scholarly journals 166 EFFECT OF TREATMENT WITH hCG OR GnRH AT THE TIME OF EMBRYO TRANSFER ON PREGNANCY RATES IN COWS SYNCHRONIZED WITH PROGESTERONE VAGINAL DEVICES, ESTRADIOL BENZOATE, AND eCG

2005 ◽  
Vol 17 (2) ◽  
pp. 234 ◽  
Author(s):  
R. Tribulo ◽  
E. Balla ◽  
L. Cutaia ◽  
G.A. Bo ◽  
P.S. Baruselli ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Condition Score ◽  
Pregnancy Status ◽  
To Receive

Although several studies have investigated the relationship between circulating progesterone and pregnancy rates in cattle, the beneficial effect of treatments that increase progesterone concentrations, by insertion of a progesterone (P4) releasing device or induction of an accessory CL with hCG, GnRH, or LH treatment, has resulted in inconsistent effects on pregnancy rates in embryo recipients. An experiment was designed to evaluate the effect of hCG or GnRH treatment, given at the time of embryo transfer without estrus detection, on pregnancy rates in recipients treated with intrauterine P4-releasing devices, estradiol benzoate (EB), and eCG. The experiment was performed in two replicates; non-lactating Bos taurus × Bos indicus crossbred beef cows with a body condition score between 2.5 to 3.5 (1-to-5 scale) were used (replicate 1, n = 180; replicate 2, n = 140). All cows received 1 g of P4 via a P4-releasing device (DIB, Syntex, Argentina) and 2 mg EB i.m. (Syntex) on Day 0, and 400 IU of eCG i.m. (Novormon 5000, Syntex) plus 150 μg d(+)cloprostenol i.m. (Ciclase, Syntex) on Day 5. DIBs were removed on Day 8 and all cows received 1 mg EB i.m. on Day 9. Recipients were not observed for signs of estrus, and those >1 CL, or a single CL with an area >256 mm2, received 195 Grade 1 and 46 Grade 2 frozen/thawed “direct transfer” embryos on Day 17. At the time of embryo transfer, recipients were randomly allocated to 1 of 3 treatment groups to receive 1500 IU hCG (Ovusyn, Syntex), 50 μg Lecirelina (GnRH, Gonasyn, Syntex), or no treatment (control) at that time. Ovarian ultrasonography was performed on Day 0 to determine ovarian status (only cows with a CL or a follicle >10 mm and uterine tone were used), on Day 17 to measure CL area, and 40 days after embryo transfer to determine pregnancy status. Data were analyzed by logistic regression and the effects of replication, technician, treatment, and embryo quality were considered in the model. From the 320 recipients treated with a DIB plus EB and eCG, 241 (75.3%) were selected to receive an embryo. Nine (3.7%) and 1 (0.4%) of the selected recipients had 2 and 3 CL, respectively. Pregnancy rates did not differ between replicates (replicate 1: 80/140, 57.1%; and replicate 2: 57/101, 56.4%; P = 0.84), technicians (technician 1: 65/118, 55.1%; and technician 2: 72/123, 58.5%; P = 0.64), or treatments (hCG: 43/80, 53.8%; GnRH: 45/83, 54.2%; and control: 49/78, 62.8% P = 0.99). However, pregnancy rates were higher (P = 0.001) in recipients receiving Grade 1 embryos (121/195, 62.1%) than in those receiving Grade 2 embryos (16/46, 34.8%). GnRH or hCG treatment at the time of embryo transfer did not increase pregnancy rates in recipients synchronized with P4 releasing devices, EB, and eCG. Research was supported by Syntex S.A., Estancia El Mangrullo S.A., and Agencia Cordoba Ciencia S.E.

scholarly journals 158 PREGNANCY RATES IN RECIPIENT COWS TREATED WITH PROGESTERONE VAGINAL DEVICES AND INDUCED TO OVULATE WITH ESTRADIOL BENZOATE GIVEN AT THE TIME OF DEVICE REMOVAL OR 24 h LATER

2005 ◽  
Vol 17 (2) ◽  
pp. 229 ◽  
Author(s):  
G.A. Bo ◽  
E. Balla ◽  
L. Cutaia ◽  
L. Peres ◽  
P.S. Baruselli ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Device Removal ◽  
The Mean ◽  
To Receive

