scholarly journals The role of basophil activation test in allergic bronchopulmonary aspergillosis and Aspergillus fumigatus sensitization in cystic fibrosis patients

2016 ◽  
Vol 15 (5) ◽  
pp. 587-596 ◽  
Author(s):  
Anna Katelari ◽  
Marianna Tzanoudaki ◽  
Maria Noni ◽  
Maria Kanariou ◽  
Maria Theodoridou ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Aspergillus Fumigatus ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test

scholarly journals BASOPHILE ACTIVATION TEST FOR THE DIAGNOSTICS OF FUNGAL SENSITIZATION IN THE PATIENTS WITH CYSTIC FIBROSIS

2019 ◽  
Vol 21 (5) ◽  
pp. 919-928
Author(s):  
Ya. I. Kozlova ◽  
E. V. Frolova ◽  
A. E. Uchevatkina ◽  
L. V. Filippova ◽  
O. V. Aak ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Stimulation Index ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Basophil Activation ◽  
Total Ige ◽  
Activation Test ◽  
Fungal Allergens ◽  
Fungal Sensitization

Aspergillus fumigatus colonization in the patients with cystic fibrosis (CF) may cause sensitization against A. fumigatus and/or allergic bronchopulmonary aspergillosis (ABPA), which significantly worsens the course of underlying disease. At the present time, new diagnostic tests are searched for detection of fungal sensitization in these patients. The aim of this work was to evaluate an opportunity of application of basophile activation test with A. fumigatus allergen in vitro using flow cytometry, aiming for identification of fungal sensitization in the CF patients. The study included 190 patients with CF aged 1 to 37 years. All the patients underwent common allergy screening (skin tests with fungal allergens, determination of serum levels of total IgE and specific IgE for the fungal allergens), and mycological examination (microscopy and culture of respiratory substrates). Computed tomography of the chest was performed upon clinical indications. The basophil activation test with the A. fumigatus allergen was performed in 10 CF patients with ABPA, and 10 CF patients without ABPA, in addition to the standard allergological examination. Frequency of sensitization to A. fumigatus in the patients with cystic fibrosis was 27%, the incidence of allergic bronchopulmonary aspergillosis was 5.7%. The number of eosinophils, total IgE and specific IgE levels in CF patients with ABPA were significantly higher than in CF patients without ABPA. In blood of the ABPA patients we have identified 68.5 (52.5-81.5%) of basophilic leukocytes activated by A. fumigatus allergen, with a stimulation index of 17.07 (10.30-27.70). In appropriate comparison group, the stimulation index did not exceed 1.5 (p = 0.000). Direct positive correlation between the levels of specific IgE to A. fumigatus and the number of basophils activated by A. fumigatus allergens was revealed (r = 0.77; р < 0.05). FVC values and the body mass index in CF patients with ABPA were significantly lower when compared with the patients without fungal sensitization. Introduction of the basophil activation test, along with standard techniques, may enable a more differentiated assessment of ABPA development in CF patients. Timely detection of associations between A. fumigatus sensitization and clinical status of CF patients will facilitate early and effective administration of specific therapy.

scholarly journals Role of basophil activation test for monitoring the immunological changes during desnsitization to cow’s milk: a case report

2011 ◽  
Vol 1 (S1) ◽  
Author(s):  
Valentina Pecora ◽  
Eleonora Nucera ◽  
Amira Colagiovanni ◽  
Alessandro Buonomo ◽  
Tiziana De Pasquale ◽  
...  
Keyword(s):  
Case Report ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Cow’S Milk ◽  
Activation Test ◽  
Cow's Milk ◽  
Immunological Changes

Role of the basophil activation test in the diagnosis of local allergic rhinitis

2013 ◽  
Vol 132 (4) ◽  
pp. 975-976.e5 ◽  
Author(s):  
Enrique Gómez ◽  
Paloma Campo ◽  
Carmen Rondón ◽  
Esther Barrionuevo ◽  
Natalia Blanca-López ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test ◽  
Local Allergic Rhinitis

The utility of the basophil activation test in differentiating asthmatic subjects with and without allergic bronchopulmonary aspergillosis

Mycoses ◽  
2020 ◽  
Vol 63 (6) ◽  
pp. 588-595 ◽  
Author(s):  
Kuruswamy Thurai Prasad ◽  
Valliappan Muthu ◽  
Inderpaul Singh Sehgal ◽  
Sahajal Dhooria ◽  
Pawan Singh ◽  
...  
Keyword(s):  
Allergic Bronchopulmonary Aspergillosis ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test

Role Of Basophil Activation Test For Identifying Subjects With Local Allergic Rhinitis

2014 ◽  
Vol 133 (2) ◽  
pp. AB75
Author(s):  
Paloma Campo ◽  
Carmen Rondon ◽  
Enrique Gómez ◽  
Esther Barrionuevo Sanchez ◽  
Luisa Galindo ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test ◽  
Local Allergic Rhinitis

Blood basophil activation is a reliable biomarker of allergic bronchopulmonary aspergillosis in cystic fibrosis

2015 ◽  
Vol 47 (1) ◽  
pp. 177-185 ◽  
Author(s):  
Yael Gernez ◽  
Jeffrey Waters ◽  
Bojana Mirković ◽  
Gillian M. Lavelle ◽  
Colleen E. Dunn ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Ex Vivo ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Basophil Activation Test ◽  
Surface Expression ◽  
Specific Ige ◽  
Basophil Activation ◽  
Cross Sectional ◽  
Prospective Longitudinal Study ◽  
Prospective Longitudinal

The diagnosis of cystic fibrosis (CF) patients with allergic bronchopulmonary aspergillosis (ABPA) is clinically challenging, due to the absence of an objective biological test. Since blood basophils play a major role in allergic responses, we hypothesised that changes in their surface activation pattern discriminate between CF patients with and without ABPA.We conducted a prospective longitudinal study (Stanford cohort) comparing basophil activation test CD203c levels by flow cytometry before and after activation withAspergillus fumigatusallergen extract or recombinantAspf1 in 20 CF patients with ABPA (CF-ABPA) and in two comparison groups: CF patients withA. fumigatuscolonisation (AC) but without ABPA (CF-AC; n=13) and CF patients without either AC or ABPA (CF; n=12). Patients were tested every 6 months and when ill with pulmonary exacerbation. We also conducted cross-sectional validation in a separate patient set (Dublin cohort).Basophil CD203c surface expression reliably discriminated CF-ABPA from CF-AC and CF over time. Ex vivostimulation withA. fumigatusextract or recombinantAspf1 produced similar results within the Stanford (p<0.0001) and the Dublin cohorts. CF-ABPA patients were likelier to have elevated specific IgE toA. fumigatusand were less frequently co-infected withStaphylococcus aureus.Basophil CD203c upregulation is a suitable diagnostic and stable monitoring biomarker of ABPA in CF.

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