Institute Inc., Cary, NC, USA) and significant differences were considered to have a P-value of <0.05. None of the interactions were significant and there was no effect of treatment on AR (72.8% v. 83.3%), CR (47.9% v. 42.7%), and PR (34.7% v. 35.0%) for Progestar® v. Crestar® groups, respectively. This experiment showed that Progestar® was as efficacious as Crestar® to synchronize crossbred cows and heifers for FTET. FAPESP-Fundação de Amparo à Pesquisa do Estado de São Paulo and CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico.

scholarly journals 166 EFFECT OF TREATMENT WITH hCG OR GnRH AT THE TIME OF EMBRYO TRANSFER ON PREGNANCY RATES IN COWS SYNCHRONIZED WITH PROGESTERONE VAGINAL DEVICES, ESTRADIOL BENZOATE, AND eCG

2005 ◽  
Vol 17 (2) ◽  
pp. 234 ◽  
Author(s):  
R. Tribulo ◽  
E. Balla ◽  
L. Cutaia ◽  
G.A. Bo ◽  
P.S. Baruselli ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Condition Score ◽  
Pregnancy Status ◽  
To Receive

Although several studies have investigated the relationship between circulating progesterone and pregnancy rates in cattle, the beneficial effect of treatments that increase progesterone concentrations, by insertion of a progesterone (P4) releasing device or induction of an accessory CL with hCG, GnRH, or LH treatment, has resulted in inconsistent effects on pregnancy rates in embryo recipients. An experiment was designed to evaluate the effect of hCG or GnRH treatment, given at the time of embryo transfer without estrus detection, on pregnancy rates in recipients treated with intrauterine P4-releasing devices, estradiol benzoate (EB), and eCG. The experiment was performed in two replicates; non-lactating Bos taurus × Bos indicus crossbred beef cows with a body condition score between 2.5 to 3.5 (1-to-5 scale) were used (replicate 1, n = 180; replicate 2, n = 140). All cows received 1 g of P4 via a P4-releasing device (DIB, Syntex, Argentina) and 2 mg EB i.m. (Syntex) on Day 0, and 400 IU of eCG i.m. (Novormon 5000, Syntex) plus 150 μg d(+)cloprostenol i.m. (Ciclase, Syntex) on Day 5. DIBs were removed on Day 8 and all cows received 1 mg EB i.m. on Day 9. Recipients were not observed for signs of estrus, and those >1 CL, or a single CL with an area >256 mm2, received 195 Grade 1 and 46 Grade 2 frozen/thawed “direct transfer” embryos on Day 17. At the time of embryo transfer, recipients were randomly allocated to 1 of 3 treatment groups to receive 1500 IU hCG (Ovusyn, Syntex), 50 μg Lecirelina (GnRH, Gonasyn, Syntex), or no treatment (control) at that time. Ovarian ultrasonography was performed on Day 0 to determine ovarian status (only cows with a CL or a follicle >10 mm and uterine tone were used), on Day 17 to measure CL area, and 40 days after embryo transfer to determine pregnancy status. Data were analyzed by logistic regression and the effects of replication, technician, treatment, and embryo quality were considered in the model. From the 320 recipients treated with a DIB plus EB and eCG, 241 (75.3%) were selected to receive an embryo. Nine (3.7%) and 1 (0.4%) of the selected recipients had 2 and 3 CL, respectively. Pregnancy rates did not differ between replicates (replicate 1: 80/140, 57.1%; and replicate 2: 57/101, 56.4%; P = 0.84), technicians (technician 1: 65/118, 55.1%; and technician 2: 72/123, 58.5%; P = 0.64), or treatments (hCG: 43/80, 53.8%; GnRH: 45/83, 54.2%; and control: 49/78, 62.8% P = 0.99). However, pregnancy rates were higher (P = 0.001) in recipients receiving Grade 1 embryos (121/195, 62.1%) than in those receiving Grade 2 embryos (16/46, 34.8%). GnRH or hCG treatment at the time of embryo transfer did not increase pregnancy rates in recipients synchronized with P4 releasing devices, EB, and eCG. Research was supported by Syntex S.A., Estancia El Mangrullo S.A., and Agencia Cordoba Ciencia S.E.

scholarly journals Gestational losses after innovating with two embryos in a fixed-time embryo transfer program

2021 ◽  
Vol 14 (10) ◽  
Author(s):  
Carla Neves Barbosa ◽  
Felipe De Col ◽  
Juan Carlos Domínguez Fernández de Tejerina ◽  
Rodolfo Cassimiro de Araujo Berber
Keyword(s):  
Embryo Transfer ◽  
Birth Rate ◽  
Considerable Increase ◽  
Hormonal Treatment ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Corpora Lutea ◽  
Transfer Program ◽  
Embryo Transfer Program ◽  
Group 1