Although treatments with progesterone (P4) releasing devices, estradiol benzoate (EB) and eCG have been shown to result in acceptable pregnancy rates after embryo transfer, the treatment requires that the cows be run through the chute at least four times for treatments. An experiment was designed to compare pregnancy rates in cows treated with P4 releasing devices plus EB and eCG, and induced to ovulate with EB given at device removal or 24 h later. Non-lactating Bos taurus × Bos indicus crossbred beef cows (n = 165), with a body condition score between 2.5 to 3.5 (1 to 5 scale), were treated with a P4-device (DIB, Syntex, Argentina) and 2 mg EB i.m. (Syntex), on Day 0 and 400 IU of eCG i.m. (Novormon, Syntex) plus 150 μg D(+)cloprostenol i.m. (Ciclase, Syntex) on Day 5. On Day 8, DIB devices were removed and cows were randomly divided into two groups to receive either 1 mg EB i.m. at the time of DIB removal (EB0) or 24 h later (Day 9; EB24). Recipients were observed for signs of estrus for 48 h after DIB removal. On Day 16, all recipients observed in estrus and with >1 CL or a single CL with an area >256 mm2 were selected to receive fresh embryos on Day 16 (EB0) or Day 17 (EB24). Furthermore, 20 recipients not observed in estrus but with a CL >256 mm2 were randomly selected and transferred. The embryos used were 28 Grade 1, 40 Grade 2, and 24 Grade 3. Ovarian ultrasonography was performed on Day 0, to determine ovarian status (only cows with a CL or a follicle >10 mm and uterine tone were used), on Day 16 to measure CL area, and 60 days after embryo transfer to determine pregnancy status. Quantitative data were analyzed by Student's t-test and qualitative data were analyzed by logistic regression. There were no differences between groups in the mean (± SEM) CL area on Day 16 (EB0: 300.3 ± 12.0 mm2 and EB24: 324.9 ± 11.7 mm2; P = 0.14), the proportion of recipients in estrus (EB0: 38/82, 46.3%, and EB24: 34/83, 41.0%; P = 0.49), the number of recipients with >1 CL or a CL >256 mm2 (EB0: 68/82, 82.9%, and EB24: 72/83, 86.7%; P = 0.49) and the number of recipients pregnant/transferred (EB0: 31/49, 63.4%, and 20/43, 46.5%; P = 0.23). Furthermore, there were no significant effects of embryo quality (P = 0.31) or technician (P = 0.12) on pregnancy rates. The mean interval from DIB removal to estrus was shorter (P = 0.001) for recipients in the EB0 group (22.7 ± 1.0 h) than for those in the EB24 group (37.4 ± 1.2 h). Nevertheless, pregnancy rates did not differ (P = 0.14) between recipients seen in estrus (EB0: 21/38, 55.3%, and EB 24: 16/34, 47.1%) and those not seen in estrus but with a CL >256 mm2 on Day 16 (EB0: 10/11, 90.9%, and EB24 4/9, 44.4%). It was concluded that the two treatments evaluated are equally efficacious for synchronizing Bos taurus × Bos indicus recipients. Furthermore, the use of EB at device removal could reduce the number of trips through the chute without affecting pregnancy rates. Research was supported by Syntex S.A., Estancia El Mangrullo S.A., and Agencia Cordoba Ciencia S.E.

39 EFFECT OF TIME OF FIXED-TIME AI ON PREGNANCY RATES IN ZEBU CROSS-BRED BEEF HEIFERS TREATED WITH PROGESTERONE RELEASING DEVICES AND ESTRADIOL CYPIONATE

2010 ◽  
Vol 22 (1) ◽  
pp. 177 ◽  
Author(s):  
M. Ramos ◽  
L. Cutaia ◽  
P. Chesta ◽  
G. A. Bó
Keyword(s):  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Beef Heifers ◽  
Treatment Groups ◽  
Condition Score ◽  
To Receive

