In vitro screening of six anthelmintic plant products against larval Haemonchus contortus with a modified methyl-thiazolyl-tetrazolium reduction assay

2006 ◽  
Vol 108 (1) ◽  
pp. 85-89 ◽  
Author(s):  
P. Hördegen ◽  
J. Cabaret ◽  
H. Hertzberg ◽  
W. Langhans ◽  
V. Maurer
Keyword(s):  
Haemonchus Contortus ◽  
Methyl Thiazolyl Tetrazolium ◽  
In Vitro Screening ◽  
Plant Products ◽  
Reduction Assay ◽  
Tetrazolium Reduction

Comparison of motility, recovery, and methyl-thiazolyl-tetrazolium reduction assays for use in screening plant products for anthelmintic activity

2009 ◽  
Vol 105 (5) ◽  
pp. 1339-1343 ◽  
Author(s):  
Robert A. Smith ◽  
Laura Pontiggia ◽  
Carrie Waterman ◽  
Meghan Lichtenwalner ◽  
Jared Wasserman
Keyword(s):  
Anthelmintic Activity ◽  
Methyl Thiazolyl Tetrazolium ◽  
Plant Products ◽  
Tetrazolium Reduction

scholarly journals In vitro screening of various forages for anthelmintic activity on Haemonchus contortus eggs

2010 ◽  
Vol 1 (1) ◽  
pp. 113-113 ◽  
Author(s):  
J Daryatmo ◽  
H Hartadi ◽  
E.R Orskov ◽  
K Adiwimarta ◽  
W Nurcahyo
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
In Vitro Screening

In vitro screening of plant extract on Haemonchus contortus and Rhipicephalus (Boophilus) microplus

2020 ◽  
Vol 32 (3) ◽  
pp. 269-278
Author(s):  
Alessandro P. Minho ◽  
Luciana F. Domingues ◽  
Yousmel A. Gainza ◽  
Amanda Figueiredo ◽  
Aline A. Boligon ◽  
...  
Keyword(s):  
Plant Extract ◽  
Haemonchus Contortus ◽  
Boophilus Microplus ◽  
In Vitro Screening

In vitro screening of Haemonchus contortus third stage larvae for ivermectin resistance

1996 ◽  
Vol 61 (1-2) ◽  
pp. 73-80 ◽  
Author(s):  
J.A. d'Assonville ◽  
E. Janovsky ◽  
A. Verster
Keyword(s):  
Haemonchus Contortus ◽  
In Vitro Screening ◽  
Third Stage

scholarly journals In Vitro Screening of Antifungal Potency of Plant Products against Rhizoctonia solani

2016 ◽  
Vol 5 (7) ◽  
pp. 633-637
Author(s):  
Sandeep Chaudhary ◽  
Akhilesh Kumar Gupta ◽  
C.O. Samuel ◽  
P.P. Upadhyaya
Keyword(s):  
Rhizoctonia Solani ◽  
In Vitro Screening ◽  
Plant Products

Effect of Jatropha curcas Latex on L3 Haemonchus contortus Larval Motility

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Noorzaid Muhamad ◽  
Syahirah Sazeli ◽  
Resni Mona ◽  
Jannathul Firdous
Keyword(s):  
Larvicidal Activity ◽  
Jatropha Curcas ◽  
Haemonchus Contortus ◽  
Anthelmintic Resistance ◽  
Small Ruminants ◽  
Gastrointestinal Nematodes ◽  
Dose Dependent ◽  
Plants Extract

The anthelmintic resistance has limited the control of gastrointestinal nematodes of small ruminants and thus has awakened interest in the study of plants extract as a source of anthelmintics. These experiments were carried out to evaluate the in vitro efficacy of Jatrophacurcas latex extract against Haemonchuscontortus larval motility. To evaluate the larvicidal activity, H.contortus L3 were incubated with the extracts with varying concentration of 5 mg/mL, 10 mg/mL, 15 mg/mL and 20 mg/mL at 27°C for 48, 72 and 96 hrs. The results were subjected to the Kruskal-Wallis test (P less than 0.05). The extracts showed dose-dependent larvicidal effects. These results suggest that J.curcas can be used to control gastrointestinal nematodes of small ruminants.

In Vitro Screening of Azalea for Resistance to Azalea Lace Bug

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 506b-506
Author(s):  
Carol D. Robacker ◽  
S.K. Braman
Keyword(s):  
Leaf Damage ◽  
In Vitro Screening ◽  
Screening Assay ◽  
Delaware Valley ◽  
In Vitro Techniques ◽  
Culture Tube ◽  
Lace Bug ◽  
Lace Bugs ◽  
Laboratory Bioassays

Azalea lace bug (Stephanitis pyrioides) is the most serious pest on azalea. Results of laboratory bioassays and field evaluations of 17 deciduous azalea taxa have identified three resistant taxa: R. canescens, R. periclymenoides, and R. prunifolium. Highly susceptible taxa are `Buttercup', `My Mary', R. oblongifolium, and the evergreen cultivar `Delaware Valley White'. To determine whether in vitro techniques would have potential value in screening or selecting for resistance, or for the identification of morphological or chemical factors related to resistance, an in-vitro screening assay was developed. In-vitro shoot proliferation was obtained using the medium and procedures of Economou and Read (1984). Shoots used in the bioassays were grown in culture tubes. Two assays were developed: one for nymphs and one for adult lace bugs. To assay for resistance to nymphs, `Delaware Valley White' leaves containing lace bug eggs were disinfested with 70% alcohol and 20% commercial bleach, and incubated in sterile petri plates with moistened filter paper until the nymphs hatched. Five nymphs were placed in each culture tube, and cultures were incubated for about 2 weeks, or until adults were observed. To assay for resistance to adults, five female lace bugs were placed in each culture tube and allowed to feed for 5 days. Data collected on survival and leaf damage was generally supportive of laboratory bioassays and field results. Adult lace bugs had a low rate of survival on resistant taxa. Survival of nymphs was somewhat reduced on resistant taxa.

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