scholarly journals Barrier protective effects of 2,4,6-trihydroxy-3-geranyl acetophenone on lipopolysaccharides-stimulated inflammatory responses in human umbilical vein endothelial cells

2016 ◽  
Vol 192 ◽  
pp. 248-255 ◽  
Author(s):  
Yi Joong Chong ◽  
Nazmi Firdaus Musa ◽  
Chean Hui Ng ◽  
Khozirah Shaari ◽  
Daud Ahmad Israf ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Inflammatory Responses ◽  
Umbilical Vein ◽  
Protective Effects ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

scholarly journals Protective effects of hyperoside against H2O2-induced apoptosis in human umbilical vein endothelial cells

2016 ◽  
Vol 14 (1) ◽  
pp. 399-405 ◽  
Author(s):  
XU-LIANG HAO ◽  
YA KANG ◽  
JIAN-KUAN LI ◽  
QING-SHAN LI ◽  
EN-LI LIU ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Protective Effects ◽  
Human Umbilical Vein ◽  
Induced Apoptosis ◽  
Umbilical Vein Endothelial Cells

Factor Xa inhibits HMGB1-induced septic responses in human umbilical vein endothelial cells and in mice

2014 ◽  
Vol 112 (10) ◽  
pp. 757-769 ◽  
Author(s):  
Wonhwa Lee ◽  
Sae-Kwang Ku ◽  
Jong-Sup Bae
Keyword(s):  
Endothelial Cells ◽  
Nuclear Dna ◽  
Inflammatory Responses ◽  
Umbilical Vein ◽  
Cecal Ligation ◽  
Post Treatment ◽  
Coagulation Cascade ◽  
Factor X ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

SummaryNuclear DNA-binding protein high mobility group box 1 (HMGB1) acts as a late mediator of severe vascular inflammatory conditions, such as sepsis. Activated factor X (FXa) is an important player in the coagulation cascade responsible for thrombin generation, and it influences cell signalling in various cell types by activating protease-activated receptors (PARs). However, the effect of FXa on HMGB1-induced inflammatory response has not been studied. First, we addressed this issue by monitoring the effects of post-treatment with FXa on lipopolysaccharide (LPS)- and cecal ligation and puncture (CLP)-mediated release of HMGB1 and HMGB1-mediated regulation of pro-inflammatory responses in human umbilical vein endothelial cells (HUVECs) and septic mice. Post-treatment with FXa was found to suppress LPS-mediated release of HMGB1 and HMGB1-mediated cytoskeletal rearrangements. FXa also inhibited HMGB1-mediated hyperpermeability and leukocyte migration in septic mice. In addition, FXa inhibited the production of tumour necrosis factor-α and interleukin (IL)-1β. FXa also facilitated the downregulation of CLP-induced release of HMGB1, production of IL-6, and mortality. Collectively, these results suggest that FXa may be regarded as a candidate therapeutic agent for treating vascular inflammatory diseases by inhibiting the HMGB1 signalling pathway.

Protective effects of S ‐carvedilol on doxorubicin‐induced damages to human umbilical vein endothelial cells and rats

2019 ◽  
Vol 39 (8) ◽  
pp. 1233-1244 ◽  
Author(s):  
Ting Wu ◽  
Haixin Li ◽  
Qunsheng Lan ◽  
Ze‐an Zhao ◽  
Ying Cao ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Protective Effects ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

scholarly journals The protective effect of thymoquinone on tert-butylhydroquinone induced cytotoxicity in human umbilical vein endothelial cells

2019 ◽  
Vol 8 (6) ◽  
pp. 1050-1056
Author(s):  
Zahra Karimi ◽  
Maryam Ghaffari ◽  
Jafar Ezzati Nazhad Dolatabadi ◽  
Parvin Dehghan
Keyword(s):  
Endothelial Cells ◽  
Protective Effect ◽  
Food Industry ◽  
Umbilical Vein ◽  
Protective Agent ◽  
Protective Effects ◽  
Oxidative Rancidity ◽  
Dapi Staining ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Abstract 2-tert-Butyl-4-hydroquinone (TBHQ) is used for inhibition of oxidative rancidity in the food industry. However, this antioxidant can stimulate cytotoxicity in human umbilical vein endothelial cells (HUVECs). Thus, potential protective effects of thymoquinone (TQ) against TBHQ-induced cytotoxicity were investigated. Cytotoxicity was evaluated via MTT, flow cytometry, DAPI staining and DNA fragmentation methods. The obtained results revealed that treatment of HUVECs with TQ enhanced the cell viability rate and it had potential to reduce the cytotoxicity effect of TBHQ in cells. Also, in a combined regime of TQ and TBHQ, apoptosis was reduced compared to the cells treated with TBHQ (p < 0.05). Similarly, TQ had a protective effect on DNA and chromatin fragmentation of the cells treated with TBHQ. Finally, it can be concluded that TQ could be used as a protective agent against cytotoxicity induced by TBHQ in HUVECs.

Sign in / Sign up

Export Citation Format

Share Document