Two experiments were designed to evaluate the effect of the timing of fixed-time AI (FTAI) in relation to the removal of an intravaginal progesterone-releasing device (1 g of progesterone, DIB, Syntex SA, Buenos Aires, Argentina) on pregnancy rates in Bos indicus × Bos taurus cross-bred heifers. In experiment 1, 285 Bonsmara × zebu cross-bred heifers, between 18 and 24 months of age and with a body condition score (BCS) between 3.0 and 3.5 (1-5 scale) were used. On the day of initiation of treatment (Day 0), the heifers’ ovaries were palpated (92% of them had a CL) and they received a new DIB plus 2 mg of estradiol benzoate (EB; Syntex SA) and 250 μg of cloprostenol (Ciclase DL, Syntex SA). On Day 8, DIB devices were removed and all heifers received 250 μg of Ciclase plus 0.5 mg of estradiol cypionate (ECP; Cipiosyn, Syntex SA). At that time the heifers were randomly divided to receive FTAI between 48 to 49 h, 53 to 54 h, or 58 to 59 h after DIB removal. The heifers underwent FTAI with semen from 4 bulls by 2 inseminators. In experiment 2, 260 heifers from the same group as those used in experiment 1 (87% with a CL) were treated exactly as those in experiment 1, except that previously used DIB was inserted on Day 0. Pregnancy diagnosis was performed 30 days post-fixed-time AI by ultrasonography. The data were analyzed by logistic regression, taking into account the effect of time of FTAI, semen, and inseminator on pregnancy rates. In experiment 1, pregnancy rates were lower (P = 0.04) in the heifers undergoing FTAI between 48 and 49 h after DIB removal (46/95, 48.4%) than those undergoing FTAI 53 to 54 h (61/99, 61.6%) or 58 to 60 h (57/91, 62.6%) after DIB removal. However, no differences in pregnancy rates were found (P = 0.72) in experiment 2 between the 3 treatment groups, with 39/91 (42.9%) for the 48 to 49 h group, 45/89 (50.6%) for the 53 to 54 h group, and 35/89 (43.8%) for the 58 to 59 h group. There was no effect of the semen or inseminator (P > 0.2) in either experiment. We conclude that when Bos indicus × Bos taurus beef heifers are synchronized with new DIB devices and ECP, higher pregnancy rates are obtained in heifers undergoing FTAI late (between 53 to 60 h after DIB removal) than in those undergoing FTAI early (48 to 49 h after DIB removal). However, time of insemination does not apparently affect pregnancy rates when Bos indicus × Bos taurus beef heifers are synchronized with previously used DIB devices and ECP.

20 PREGNANCY RATES IN SUCKLED BEEF COWS SYNCHRONIZED WITH PROGESTERONE INTRAVAGINAL DEVICES AND RECEIVING eCG FOURTEEN DAYS AFTER BREEDING

2010 ◽  
Vol 22 (1) ◽  
pp. 168 ◽  
Author(s):  
L. Cutaia ◽  
M. Ramos ◽  
P. Chesta ◽  
G. A. Bó
Keyword(s):  
Body Condition ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Treatment Groups ◽  
Condition Score

An experiment was designed to test the following hypotheses: (1) the administration of 400IU of eCG (Novormón, Syntex SA, Buenos Aires, Argentina) 14 days after fixed-time artificial insemination (FTAI) increases pregnancy rates in cross-breed Zebu × Bonsmara suckled cows synchronized with progesterone releasing devices (DIB 0.5, 0.5 g of progesterone, Syntex SA); and (2) pregnancy rates in cows treated with estradiol or GnRH, combined with a DIB device, are not different. Primiparous suckled cows (n = 260), between 60 and 120 days postpartum (DPP) and with a body condition score between 2.0 and 2.5 (1 to 5 scale) were used. At the beginning of the experiment (Day 0), all cows were examined by rectal palpation (only 11% had a CL), received a DIB device, and were randomly allocated to 1 of 3 treatment groups. Cows in the E2 group received 2 mg of estradiol benzoate (EB) intramuscularly (i.m.) at DIB insertion and those in the two GnRH groups received 100 μg of gonadorelin (GDR Gonasyn, Syntex SA) at the same time. On Day 7, DIB devices were removed and all cows received 500 μg of cloprostenol (Ciclase DL, Syntex SA) plus 400 IU of eCG (i.m.). Cows in the E2 group received 0.5 mg of estradiol cypionate (ECP, Cipiosyn, Syntex SA) and underwent FTAI administered by one technician, using semen from 1 of 2 bulls between 52 and 56 h later. Cows in the GnRH groups received a dose of 100 μg of gonadorelin (full GnRH group) or 50 μg of gonadorelin (1/2 GnRH group) at the time of the FTAI (52 to 56 h after DIB removal). Fourteen days after FTAI cows in the 3 groups were further subdivided (3 × 2 factorial) to receive or not 400 IU of eCG i.m. Pregnancy was determined 30 days after FTAI using real-time ultrasonography. Data were analyzed by logistic regression, taking into account the effect of the synchronization treatment, eCG or noteCG, 14 days after FTAI and sire used. No differences in pregnancy rates were found (P = 0.16) among treatment groups (E2: 39/87, 43.7%, full GnRH: 38/92, 41.3%, and 1/2 GnRH: 25/81, 30.9%). However, there was a significant (P = 0.02) effect of eCG treatment 14 days after FTAI (eCG: 60/127, 47.2% v. no eCG: 41/133, 30.8%). Furthermore, bull A (77/155, 47.1%) resulted in higher pregnancy rate (P = 0.009) than bull B (28/107, 26.2%), but there were no significant interactions between bull and synchronization treatment or eCG administration 14 days after FTAI (P > 0.1). We conclude that the application of 400 IU of eCG 14 days after FTAI increases pregnancy rates in suckled Bos taurus × Bos indicus primiparous cows with fair to poor body condition score synchronized with progesterone releasing devices. Furthermore, the use of estradiol ester or GnRH at DIB insertion and removal did not affect pregnancy rates.

