0699 In vitro screening of the anthelmintic efficacy of birdsfoot trefoil commercial varieties and cultivars against ovine Haemonchus contortus

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 334-335
Author(s):  
C. Barone ◽  
S. Ferguson ◽  
A. Zajac ◽  
R. Brown ◽  
J. Reed ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Birdsfoot Trefoil ◽  
In Vitro Screening ◽  
Anthelmintic Efficacy

scholarly journals In vitro nematicidal effect of Chenopodium ambrosioides and Castela tortuosa n-hexane extracts against Haemonchus contortus (Nematoda) and their anthelmintic effect in gerbils

2018 ◽  
Vol 93 (04) ◽  
pp. 434-439 ◽  
Author(s):  
A. Zamilpa ◽  
C. García-Alanís ◽  
M.E. López-Arellano ◽  
V.M. Hernández-Velázquez ◽  
M.G. Valladares-Cisneros ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Adult Stage ◽  
Chenopodium Ambrosioides ◽  
Anthelmintic Efficacy ◽  
Hexane Extracts ◽  
Parasitic Burden ◽  
The Individual ◽  
Anthelmintic Effect

AbstractThe in vitro nematicidal effect of Chenopodium ambrosioides and Castela tortuosa n-hexane extracts (E-Cham and E-Cato, respectively) on Haemonchus contortus infective larvae (L3) and the anthelmintic effect of these extracts against the pre-adult stage of the parasite in gerbils were evaluated using both individual and combined extracts. The in vitro confrontation between larvae and extracts was performed in 24-well micro-titration plates. The results were considered 24 and 72 h post confrontation. The in vivo nematicidal effect was examined using gerbils as a study model. The extracts from the two assessed plants were obtained through maceration using n-hexane as an organic agent. Gerbils artificially infected with H. contortus L3 were treated intraperitoneally with the corresponding extract either individually or in combination. The results showed that the highest individual lethal in vitro effect (96.3%) was obtained with the E-Cham extract at 72 h post confrontation at 40 mg/ml, followed by E-Cato (78.9%) at 20 mg/ml after 72 h. The highest combined effect (98.7%) was obtained after 72 h at 40 mg/ml. The in vivo assay showed that the individual administration of the E-Cato and E-Cham extracts reduced the parasitic burden in gerbils by 27.1% and 45.8%, respectively. Furthermore, the anthelmintic efficacy increased to 57.3% when both extracts were administered in combination. The results of the present study show an important combined nematicidal effect of the two plant extracts assessed against L3 in gerbils.

In vitro screening of 51 birdsfoot trefoil (Lotus corniculatus L.; Fabaceae) strains for anti-parasitic effects against Haemonchus contortus

Parasitology ◽  
2019 ◽  
Vol 146 (6) ◽  
pp. 828-836 ◽  
Author(s):  
Carly D. Barone ◽  
Anne M. Zajac ◽  
Sarah M. Ferguson ◽  
Rebecca N. Brown ◽  
Jess D. Reed ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Small Ruminants ◽  
Secondary Compounds ◽  
Lotus Corniculatus ◽  
Birdsfoot Trefoil ◽  
Aqueous Extracts ◽  
Egg Hatching ◽  
Parasitic Activity ◽  
Parasitic Effects

AbstractSecondary plant compounds have shown bioactivity against multi-drug resistant Haemonchus contortus in small ruminants. This study screened 51 strains of birdsfoot trefoil (BFT, Lotus corniculatus) crude aqueous extracts (BFT-AqE) for anti-parasitic activity in vitro against egg hatching, and of those 51 strains, 13 were selected for further testing of motility of first (L1) and third stage (L3) larvae, and exsheathment of L3. Proanthocyanidin content ranged between 1.4 and 63.8 mg PAC g−1 powder across the 51 BFT strains. When tested against egg hatching, 21 of the 51 aqueous extracts had an EC50 of 1–2 mg powder mL−1, 70% of the strains were >90% efficacious at 6 mg powder mL−1 and 11 of the strains were 100% efficacious at 3 mg powder mL−1 BFT-AqE. Across the 13 strains tested against L3, efficacy ranged from 0 to 75% exsheathment inhibition, and 17 to 92% L3 motility inhibition at a concentration of 25 mg powder mL−1 BFT-AqE. There was no correlation between the PAC content of BFT powders and the anti-parasitic activity of aqueous extracts, therefore other secondary compounds may have contributed to the observed anti-parasitic effects. Further testing of BFT using bioactivity-driven fractionation and screening of BFT populations for the identified anti-parasitic compounds is needed.

