scholarly journals Fragmentation of multiply-charged intact protein ions using MALDI TOF-TOF mass spectrometry

2008 ◽  
Vol 19 (2) ◽  
pp. 231-238 ◽  
Author(s):  
Zhaoyang Liu ◽  
Kevin L. Schey
Keyword(s):  
Mass Spectrometry ◽  
Intact Protein ◽  
Maldi Tof ◽  
Multiply Charged ◽  
Protein Ions

scholarly journals Optimization in Detecting Multiply-charged Protein Ions using MALDI TOF-MS

2013 ◽  
Vol 4 (1) ◽  
pp. 21-23 ◽  
Author(s):  
Jihyeon Lee ◽  
Jangmi Hong ◽  
Taehee Kim ◽  
Jeongkwon Kim
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Multiply Charged ◽  
Protein Ions ◽  
Tof Ms

scholarly journals Proteoform-selective imaging of tissues using mass spectrometry

2021 ◽  
Author(s):  
Manxi Yang ◽  
Hang Hu ◽  
Pei Su ◽  
Paul M. Thomas ◽  
Jeannie M. Camarillo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Biological Tissues ◽  
Ionization Mass ◽  
Top Down ◽  
Tissue Sections ◽  
Spatially Resolved ◽  
Multiply Charged ◽  
Specific Analysis ◽  
Protein Ions

Unraveling the complexity of biological systems relies on the development of new approaches for spatially resolved proteoform-specific analysis of the proteome. Top-down proteomics is a powerful tool, which has been used for the identification of thousands of proteoforms in biological samples. Herein, we present a first spatially resolved top-down proteomics analysis of biological tissues using nanospray desorption electrospray ionization mass spectrometry imaging (nano-DESI MSI). Nano-DESI generates multiply charged protein ions, which is advantageous for their structural characterization using tandem mass spectrometry (MS/MS). Proof-of-concept experiments demonstrate that the nano-DESI MSI combined with on-tissue top-down proteomics is ideally suited for the proteoform-selective imaging of thin tissue sections. Using rat brain tissue as a model system, we provide the first evidence of the differential proteoform expression in different regions of the brain.

Heat-Induced Rearrangement of the Disulfide Bond of Lactoglobulin Characterized by Multiply Charged MALDI-TOF/TOF Mass Spectrometry

2018 ◽  
Vol 90 (18) ◽  
pp. 10670-10675 ◽  
Author(s):  
Lingpeng Zhan ◽  
Yu Liu ◽  
Xiaobo Xie ◽  
Caiqiao Xiong ◽  
Zongxiu Nie
Keyword(s):  
Mass Spectrometry ◽  
Disulfide Bond ◽  
Maldi Tof ◽  
Multiply Charged
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