88 Impacts of Body Condition Score at Initiation of the Breeding Season on Reproductive Performance and Overall Productivity of Bos Taurus and B. Indicus Beef Cows

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 48-48
Author(s):  
Alice Brandão ◽  
Reinaldo F Cooke ◽  
G Cliff C Lamb ◽  
José Luiz Vasconcelos ◽  
Ky G Pohler
Keyword(s):  
Breeding Season ◽  
Body Condition ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Beef Cows ◽  
Pregnancy Rates ◽  
Condition Score ◽  
Pregnancy Status ◽  
Calving Rate ◽  
Natural Service

Abstract Responses of Bos taurus (BT) and B. indicus (BI) beef cows were compared according to their body condition score (BCS) at initiation of breeding season. Cows were assigned to an estrus synchronization + AI protocol and classified as BCS < 5.0 (LBCS, BT, n = 158; BI, n = 248) or BCS ≥ 5.0 (HBCS; BT, n = 173; BI, n = 122). Pregnancy status was verified via transrectal ultrasonography at 30 and 40 d after AI for BI and BT cows, respectively. All BT cows were exposed to natural service for 50 d (beginning 18 d after AI), and all non-pregnant BI cows were immediately exposed to natural service for 60 d. Pregnancy rates to AI did not differ (P ≥ 0.16) between HBCS or LBCS cows (60.9 vs. 53.5% for BT; 37.0 vs. 31.4% for BI; respectively). Pregnancy rates to natural service were greater (P = 0.03) in HBCS BT cows compared with LBCS (82.7 vs. 67.9%; respectively) but did not differ in BI cows (78.9 vs. 71.5%; respectively). Calving rate was greater (P < 0.03) in HBCS cows, which also calved earlier (P < 0.01) compared with LBCS (93.7 vs. 85.1% in BT, 83.6 vs. 73.3% in BI; respectively). HBCS Cows weaned more (P ≤ 0.05) calves compared with LBCS, which were older (P ≤ 0.04) (215 vs. 211 d in BT, 219 vs. 209 d in BI; respectively) and heavier (P ≤ 0.09) at weaning (258 vs. 252 kg in BT, 213 vs. 203 d in BI; respectively). Lastly, kg of calf weaned/cow was greater (P ≤ 0.03) in HBCS cows compared with LBCS (221 vs. 200 d in BT, 159 vs. 129 d in BI; respectively). Therefore, HBCS cows had greater reproductive efficiency and overall productivity, and this was observed in both BT and BI.

scholarly journals Supplementing organic-complexed or inorganic Co, Cu, Mn, and Zn to beef cows during gestation: physiological and productive response of cows and their offspring until weaning

2021 ◽  
Author(s):  
Kelsey M Harvey ◽  
Reinaldo F Cooke ◽  
Eduardo A Colombo ◽  
Bruna Rett ◽  
Osvaldo A de Sousa ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Milk Production ◽  
Treatment Effects ◽  
Muscle Development ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Beef Cows ◽  
Condition Score ◽  
To Receive