The aim of this study was to quantify the pregnancy rate after implantation of two embryos after FTET protocols, as well as to monitor pregnancy losses until parturition, evaluating, mainly, if this strategy results in more number of animals born. Therefore, 423 multiparous recipients were selected, standardized in terms of body score, who had high-quality corpora lutea. Animals were randomly divided into two groups according to one or two embryos transferred (1 embryo = Control, n = 237; 2 embryos = Group 1, n = 186). All recipients received the same hormonal treatment, which consisted of administering, on Day 0, 2 mL of estradiol benzoate (Gonadiol, ZOETIS) + 1.9 g multidose 1st use progesterone implant (CIDR, ZOETIS); on Day 8 the implants were removed + injected 0.4 mL of estradiol cypionate (E.C.P, ZOETIS) + 1.5 mL of eCG (Novormon, ZOETIS) + 1 mL of dinoprost tromethamine (Lutalyse, ZOETIS). The animals were evaluated by ultrasonography at 30 and 60 days after embryo transfer, to diagnose the success rate and embryo losses during this period. Furthermore, information was collected on births, length of gestation, number of twin births, number of childbirth assistance and the weight of the calves. The results showed that Group 1 had better success than the Control, with higher conception rates at 30 days (68.3% vs. 53.2%, P<0.001) and at 60 days (62.9% vs. 52.3%; P<0 .05). The number of animals born was also higher for Group 1 (53.3% vs. 43.3%, P<0.01). The percentage of twins born in Group 1 was 17.9%, and the animals had lower weight compared to the Control (34.29 + 7.36 vs 37.63 + 5.73, P<0.05). The length of pregnancy and the number of assistances were similar between groups. In conclusion, the strategy adopted in this experiment suggests a considerable increase in the calf birth rate, but losses during pregnancy and their mechanisms need to be elucidated.

scholarly journals Evaluation of follicular growth and progesterone production in Bos taurus cows strategically treated with insulin

2019 ◽  
Vol 40 (5Supl1) ◽  
pp. 2287
Author(s):  
Andressa Stein Maffi ◽  
Mityelle da Costa Chaves Rodrigues ◽  
Lucas Balinhas Farias ◽  
Joabel Tonellotto dos Santos ◽  
Gabriela Bueno Luz ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Artificial Insemination ◽  
Bos Taurus ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Control Group ◽  
Follicular Growth ◽  
Insulin Group ◽  
Vaginal Device

The goal of this study was to evaluate the effects of strategic insulin administration on follicular growth, progesterone production, and pregnancy rate in beef cattle. Two experiments were conducted, in experiment 1, 215 cows crossbreed cows (Bos indicus × Bos taurus) (Control Group, n = 109; Insulin Group, n = 106) were submitted to fixed-time artificial insemination (TAI) with application of 2 mg of estradiol benzoate and a vaginal device containing progesterone on day 0. On day 9, the device was removed and 0.5 mg of estradiol cypionate was administered with 12.5 mg of dinoprost and 0.25 UI kg-1 of insulin for the treated animals. On day 11, artificial insemination (AI) was performed on all animals. On days 9 and 11 according to the protocol, was evaluated follicular growth and estrus manifestation, and 30 days after AI the pregnancy rate. In experiment 2, 85 cows were utilized, including Aberdeen Angus, Hereford, and crossbreeds (Control Group, n = 49 and Insulin Group, n = 36), and were submitted to the protocol for fixed-time embryo transfer (FTET), and progesterone concentrations were evaluated. On day 0, the cows received 2 mg of estradiol benzoate and a vaginal device containing progesterone. On day 5 of the protocol, 0.150 mg of cloprostenol was administered. On day 8, the vaginal device was removed and 20 mg of purified porcine pituitary extract and 0.25 UI kg-1 of insulin was given to the animals in the treatment group, and on day 9, 1 mg of estradiol benzoate was administered. On day 17, an embryo transfer was performed and blood collection for evaluation of progesterone levels in animals submitted to FTET were collected. In experiment 1, the follicular growth rate and the pregnancy rate were similar between groups (P > 0.05) and in experiment 2, the production of P4 was not different between the animals that received the insulin application and the control group. The utilization of a single dose of insulin for beef cows did not affect the ovulatory follicular diameter, progesterone concentrations, or pregnancy rate.

scholarly journals Short communication: Establishment and maintenance of donkey-in-mule pregnancy after embryo transfer in a non-cycling mule treated with oestradiol benzoate and long-acting progesterone

2018 ◽  
Vol 15 (4) ◽  
pp. e04SC01 ◽  
Author(s):  
Marina Bottrel ◽  
Thomaz Fortes ◽  
Isabel Ortiz ◽  
Manuel Hidalgo ◽  
Jesús Dorado
Keyword(s):  
Embryo Transfer ◽  
Short Communication ◽  
Progesterone Treatment ◽  
Oestradiol Benzoate ◽  
Long Acting ◽  
First Time

Female mules are considered as infertile; however, they could be used as recipients in interspecific embryo transfer. This study reports for the first time how it is possible to obtain the birth of a live Andalusian donkey foal after transfer a donkey embryo to a non-cycling mule. Two non-cycling mules were used as recipients, oestradiol benzoate was administered when donors showed oestrus and long-acting progesterone after ovulation. The mules also received long-acting progesterone every 7 days until 120 days of gestation. One embryo was collected from the two donor jennies and transferred to one of the mules after 5 days of progesterone treatment. Pregnancy was established and maintained after embryo transfer. The pregnant mule carried to term and delivered a live donkey foal after 375 days of pregnancy. In conclusion, non-cycling mules treated with oestradiol benzoate and long-acting progesterone can be successfully used as recipients of donkey embryos, which open new ways for the conservation of endangered donkey species.

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