Comparison of hepatitis C virus RNA detection in plasma, whole blood and peripheral blood mononuclear cells of patients with occult hepatitis C virus infection

2004 ◽  
Vol 31 (4) ◽  
pp. 312-313 ◽  
Author(s):  
Vicente Carreño ◽  
Inmaculada Castillo ◽  
Javier Bartolomé ◽  
Elena Rodríguez-Iñigo ◽  
Nuria Ortiz-Movilla ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Mononuclear Cells ◽  
Hepatitis C Virus Infection ◽  
Hepatitis C Virus Rna ◽  
Peripheral Blood Mononuclear ◽  
Rna Detection ◽  
Occult Hepatitis ◽  
Virus Rna ◽  
Occult Hepatitis C

Prevalence of Occult Hepatitis C Virus Infection among Egyptian Haemodialysis Patients

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Noha Abd EL-Razek El-Nakeeb ◽  
Moataz Mohamed El-Sayed ◽  
Tari Magdy Aziz George ◽  
Amira Mohamed Helmy Okasha El-Araishy
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cells ◽  
Mononuclear Cells ◽  
Hemodialysis Patients ◽  
Hcv Rna ◽  
Peripheral Blood Mononuclear ◽  
Occult Hepatitis ◽  
Blood Mononuclear Cells ◽  
Occult Hepatitis C

Abstract Background Hepatitis C Virus (HCV) infection is a global health problem. OCI is characterized by presence of HCV-RNA in the liver tissue only and/or Peripheral Blood Mononuclear Cells (PBMCs) with undetectable HCV-RNA or antibodies in the serum. Support for presence of this entity comes from the observation that HCV, even though a hepatotropic virus, can replicate as well at extrahepatic sites, including peripheral blood mononuclear cells. Objectives The current study evaluated the hidden infection of hepatitis C virus among patients on maintenance hemodialysis with negative ELISA and PCR by using PCR in mononuclear cells as a marker in these patients. Our study was done in Sharkia Governorate. Patients and Methods In our study, the 60 included subjects had an average age of 48.27 years with range from 19 to 77 years and included 33 males (55%) and 27 females (45%). The subjects had average dialysis duration of 6.51 ± 4.10years with range from 7 months to 19 years. Results Our study showed no statistically significant differences between occult HCV cases and negative cases in frequency of blood transfusion. There were no statistically significant differences between occult HCV cases and negative cases in splenomegaly, hepatomegaly or coarse liver but there was a statistically significant increase in frequency of severe ascites, moderate LL edema and jaundice among occult HCV cases compared to negative cases. There were no statistically significant differences between occult HCV cases and negative cases in urea, creatinine, ALT, AST or albumin. In our study total bilirubin>1.7 and direct bilirubin >0.4 were significant predictors for the presence of occult HCV in hemodialysis patients. Our study showed that there were no statistically significant correlation between viral load in PBMCs and laboratory findings among occult HCV cases. Conclusion Hemodialysis patients are liable for occult HCV infections with a considerable impact on their health. Total and direct bilirubin are predictors of presence of occult hepatitis C infection at cut-off values of 1.7 mg/dl and 0.4 mg/dl respectively.

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