Abstract One hundred and ninety non-lactating, pregnant beef cows (¾ Bos taurus and ¼ Bos indicus; 138 multiparous and 52 primiparous) were assigned to this experiment at 117 ± 2.2 d of gestation (d 0). Cows were ranked by parity, pregnancy type (artificial insemination = 102, natural service = 88), body weight (BW) and body condition score (BCS), and assigned to receive a supplement containing: 1) sulfate sources of Cu, Co, Mn, and Zn (INR; n = 95) or 2) an organic complexed source of Cu, Mn, Co, and Zn (AAC; Availa®4; Zinpro Corporation, Eden Prairie, MN; n = 95). The INR and AAC provided the same daily amount of Cu, Co, Mn, and Zn, based on 7 g of the AAC source. From d 0 to calving, cows were maintained in a single pasture and were segregated three times weekly into 1 of 24 individual feeding pens to receive treatments. Cow BW and BCS were recorded on d -30, d 97, upon calving, and at weaning (d 367). Milk production was estimated at 42 ± 0.5 d postpartum via weigh-suckle-weigh (WSW). Liver biopsies were performed in 30 cows/treatment on d -30, 97, upon calving, and the day after WSW. Calf BW was recorded at birth and weaning. Liver and longissimus muscle (LM) biopsies were performed in 30 calves/treatment upon calving and 24 h later, the day after WSW, and at weaning. No treatment effects were detected (P ≥ 0.49) for cow BCS during gestation, despite AAC cows having greater (P = 0.04) BW on d 97. Liver Co concentrations were greater (P < 0.01) for AAC compared to INR cows, and liver concentrations of Cu were greater (P = 0.02) for INR compared to AAC cows on d 97. Upon calving, INR cows had greater (P ≤ 0.01) liver Cu and Zn concentrations compared to AAC cows. No other treatment differences were noted (P ≥ 0.17) for cow and calf liver trace mineral concentrations. Cows receiving AAC had greater (P = 0.04) hepatic mRNA expression of metallothionein 1A at calving, and their calves had greater (P = 0.04) hepatic mRNA expression of superoxide dismutase at weaning. Milk production did not differ between AAC and INR cows (P = 0.70). No treatment effects were detected (P ≥ 0.29) for mRNA expression of LM genes associated with adipogenic or muscle development activities in calves at birth and weaning. Calf birth and weaning BW also did not differ (P ≥ 0.19) between treatments. In summary, supplementing Co, Cu, Zn, and Mn as organic complexed or sulfate sources to beef cows during the last 5 mo of gestation yielded similar cow-calf productive responses until weaning.

scholarly journals 14 EFFECT OF TEMPORARY WEANING AND ECG TREATMENT ON PREGNANCY RATES IN POSTPARTUM BOS INDICUS COWS FOLLOWING TREATMENT WITH PROGESTERONE VAGINAL DEVICES AND ESTRADIOL BENZOATE

2005 ◽  
Vol 17 (2) ◽  
pp. 157
Author(s):  
L. Cutaia ◽  
E. Balla ◽  
C. Rizzi ◽  
L. Peres ◽  
G.A. Bo
Keyword(s):  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Medium Size ◽  
Ovarian Activity ◽  
Condition Score ◽  
Rectal Palpation

Although treatments with progesterone (P4)-releasing devices and estradiol benzoate (EB) have been extensively used in fixed-time AI (FTAI) programs in beef cattle, pregnancy rates in postpartum Bos indicus cows kept on pasture often have been lower than expected because of poor body condition score (BCS) and a high incidence of anestrus. Temporary weaning and eCG treatment have been shown to increase pregnancy rates in suckled beef cows. Therefore, this experiment was designed to compare the effect of eCG treatment and temporary weaning on pregnancy rates in postpartum cows in fair to poor BCS. Lactating multiparous crossbred Bos indicus cows (n = 393), 60 to 90 d postpartum with a BCS 2.0 (1 to 5 scale) were randomly allocated to 1 of 4 treatment groups, in a 2 × 2 factorial design. At the beginning of the experiment (Day 0), all cows received a P4 intravaginal device (DIB, Syntex, Argentina) and 2 mg EB i.m. (Syntex). On Day 8, DIB devices were removed and all cows received 150 μg D (+) cloprostenol i.m. (Ciclase, Syntex) and were randomly divided to receive 400 IU eCG (Novormon 5000, Syntex) or no treatment at the same time. Furthermore, half of the cows in each treatment group have their calves weaned temporarily, from the time of DIB removal until the end of the FTAI, whereas the other half remained with their calves all the time. Finally, all cows received 1 mg EB i.m. on Day 9 and were FTAI 52 to 56 h after DIB removal. Cows were examined by ultrasonography 42 d after FTAI to determine pregnancy status. Data were analyzed by logistic regression and the effects of treatment and semen used were considered in the model. Ovarian activity was estimated by rectal palpation on Day 0: there were 72/393 (18.2%) cows with a CL, 140/393 (35.6%) cows with palpable medium size follicles, and 181/393 (46.1%) cows with ovaries containing no detectable structures. There was no effect of ovarian status at the time of treatment (P = 0.91) or semen (P = 0.91) on pregnancy rates. Treatment with eCG tended (P = 0.08) to increase pregnancy rates (94/192, 48.9% and 79/201, 39.3% for cows treated or not treated with eCG, respectively). However, there was no effect of weaning or eCG by weaning interaction (P = 0.98) on pregnancy rates (86/191, 45.1% and 87/202, 43.1% for cows that have their calves weaned or not weaned for 56 h, respectively). Results confirm those of previous studies that demonstrated that the use of eCG in a P4/EB/FTAI program improved pregnancy rates in postpartum Bos indicus crossbred cows that were in fair-to-poor BCS. However, no improvement in pregnancy rates was observed after temporary weaning in the present study. Research was supported by Syntex S.A., Estancia El Mangrullo S.A., and Agencia Cordoba Ciencia S.E.

