scholarly journals Liquid AP-UV-MALDI Enables Stable Ion Yields of Multiply Charged Peptide and Protein Ions for Sensitive Analysis by Mass Spectrometry

2013 ◽  
Vol 52 (8) ◽  
pp. 2364-2367 ◽  
Author(s):  
Rainer Cramer ◽  
Alexander Pirkl ◽  
Franz Hillenkamp ◽  
Klaus Dreisewerd
Keyword(s):  
Mass Spectrometry ◽  
Sensitive Analysis ◽  
Multiply Charged ◽  
Protein Ions ◽  
Ion Yields

scholarly journals Proteoform-selective imaging of tissues using mass spectrometry

2021 ◽  
Author(s):  
Manxi Yang ◽  
Hang Hu ◽  
Pei Su ◽  
Paul M. Thomas ◽  
Jeannie M. Camarillo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Biological Tissues ◽  
Ionization Mass ◽  
Top Down ◽  
Tissue Sections ◽  
Spatially Resolved ◽  
Multiply Charged ◽  
Specific Analysis ◽  
Protein Ions

Unraveling the complexity of biological systems relies on the development of new approaches for spatially resolved proteoform-specific analysis of the proteome. Top-down proteomics is a powerful tool, which has been used for the identification of thousands of proteoforms in biological samples. Herein, we present a first spatially resolved top-down proteomics analysis of biological tissues using nanospray desorption electrospray ionization mass spectrometry imaging (nano-DESI MSI). Nano-DESI generates multiply charged protein ions, which is advantageous for their structural characterization using tandem mass spectrometry (MS/MS). Proof-of-concept experiments demonstrate that the nano-DESI MSI combined with on-tissue top-down proteomics is ideally suited for the proteoform-selective imaging of thin tissue sections. Using rat brain tissue as a model system, we provide the first evidence of the differential proteoform expression in different regions of the brain.

Ion Yields and Fragmentation Processes in the Radiolysis of HCl and DCl

1974 ◽  
Vol 52 (3) ◽  
pp. 359-364 ◽  
Author(s):  
Peter F. Carr ◽  
Patricia Christensen ◽  
Derek E. Wilson ◽  
David A. Armstrong
Keyword(s):  
Mass Spectrometry ◽  
Electron Impact ◽  
Isotope Effect ◽  
Relative Abundance ◽  
Fast Electron ◽  
Ionization Yield ◽  
Ionization Processes ◽  
Ion Yields ◽  
Fragmentation Processes ◽  
Primary Ionization

The ionization yield from fast electron impact on DCl under radiolysis conditions at 200 to 650 Torr was found to be 1.006 ± 0.023 times that from HCl. Also primary ionization processes were investigated by mass spectrometry. The relative abundance of Cl+ from HCl near threshold at 17.4 eV was about twice that from DCl, but processes with onset at and above 21.0 eV did not exhibit an isotope effect. The interpretation of the results and their implications concerning the mechanism of radiolysis of HCl were discussed.

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