Development and validation of a sensitive HPLC–ESI-MS/MS method for the direct determination of glucosamine in human plasma

2006 ◽  
Vol 844 (1) ◽  
pp. 119-126 ◽  
Author(s):  
A RODA ◽  
L SABATINI ◽  
A BARBIERI ◽  
M GUARDIGLI ◽  
M LOCATELLI ◽  
...  
Keyword(s):  
Human Plasma ◽  
Direct Determination ◽  
Esi Ms ◽  
Development And Validation

scholarly journals Development and Validation of an LC–ESI-MS Method for Quantitative Determination of Aripiprazole in Human Plasma and an Application to Pharmacokinetic Study

2012 ◽  
Vol 50 (10) ◽  
pp. 893-901 ◽  
Author(s):  
Sreedasyam Ravinder ◽  
Akula Tukaram Bapuji ◽  
Khagga Mukkanti ◽  
Datla Rama Raju ◽  
Hamsa Laxmi Venkata Ravikiran ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Human Plasma ◽  
Pharmacokinetic Study ◽  
Esi Ms ◽  
Development And Validation

Development and Validation of LC-ESI-MS/MS Method for the Determination of Alfuzosin in Human Plasma

2021 ◽  
Vol 76 (11) ◽  
pp. 1327-1335
Author(s):  
Moustapha E. Moustapha ◽  
Rania M. El Gamal ◽  
Mehnaz Kamal
Keyword(s):  
Human Plasma ◽  
Esi Ms ◽  
Development And Validation

Development and Validation of a Liquid Chromatography/Mass Spectrometry Method for the Simultaneous Quantitation of Rosuvastatin and Ezetimibe in Human Plasma

2013 ◽  
Vol 96 (2) ◽  
pp. 307-312 ◽  
Author(s):  
Susheel John Varghese ◽  
Ravi Thengungal Kochupappy
Keyword(s):  
Human Plasma ◽  
Internal Standard ◽  
Liquid Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Spectrometry Method ◽  
Esi Ms ◽  
Standard Curve ◽  
Liquid Liquid Extraction ◽  
Development And Validation

Abstract A simple, rapid, and sensitive LC/electrospray ionization (ESI)-MS method was developed and validated for the simultaneous determination of rosuvastatin (ROS) and ezetimibe (EZE) in human plasma. Following liquid–liquid extraction, the analytes and an internal standard, atorvastatin (ATO), were separated using an isocratic mobile phase comprising 0.1% (v/v) formic acid–methanol (20 + 80, v/v) on an RP-C18 column. Detection was performed on a mass spectrometer by selected ion monitoring using their respective [M-H]– ions, m/z 480 for ROS, m/z 408 for EZE, and m/z 557 for ATO. For both analytes, the method was linear in the range of 0.1 to 10 ng/mL. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 4 min made it possible to determine many plasma samples/day. The validated LC/ESI-MS method can be used to study pharmacokinetics, bioavailability, and bioequivalence of combined dosage forms of ROS and EZE.

Development and validation of a HPLC-ESI-MS/MS method for the determination of 5-aminosalicylic acid and its major metabolite N-acetyl-5-aminosalicylic acid in human plasma

2008 ◽  
Vol 872 (1-2) ◽  
pp. 99-106 ◽  
Author(s):  
Elisabetta Pastorini ◽  
Marcello Locatelli ◽  
Patrizia Simoni ◽  
Giulia Roda ◽  
Enrico Roda ◽  
...  
Keyword(s):  
Human Plasma ◽  
Major Metabolite ◽  
Aminosalicylic Acid ◽  
Esi Ms ◽  
Development And Validation

scholarly journals Development and validation of spectrofluorimetric and LC–MS/MS methods for the determination of hesperidin in human plasma and pharmaceutical forms

2012 ◽  
Vol 77 (11) ◽  
pp. 1625-1640 ◽  
Author(s):  
Leposava Pavun ◽  
Jasmina Dimitric-Markovic ◽  
Predrag Djurdjevic ◽  
Milena Jelikic-Stankov ◽  
Daniela Djikanovic ◽  
...  
Keyword(s):  
Human Plasma ◽  
Good Accuracy ◽  
Accurate Determination ◽  
Direct Determination ◽  
Strong Emission ◽  
Linearity Range ◽  
Spectrofluorometric Method ◽  
Development And Validation ◽  
Good Agreement

The spectrofluorometric method, based on fluorescence properties of aluminium (III)?hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows strong emission in the presence of surfactant betain sulfonate SB 12 at 476 nm with excitation at 390 nm. Linearity range in pharmaceutical forms of hesperidin was 0.06 ? 24.4 ?g mL-1 with LOD 0.016 ?g mL-1 and LOQ 0.049 ?g mL-1. Recovery values in the range 99.3 ? 99.7% indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1 ? 12.2 ?g mL-1. The LOD was 0.032 ?g mL-1 while LOQ was 0.096 ?g mL-1. Recovery values were in the range 98.4 ? 99.8%. The reliability of the method was checked by LC-MS/MS method for plasma samples and HPLC/UV method for tablets with direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 ?g mL-1. The LOD was 0.01 ?g mL-1 and LOQ was 0.03 ?g mL-1. Linearity range in plasma determination of hesperidin was 0.02 ? 10.00 ?g mL-1 with LOD 0.005 ?g mL-1 and LOQ 0.015 ?g mL-1. Good agreement between two methods indicate the usability of the proposed spectroflurometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories.

Development and validation of a UPLC-ESI-MS/MS method for the determination of N-butylscopolamine in human plasma: Application to a bioequivalence study

2012 ◽  
Vol 4 (3-4) ◽  
pp. 215-221 ◽  
Author(s):  
Wagner Alex Jann Favreto ◽  
Ana Maria Pugens Pinto ◽  
Josélia Larger Manfio ◽  
Karina Graziella Fiametti ◽  
Maycon Fernando Percio ◽  
...  
Keyword(s):  
Human Plasma ◽  
Bioequivalence Study ◽  
Esi Ms ◽  
Development And Validation
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