Setting the Cycle for Reproductive Success with a Pre-synchronization Program in Postpartum Beef Cows

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 23-23
Author(s):  
Macy M Massengill ◽  
Thiago V Silva ◽  
Daniel H Poole
Keyword(s):  
Body Condition Score ◽  
Statistical Significance ◽  
Beef Cows ◽  
Pregnancy Rates ◽  
Estrous Synchronization ◽  
Condition Score ◽  
Pregnancy Status ◽  
Timed Artificial Insemination ◽  
To Receive ◽  
Synchronization Protocol

Abstract Previous experiments from our laboratory determined that postpartum beef cows with a functional corpus luteum (CL) at the start of an estrous synchronization protocol have greater pregnancy success to FTAI than those that did not have a CL. Therefore, it was hypothesized that incorporation a presynchronization program prior to FTAI in postpartum cows will improve pregnancy rates. The goal of this project was to compare the effectiveness of a presynchronization program prior to the 7-d CO-Synch+CIDR fixed timed artificial insemination (FTAI) protocol in postpartum cows. Postpartum cows (n = 310) at two locations were randomly assigned to receive either the presynchronization program, consisting of 2 PGF2α injections 14 d apart and 11 d prior to the start of the 7-d CO-Synch+CIDR protocol, or the 7-d CO-Synch + CIDR protocol. Following estrus synchronization, all cows were inseminated by trained technicians using semen from bulls of known fertility. Pregnancy status to FTAI was assessed using ultrasonographic diagnosis at 30 d post insemination. Data were analyzed using a MIXED procedure of SAS and examined for effects of inseminator, body condition score (BCS), and location and age. Statistical significance was determined at P < 0.05 and a tendency at 0.05< P < 0.10. Age, BCS, and location did not affect (P > 0.05) pregnancy rates. Cows exposed to the pre-synchronization program tended to have greater AI pregnancy rates compared to cows that did not receive the pre-synchronization program (57.7±5.3 vs. 46.9±5.3%, respectively; P = 0.0687). AI pregnancy rates were greater in cows with >1 ng/mL P4 compared to cows with < 1 ng/mL P4 at the start of FTAI (77.7±7 vs. 55.7±6.2%, respectively; P = 0.0204). Furthermore, AI pregnancy rates were decreased by inseminator C (26.1±7.4) compared to the other inseminators this breeding season (A-54.4±7.9; B- 62.3±5.3; D-57.8±7.2; and E -51.1±5.2; P = 0.0026). Based on these data, incorporating a pre-synchronization program to ensure the presence of a CL at the start of a FTAI protocol may improve pregnancy success in postpartum beef cows.

207 INFLUENCE OF THE INTERVAL BETWEEN THAWING TO TRANSFER ON PREGNANCY RATES OF FROZEN - THAWED DIRECT-TRANSFER EMBRYOS

2007 ◽  
Vol 19 (1) ◽  
pp. 220
Author(s):  
G. A. Bo ◽  
L. C. Peres ◽  
D. Pincinato ◽  
M. de la Rey ◽  
R. Tribulo
Keyword(s):  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Direct Transfer ◽  
Condition Score ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

