Involvement of heme oxygenase-1 induction in the cytoprotective and neuroinflammatory activities of Siegesbeckia Pubescens isolated from 5,3′-dihydroxy-3,7,4′-trimethoxyflavone in HT22 cells and BV2 cells

2016 ◽  
Vol 40 ◽  
pp. 65-72 ◽  
Author(s):  
Dong-Sung Lee ◽  
Mina Lee ◽  
Sang Hyun Sung ◽  
Gil Saeng Jeong
Keyword(s):  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Ht22 Cells ◽  
Bv2 Cells ◽  
Siegesbeckia Pubescens

scholarly journals Involvement of Heme Oxygenase-1 Induction in the Cytoprotective and Immunomodulatory Activities ofViola patriniiin Murine Hippocampal and Microglia Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Bin Li ◽  
Dong-Sung Lee ◽  
Hyun-Gyu Choi ◽  
Kyoung-Su Kim ◽  
Gil-Saeng Jeong ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heme Oxygenase ◽  
Therapeutic Potential ◽  
Reactive Oxygen Species Generation ◽  
Heme Oxygenase 1 ◽  
Related Factor ◽  
Ht22 Cells ◽  
Proinflammatory Mediators ◽  
Bv2 Cells ◽  
Anti Inflammatory

A number of diseases that lead to injury of the central nervous system are caused by oxidative stress and inflammation in the brain. In this study, NNMBS275, consisting of the ethanol extract ofViola patrinii, showed potent antioxidative and anti-inflammatory activity in murine hippocampal HT22 cells and BV2 microglia. NNMBS275 increased cellular resistance to oxidative injury caused by glutamate-induced neurotoxicity and reactive oxygen species generation in HT22 cells. In addition, the anti-inflammatory effects of NNMBS275 were demonstrated by the suppression of proinflammatory mediators, including proinflammatory enzymes (inducible nitric oxide synthase and cyclooxygenase-2) and cytokines (tumor necrosis factor-αand interleukin-1β). Furthermore, we found that the neuroprotective and anti-inflammatory effects of NNMBS275 were linked to the upregulation of nuclear transcription factor-E2-related factor 2-dependent expression of heme oxygenase-1 in HT22 and BV2 cells. These results suggest that NNMBS275 possesses therapeutic potential against neurodegenerative diseases that are induced by oxidative stress and neuroinflammation.

scholarly journals The Anti-Oxidative and Anti-Neuroinflammatory Effects of Sargassum horneri by Heme Oxygenase-1 Induction in BV2 and HT22 Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 859
Author(s):  
Wonmin Ko ◽  
Hwan Lee ◽  
Nayeon Kim ◽  
Hee Geun Jo ◽  
Eun-Rhan Woo ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Soluble Fraction ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Soluble ◽  
Sargassum Horneri ◽  
Heme Oxygenase 1 ◽  
Related Factor ◽  
Ht22 Cells ◽  
Bv2 Cells

Sargassum horneri is used as a traditional medicinal agent and exhibits various pharmacological effects. In this study, we found that the 70% EtOH extract contained 34.37 ± 0.75 μg/mg fucosterol. We tested the antioxidant activities of the 70% EtOH extracts and their fractions. The CH2Cl2-soluble fraction showed the strongest DPPH and ABTS radical scavenging activities. Next, we evaluated the anti-neuroinflammatory effects of S. horneri on lipopolysaccharide (LPS)-stimulated BV2 cells. Pretreatment with the extract and fractions suppressed LPS-induced production of nitric oxide (NO) in BV2 cells. The 70% EtOH, CH2Cl2-soluble fraction, and water-soluble fraction inhibited the production of prostaglandin E2, interleukin-6, and tumor necrosis factor-α, as well as markedly blocking LPS-induced expression of inducible NO synthase and cyclooxygenase-2 via inactivation of the nuclear factor-kappa B pathway. In addition, the CH2Cl2-soluble fraction showed the most remarkable heme oxygenase (HO)-1 expression effects and increased nuclear erythroid 2-related factor translocation in the nucleus. In HT22 cells, the CH2Cl2-soluble fraction inhibited cell damage and ROS production caused by glutamate via the regulation of HO-1. Therefore, CH2Cl2-soluble fractions of S. horneri can attenuate oxidative action and neuroinflammatory responses via HO-1 induction, demonstrating their potential in the treatment of neuroinflammatory diseases.

