scholarly journals Correlation between synovial fluid calcium containing crystal estimation and varying grades of osteoarthritis created using a rabbit model: Potential diagnostic tool

2020 ◽  
Vol 11 ◽  
pp. S506-S511
Author(s):  
Elizabeth Vinod ◽  
Tephilla Epsibha Jefferson ◽  
Soosai Manickam Amirtham ◽  
Neetu Prince ◽  
Tulasi Geevar ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Diagnostic Tool ◽  
Rabbit Model ◽  
Model Potential

Age and multiparity related urethral sphincter muscle dysfunction in a rabbit model: Potential roles of TGF‐β and Wnt‐β catenin signaling pathways

2018 ◽  
Vol 38 (2) ◽  
pp. 607-614 ◽  
Author(s):  
Mahadevan R. Rajasekaran ◽  
Johnny Fu ◽  
My‐Uyen (Lilly) Nguyen ◽  
Yaozhi Wang ◽  
Michael Albo ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Rabbit Model ◽  
Model Potential ◽  
Muscle Dysfunction ◽  
Sphincter Muscle ◽  
Urethral Sphincter

Characterization of urethral fibrosis in a rabbit model: Potential roles of Wnt‐β catenin pathway and epithelial to mesenchymal transition

2020 ◽  
Vol 39 (2) ◽  
pp. 625-632
Author(s):  
Jesse W. Tai ◽  
Hosong Yu ◽  
Abinav T. Chilukuri ◽  
Raag Bhargava ◽  
Rucha Deshpande ◽  
...  
Keyword(s):  
Epithelial To Mesenchymal Transition ◽  
Rabbit Model ◽  
Model Potential ◽  
Mesenchymal Transition

scholarly journals Titania-Containing Bone Cement Shows Excellent Osteoconductivity in A Synovial Fluid Environment and Bone-Bonding Strength in Osteoporosis

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1110
Author(s):  
Tomotoshi Kawata ◽  
Koji Goto ◽  
Masashi Imamura ◽  
Yaichiro Okuzu ◽  
Toshiyuki Kawai ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Bone Cement ◽  
Sodium Succinate ◽  
Fluid Pressure ◽  
Rabbit Model ◽  
Femoral Diaphysis ◽  
Methylprednisolone Sodium Succinate ◽  
Fluid Environment ◽  
Total Knee

Titania bone cement (TBC) reportedly has excellent in vivo bioactivity, yet its osteoconductivity in synovial fluid environments and bone-bonding ability in osteoporosis have not previously been investigated. We aimed to compare the osteoconductivity of two types of cement in a synovial fluid environment and determine their bone-bonding ability in osteoporosis. We implanted TBC and commercial polymethylmethacrylate bone cement (PBC) into rabbit femoral condyles and exposed them to synovial fluid pressure. Rabbits were then euthanized at 6, 12, and 26 weeks, and affinity indices were measured to evaluate osteoconductivity. We generated a rabbit model of osteoporosis through bilateral ovariectomy (OVX) and an 8-week treatment with methylprednisolone sodium succinate (PSL). Pre-hardened TBC and PBC were implanted into the femoral diaphysis of the rabbits in the sham control and OVX + PSL groups. Affinity indices were significantly higher for TBC than for PBC at 12 weeks (40.9 ± 16.8% versus 24.5 ± 9.02%) and 26 weeks (40.2 ± 12.7% versus 21.2 ± 14.2%). The interfacial shear strength was significantly higher for TBC than for PBC at 6 weeks (3.69 ± 1.89 N/mm2 versus 1.71 ± 1.23 N/mm2) in the OVX + PSL group. These results indicate that TBC is a promising bioactive bone cement for prosthesis fixation in total knee arthroplasty, especially for osteoporosis patients.

scholarly journals Effects of adenovirus-mediated knockdown of IRAK4 on synovitis in the osteoarthritis rabbit model

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Muzhe Li ◽  
Huiyun Li ◽  
Xun Ran ◽  
Han Yin ◽  
Xuling Luo ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Cruciate Ligament ◽  
Interleukin 1 ◽  
Cartilage Damage ◽  
Rabbit Model ◽  
Joint Disease ◽  
Control Group ◽  
Model Group ◽  
Tnf Α ◽  
And Cartilage