An experiment was designed to evaluate the effect of the interval between thawing to deposition of the embryo into the uterine horn on pregnancy rates of in vivo-produced frozen–thawed embryos in 1.5 M ethylene glycol (direct transfer). Data were collected from 1122 embryo transfers performed in the same farm (Estancia El Mangrullo, Lavalle, Santiago del Estero, Argentina) during the spring and summer of 2004/05 and 2005/06 (6 replicates, ambient temperature between 20 and 40�C). Recipients used in all replicates were non-lactating, cycling, multiparous Bos taurus � Bos indicus crossbred cows with body condition score between 3 and 4 (1 to 5 scale) that were synchronized using fixed-time embryo transfer protocols. Briefly, the synchronization treatments consisted of the insertion of a Crestar ear implant (Intervet, Sao Paulo, Brazil) or a progesterone-releasing device (DIB; Syntex SA, Buenos Aires, Argentina), plus 2 mg of estradiol benzoate (EB; Syntex) intramuscularly (IM) on Day 0, and 400 IU of eCG (Folligon 5000; Intervet, or Novormon 5000; Syntex) IM plus 150 �g d-cloprostenol IM (Preloban; Intervet, or Ciclase; Syntex) on Day 5. Progestin devices were removed on Day 8 and all cows received 1 mg of EB IM on Day 9. All cows were examined by ultrasonography on Day 16 and those with a luteal area >76 mm2 (by calculating the area of the CL minus the area of the cavity) received, on Day 17, frozen–thawed embryos by nonsurgical transfer. All embryos were Grade 1, and all were frozen in 1.5 M ethylene glycol at the Embryo Plus Laboratory (Brits, South Africa). After being stored in liquid nitrogen, the embryos were plunged directly (no air thawing) in a 30�C water bath for 30 s, and then transferred to the recipient cows by either one of two technicians. Based on the interval between thawing and transfer, the transfers were classified as being in one of 3 groups: Group 1: <3 min; Group 2: 3 to 6 min; and Group 3: 6 to 16 min. The main reason for delayed transfers beyond 6 min was the replacement of one recipient for another because of difficulty in threading the cervix (1% of the total transfers) or a recipient falling down into the chute or with very bad disposition and behavior. Pregnancy was determined by ultrasonography 28 to 35 days after fixed-time embryo transfer, and data were analyzed by logistic regression. There were no effects of replicate, technician, CL area, recipient body condition score, embryo stage, and time from thawing to transfer on pregnancy rates. Pregnancy rates in the 3 thawing to transfer intervals were: Group 1: 215/385, 55.8%; Group 2: 372/655, 56.8%; Group 3: 42/82, 51.2%; P > 0.6. These results may be interpreted to suggest that there is no significant effect of time from thawing to transfer (up to 16 min) in direct transfer embryos using Bos taurus � Bos indicus recipients transferred at a fixed time.

20 ADMINISTRATION OF FOLLICLE-STIMULATING HORMONE AT CIDR REMOVAL DOES NOT AFFECT THE PREGNANCY RATE IN A CIDR-BASED, COSYNCH PROTOCOL IN LACTATING BEEF COWS

2008 ◽  
Vol 20 (1) ◽  
pp. 90 ◽  
Author(s):  
J. Small ◽  
F. Dias ◽  
L. Pfeifer ◽  
K. Lightfoot ◽  
M. Colazo ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Pregnancy Rate ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Animal Health ◽  
Beef Cows ◽  
Pregnancy Rates ◽  
Condition Score ◽  
Secondary Objective ◽  
Pfizer Animal Health

In previous studies, giving eCG at CIDR removal significantly increased the pregnancy rate after timed-AI in beef cows. However, eCG is not universally available. Therefore, we tested the hypothesis that giving pFSH at CIDR removal might improve the pregnancy rate in a CIDR-based, Cosynch protocol in postpartum, suckled Bos taurus beef cows; a secondary objective was to compare pregnancy rates when GnRH v. pLH was used to synchronize wave emergence and ovulation. This work was conducted as two experiments (separate locations). All cows were given a CIDR (containing 1.9 g progesterone; Pfizer Animal Health, Montreal, QC, Canada) on Day 0 (without regard to stage of estrous cycle or cyclicity). On Day 7, CIDRs were removed, all cows were concurrently given 25 mg PGF (Lutalyse; Pfizer Animal Health, Groton, CT, USA), half were given 20 mg pFSH (Folltropin-V; Bioniche Animal Health, Belleville, ON, Canada), and all cows were timed-AI 54 h later (Day 9). In Experiment 1, 240 cows [94 � 10.8 days postpartum; body condition score (BCS: 1 = emaciated, 9 = obese; mean � SD): 5.8 � 0.4] were used; at CIDR insertion and AI, cows were allocated to receive either 100 µg GnRH (n = 160; Cystorelin, Merial Canada Inc., Victoriaville, QC, Canada) or 12.5 mg pLH (n = 80; Lutropin-V, Bioniche Animal Health). In Experiment 2, 109 cows (59.2 � 19.5 days postpartum; BCS: 5.6 � 1.1) were used; all received 100 µg GnRH (Cystorelin) at CIDR insertion and AI. In Experiment 1, three cows that lost their CIDR were excluded; pregnancy rates after timed-AI (logistic regression, backward selection: parity, pFSH, synchronizing treatment, and their interactions) were not different between cows given pFSH v. control cows (64.7 v. 65.2%; P > 0.80), nor between cows given GnRH v. pLH (62.7 v. 69.6%; P = 0.91). However, there was an interaction (P < 0.04) between parity and the synchronizing treatment; in primiparous cows, pregnancy rates were significantly lower in those given GnRH v. pLH (59.3 v. 83.3%). In Experiment 2, pregnancy rates after timed-AI (logistic regression, backward selection: parity, pFSH, and their interaction) were not different between cows given pFSH v. control cows (38.2 v. 42.6%; P > 0.6). In conclusion, our hypothesis was not supported; giving pFSH at CIDR removal did not significantly improve the pregnancy rate in a CIDR-based, Cosynch protocol in postpartum, suckled Bos taurus beef cows.