scholarly journals Taraxacum coreanum protects against glutamate-induced neurotoxicity through heme oxygenase-1 expression in mouse hippocampal HT22 cells

2017 ◽  
Vol 15 (4) ◽  
pp. 2347-2352 ◽  
Author(s):  
Chi-Su Yoon ◽  
Wonmin Ko ◽  
Dong-Sung Lee ◽  
Dong-Cheol Kim ◽  
Jongsu Kim ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Ht22 Cells ◽  
Taraxacum Coreanum

Neuroprotective Effect of Halophyte Salicornia herbacea L. Is Mediated by Activation of Heme Oxygenase-1 in Mouse Hippocampal HT22 Cells

2017 ◽  
Vol 20 (2) ◽  
pp. 140-151 ◽  
Author(s):  
Min Sun Kim ◽  
Ji Yeon Seo ◽  
Jisun Oh ◽  
Yu Kyung Jang ◽  
Choong Hwan Lee ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Neuroprotective Effect ◽  
Heme Oxygenase 1 ◽  
Ht22 Cells ◽  
Salicornia Herbacea

scholarly journals Neuroprotective and Anti-Inflammatory Effects of Kuwanon C from Cudrania tricuspidata Are Mediated by Heme Oxygenase-1 in HT22 Hippocampal Cells, RAW264.7 Macrophage, and BV2 Microglia

2020 ◽  
Vol 21 (14) ◽  
pp. 4839 ◽  
Author(s):  
Wonmin Ko ◽  
Chi-Su Yoon ◽  
Kwan-Woo Kim ◽  
Hwan Lee ◽  
Nayeon Kim ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Stress Responses ◽  
Heme Oxygenase 1 ◽  
Related Factor ◽  
Neuroprotective Effects ◽  
Bv2 Cells ◽  
Bv2 Microglia ◽  
Anti Inflammatory ◽  
Nrf2 Expression ◽  
Raw264.7 Macrophage

Heme oxygenase (HO)-1 is a detoxifying phase II enzyme that plays a role in both inflammatory and oxidative stress responses. Curdrania tricuspidata is widespread throughout East Asia and is used as a therapeutic agent in traditional medicine. We investigated whether treatment with sixteen flavonoid or xanthone compounds from C. tricuspidata could induce HO-1 expression in HT22 hippocampal cells, RAW264.7 macrophage, and BV2 microglia. In these compounds, kuwanon C showed the most remarkable HO-1 expression effects. In addition, treatment with kuwanon C reduced cytoplasmic nuclear erythroid 2-related factor (Nrf2) expression and increased Nrf2 expression in the nucleus. Significant inhibition of glutamate-induced oxidative injury and induction of reactive oxygen species (ROS) occurred when HT22 hippocampal cells were pretreated with kuwanon C. The levels of inflammatory mediator and cytokine, which increased following lipopolysaccharide (LPS) stimulation, were suppressed in RAW264.7 macrophage and BV2 microglia after kuwanon C pretreatment. Kuwanon C also attenuated p65 DNA binding and translocation into the nucleus in LPS-induced RAW264.7 and BV2 cells. The anti-inflammatory, anti-neuroinflammatory, and neuroprotective effects of kuwanon C were reversed when co-treatment with HO-1 inhibitor of tin protoporphyrin-IX (SnPP). These results suggest that the neuroprotective and anti-inflammatory effects of kuwanon C are regulated by HO-1 expression.

Neuroprotective Effects of Kinetin Against Glutamate-Induced Oxidative Cytotoxicity in HT22 Cells: Involvement of Nrf2 and Heme Oxygenase-1

2017 ◽  
Vol 33 (4) ◽  
pp. 725-737 ◽  
Author(s):  
Yunpeng Wei ◽  
Dan Liu ◽  
Yin Zheng ◽  
Chaoshuang Hao ◽  
Honglian Li ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Neuroprotective Effects ◽  
Ht22 Cells

Anti-inflammatory activity of xanthohumol involves heme oxygenase-1 induction via NRF2-ARE signaling in microglial BV2 cells

2011 ◽  
Vol 58 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Ik-Soo Lee ◽  
Juhee Lim ◽  
Jiyeong Gal ◽  
Jeen Chu Kang ◽  
Hyun Jung Kim ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Inflammatory Activity ◽  
Heme Oxygenase 1 ◽  
Bv2 Cells ◽  
Anti Inflammatory
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