scholarly journals In vitro screening of various forages for anthelmintic activity on Haemonchus contortus eggs

2010 ◽  
Vol 1 (1) ◽  
pp. 113-113 ◽  
Author(s):  
J Daryatmo ◽  
H Hartadi ◽  
E.R Orskov ◽  
K Adiwimarta ◽  
W Nurcahyo
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
In Vitro Screening

In-vitro Anthelmintic Efficacy of Some Kashmiri Medicinal Plants against Haemonchus contortus

2015 ◽  
Vol 5 (5) ◽  
pp. 73 ◽  
Author(s):  
Shahjahan Alam ◽  
Tarun Sarkar ◽  
Irfan Shah ◽  
Imtiyaz Reshi ◽  
Mazar Shah
Keyword(s):  
Medicinal Plants ◽  
Haemonchus Contortus ◽  
Anthelmintic Efficacy

In vitro screening of plant extract on Haemonchus contortus and Rhipicephalus (Boophilus) microplus

2020 ◽  
Vol 32 (3) ◽  
pp. 269-278
Author(s):  
Alessandro P. Minho ◽  
Luciana F. Domingues ◽  
Yousmel A. Gainza ◽  
Amanda Figueiredo ◽  
Aline A. Boligon ◽  
...  
Keyword(s):  
Plant Extract ◽  
Haemonchus Contortus ◽  
Boophilus Microplus ◽  
In Vitro Screening

In vitro screening of Haemonchus contortus third stage larvae for ivermectin resistance

1996 ◽  
Vol 61 (1-2) ◽  
pp. 73-80 ◽  
Author(s):  
J.A. d'Assonville ◽  
E. Janovsky ◽  
A. Verster
Keyword(s):  
Haemonchus Contortus ◽  
In Vitro Screening ◽  
Third Stage

scholarly journals IN VITRO ANTHELMINTIC ACTIVITY OF ABUTILON THEOPHRASTI MEDIK. (MALVACEAE) AGAINST EGGS AND L3 LARVAE OF HAEMONCHUS CONTORTUS

2019 ◽  
pp. 205-207
Author(s):  
MUSHEERUL HASSAN ◽  
MIR RA ◽  
TRIPTI JOSHI ◽  
FIRDOUS AHMAD YATOO ◽  
HUMA HABIB
Keyword(s):  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Abutilon Theophrasti ◽  
Aqueous Extracts ◽  
Egg Hatch ◽  
Anthelmintic Efficacy ◽  
Dependent Inhibition ◽  
Hexane Extracts ◽  
Extraction Solvents

Objective: The present study was carried out to assess the in vitro anthelmintic activity of Abutilon theophrasti stem extracts. Methods: Simple maceration was employed for extraction. Solvents such as methanol, water, and hexane were used. Egg hatch test (EHT) and larval motility test were employed to check the anthelmintic activity of crude extracts. Concentrations of 500, 250, 125, 62.5, and 31.25 mg/ml were made. Levamisole and distilled water served as control, respectively. Results: All selected extracts displayed concentration-dependent inhibition except aqueous extracts. At higher concentration (500 mg/ml), stem extracts (methanol, aqueous, and ethanol) showed 74.39%, 72.5 and 70.03% of efficacy in EHT, respectively (p≤0.05). Meanwhile, inhibition of larval motility was seen higher with inhibition percentage of 79.79, 75.65, and 71.28 by methanolic, aqueous, and hexane extracts, respectively (p≤ 0.05). Conclusion: The presents study suggests active principles having anthelmintic efficacy in Abutilon theopharsti stem.

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