Abstract Background The use of interleukin-1 receptor-associated kinase 4 (IRAK4) inhibitor as a treatment for the inflammatory joint disease is a promising method. However, its underlying mechanism in osteoarthritis (OA) remains unclear. The purpose of this study is to look into the effects of adenovirus-mediated knockdown of IRAK4 on synovitis in the OA rabbit model. Methods Ad-shIRAK4 was injected two weeks after anterior cruciate ligament resection. Six weeks later, the rabbits were killed. The expression of IRAK4, TNFR-associated factor 6(TRAF6), TGF-activated kinase 1(TAK1), p-IKB kinase (p-IKK), p-nuclear factor kappa-B (p-NFκB), p38, and p-p38 in the synovial membrane was detected by western blot, qRT-PCR, and immunohistochemistry analysis. Immunohistochemistry was to detect the expression of IRAK4 proteins in articular cartilage. H&E staining was to assess the pathological changes of synovium and cartilage. The levels of interleukin (IL)-1β, tumor necrosis factor-α(TNF-α), and MMP-13 in the synovial fluid were measured by ELISA. X-ray and micro-computerized tomography (μCT) scans were used to assess knee joint conditions and microstructure of subchondral bone. Results IRAK4 expression levels in synovial tissues of the OA model group exhibited a significant upward trend. Ad-shIRAK4 significantly reduced IRAK4 mRNA expression in synovium tissues. Notably, Ad-shIRAK4 suppressed the Toll-like receptor/interleukin-1 receptor (TLR/IL-1R) signaling. In addition, in the Ad-shIRAK4 treatment group, we can see less inflammatory cell infiltration and reduced hyperplasia and angiogenesis. The levels of IL-1β, TNF-α, and MMP-13 in the synovial fluid in the OA model group were significantly higher than that in the control group, which were reduced by Ad-shIRAK4 treatment. Finally, Results of HE stains, immunohistochemistry, and μCT showed that Ad-shIRAK4 treatment has a protective effect on cartilage damage. Conclusions IRAK4 is significantly upregulated in the synovium from the osteoarthritis rabbit model. In addition, Ad-shIRAK4 reduced the expression of IRAK4 and suppressed TLR/IL-1R signaling in the synovium from the osteoarthritis rabbit model. Ad-shIRAK4 could alleviate synovitis and cartilage degradation in the osteoarthritis rabbit model, and thus alleviate the symptoms of OA and prevent the progression of OA. Graphical abstract

scholarly journals Detection of bacterial DNA in synovial fluid in dogs with arthritis: a comparison between bacterial culture and 16S rRNA polymerase chain reaction

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Alexandra Vilén ◽  
Bo Nilson ◽  
Ann-Cathrine Petersson ◽  
Mariana Cigut ◽  
Christel Nielsen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Synovial Fluid ◽  
16S Rrna ◽  
Diagnostic Tool ◽  
Bacterial Culture ◽  
Clinical Signs ◽  
Blood Agar ◽  
Control Group ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Background Septic arthritis (SA) is a serious condition in dogs that requires a prompt diagnosis and treatment to minimize long-term joint pathology. Although bacterial detection in synovial fluid (SF) through culture or cytology is often performed to confirm diagnosis, the sensitivity of these tests is low. The need for a reliable diagnostic tool to confirm the presence of bacteria in SF in humans has led to the increased use of 16S rRNA (i.e., ribosomal RNA) gene sequencing by polymerase chain reaction (16S rRNA PCR). The aim of this prospective clinical study was to compare the sensitivity and specificity of 16S rRNA PCR with bacterial culture on blood agar plates after pre-incubation of SF in paediatric blood bacterial culture bottles to identify bacteria in dogs with clinical signs of SA and to investigate the usefulness of these methods as diagnostic tools. Results Ten dogs with clinical signs of SA, nine with osteoarthritis (OA, control group) and nine with clinical signs of immune-mediated polyarthritis (IMPA, second control group) were examined. Bacterial culture was positive in seven of 10 dogs with clinical SA, of which only two were positive by 16S rRNA PCR. The sensitivity of 16S rRNA PCR and bacterial culture analysis for dogs with clinical SA were 20% and 70%, respectively. All SF samples collected from control group (n = 9) and second control group (n = 14) animals were negative on culture, and 16S rRNA PCR rendered a specificity of 100%. Conclusions Our study showed a lower sensitivity of 16S rRNA PCR than bacterial culture for dogs with clinical SA. Our findings suggest that there is currently no advantage in using 16S rRNA PCR as a diagnostic tool for dogs with clinical SA. Furthermore, our study indicates that pre-incubation in paediatric blood bacterial culture bottles before bacterial cultivation on blood agar plates might enhance bacterial culture sensitivity compared to other culture methods.

The Elicited Language Analysis Procedure

1982 ◽  
Vol 13 (1) ◽  
pp. 37-41
Author(s):  
Larry J. Mattes
Keyword(s):  
Diagnostic Tool ◽  
Descriptive Analysis ◽  
Quality Measures ◽  
Analysis Procedure ◽  
Elicited Imitation ◽  
Response Quality ◽  
Semantic Relationships ◽  
Language Analysis

Elicited imitation tasks are frequently used as a diagnostic tool in evaluating children with communication handicaps. This article presents a scoring procedure that can be used to obtain an in-depth descriptive analysis of responses produced on elicited imitation tasks. The Elicited Language Analysis Procedure makes it possible to systematically evaluate responses in terms of both their syntactic and semantic relationships to the stimulus sentences presented by the examiner. Response quality measures are also included in the analysis procedure.

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