7 INFLUENCE OF ESTRUS EXPRESSION AND TREATMENT WITH GnRH ON PREGNANCY RATES IN BEEF CATTLE SYNCHRONIZED WITH PROGESTERONE DEVICES AND ESTRADIOL AND INSEMINATED AT A FIXED-TIME

2017 ◽  
Vol 29 (1) ◽  
pp. 111 ◽  
Author(s):  
G. A. Bó ◽  
A. Cedeño ◽  
A. Tribulo ◽  
S. Andrada ◽  
R. Tribulo ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Beef Cattle ◽  
Body Condition Score ◽  
Fixed Time ◽  
Beef Cows ◽  
Pregnancy Rates ◽  
Device Removal ◽  
Oestradiol Benzoate ◽  
Condition Score ◽  
To Receive

We have previously shown that expression of oestrus significantly influences the time of ovulation and pregnancy rates to timed-AI (P/TAI) in beef cattle synchronized with progesterone devices and oestradiol (Bó et al. 2016 Theriogenology 86, 388–396). Furthermore, delaying TAI from 48 h to 54–58 h after device removal improved P/TAI of those animals not showing oestrus by 48 h. The objective of the present study was to determine whether the administration of GnRH to those animals not showing oestrus by 48 h after device removal had an impact on P/TAI. Non-lactating and suckled beef cows and heifers (Bonsmara, Brangus, and Braford; n = 868), with a corpus luteum (CL) or a follicle ≥8 mm in diameter detected by ultrasonography (Mindray DP 30 Vet, 7.5 MHz, China) and body condition score 2 to 4 (1 to 5 scale) were synchronized in 3 replicates. On Day 0, animals received a progesterone device (DIB 0.5 g, Zoetis, Argentina) and 2 mg of oestradiol benzoate (Von Franken, Argentina). On Day 8, DIB were removed and cows received 0.150 mg of D+cloprostenol (Río de Janeiro, Argentina) plus 0.5 mg oestradiol cypionate (Cipiosyn, Zoetis). In addition, all cows were tail-painted on the sacrococcygeal area (CeloTest, Biotay, Argentina) at DIB removal and were observed to determine the expression of oestrus, according to the percentage of paint loss (PL) 48 h later. The animals that showed oestrus (PL > 30%) were recorded and TAI at that time while those with PL ≤ 30% (not in oestrus) were randomly allocated to receive 100 μg of gonadorelin (GnRH; Gonasyn, Zoetis) or no treatment and TAI between 54 to 58 h after DIB removal. Pregnancy was diagnosed by ultrasonography at 45 days after TAI, and data were analysed by logistic regression. There were no significant differences among replicates, inseminators, semen source or animal class (dry cows, suckled cows or heifers; P > 0.11). However, animals showing oestrus by 48 h after DIB removal had higher P/TAI (371/666; 55.7; P < 0.04) than those that did not show oestrus (96/202; 47.5%). Within animals not showing oestrus at 48 h, those that were in oestrus by the TAI at 54 to 58 h had higher P/TAI (71/124, 57.3%; P < 0.01) than those that did not show oestrus within that period (27/78; 34.6%). In addition, P/TAI was higher (P < 0.01) in animals that received GnRH (66/113; 58.4%) than in those that did not receive GnRH (30/89; 33.7%). In conclusion, the expression of oestrus significantly affected P/TAI in beef cattle synchronized with progesterone devices and oestradiol. Furthermore, treatment of animals not showing oestrus by 48 h after DIB removal with GnRH resulted in improved P/TAI performed 6 to 10 h later.

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