Development and validation of a UPLC-ESI-MS/MS method for the determination of N-butylscopolamine in human plasma: Application to a bioequivalence study

2012 ◽  
Vol 4 (3-4) ◽  
pp. 215-221 ◽  
Author(s):  
Wagner Alex Jann Favreto ◽  
Ana Maria Pugens Pinto ◽  
Josélia Larger Manfio ◽  
Karina Graziella Fiametti ◽  
Maycon Fernando Percio ◽  
...  
Keyword(s):  
Human Plasma ◽  
Bioequivalence Study ◽  
Esi Ms ◽  
Development And Validation

scholarly journals Development and Validation of an LC–ESI-MS Method for Quantitative Determination of Aripiprazole in Human Plasma and an Application to Pharmacokinetic Study

2012 ◽  
Vol 50 (10) ◽  
pp. 893-901 ◽  
Author(s):  
Sreedasyam Ravinder ◽  
Akula Tukaram Bapuji ◽  
Khagga Mukkanti ◽  
Datla Rama Raju ◽  
Hamsa Laxmi Venkata Ravikiran ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Human Plasma ◽  
Pharmacokinetic Study ◽  
Esi Ms ◽  
Development And Validation

scholarly journals Determination of Nimodipine in Human Plasma by HPLC-ESI-MS and Its Application to a Bioequivalence Study

2010 ◽  
Vol 48 (2) ◽  
pp. 81-85 ◽  
Author(s):  
Y. Zhao ◽  
D. Zhai ◽  
X. Chen ◽  
Q. Yu ◽  
H. He ◽  
...  
Keyword(s):  
Human Plasma ◽  
Bioequivalence Study ◽  
Esi Ms

Development and Validation of LC-ESI-MS/MS Method for the Determination of Alfuzosin in Human Plasma

2021 ◽  
Vol 76 (11) ◽  
pp. 1327-1335
Author(s):  
Moustapha E. Moustapha ◽  
Rania M. El Gamal ◽  
Mehnaz Kamal
Keyword(s):  
Human Plasma ◽  
Esi Ms ◽  
Development And Validation

Rapid Determination of Montelukast in Human Plasma by LC-ESI-MS/MS and Its Application to a Bioequivalence Study

2009 ◽  
Vol 42 (13) ◽  
pp. 2041-2059 ◽  
Author(s):  
Nitesh K. Patel ◽  
Gunta Subbaiah ◽  
Hiten Shah ◽  
Mohan Kundlik ◽  
Mallika Sanyal ◽  
...  
Keyword(s):  
Human Plasma ◽  
Rapid Determination ◽  
Bioequivalence Study ◽  
Esi Ms

Development and Validation of a Liquid Chromatography/Mass Spectrometry Method for the Simultaneous Quantitation of Rosuvastatin and Ezetimibe in Human Plasma

2013 ◽  
Vol 96 (2) ◽  
pp. 307-312 ◽  
Author(s):  
Susheel John Varghese ◽  
Ravi Thengungal Kochupappy
Keyword(s):  
Human Plasma ◽  
Internal Standard ◽  
Liquid Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Spectrometry Method ◽  
Esi Ms ◽  
Standard Curve ◽  
Liquid Liquid Extraction ◽  
Development And Validation

Abstract A simple, rapid, and sensitive LC/electrospray ionization (ESI)-MS method was developed and validated for the simultaneous determination of rosuvastatin (ROS) and ezetimibe (EZE) in human plasma. Following liquid–liquid extraction, the analytes and an internal standard, atorvastatin (ATO), were separated using an isocratic mobile phase comprising 0.1% (v/v) formic acid–methanol (20 + 80, v/v) on an RP-C18 column. Detection was performed on a mass spectrometer by selected ion monitoring using their respective [M-H]– ions, m/z 480 for ROS, m/z 408 for EZE, and m/z 557 for ATO. For both analytes, the method was linear in the range of 0.1 to 10 ng/mL. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 4 min made it possible to determine many plasma samples/day. The validated LC/ESI-MS method can be used to study pharmacokinetics, bioavailability, and bioequivalence of combined dosage forms of ROS and EZE.

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