Curcumin induces apoptosis in tumor necrosis factor-alpha-treated HaCaT cells

2012 ◽  
Vol 13 (2) ◽  
pp. 170-174 ◽  
Author(s):  
Jun Sun ◽  
Jinzhao Han ◽  
Yi Zhao ◽  
Quangang Zhu ◽  
Jinhong Hu
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Hacat Cells ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Necrosis Factor

scholarly journals Synergistic Effects of Photo-Irradiation and Curcumin-Chitosan/Alginate Nanoparticles on Tumor Necrosis Factor-Alpha-Induced Psoriasis-Like Proliferation of Keratinocytes

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1388 ◽  
Author(s):  
Clinton Gomez ◽  
Chawanphat Muangnoi ◽  
Feaungthit Sorasitthiyanukarn ◽  
Jongkonnee Wongpiyabovorn ◽  
Pornchai Rojsitthisak ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Blue Light ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Light Irradiation ◽  
Hacat Cells ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Factor Alpha ◽  
Necrosis Factor

Psoriasis is a chronic inflammatory skin disease characterized by hyperproliferation of the epidermal cells and is clinically presented as thick, bright red to pink plaques with a silvery scale. Photodynamic therapy (PDT) using visible light has become of increasing interest in the treatment of inflammatory skin diseases. In this study, we demonstrate that a combination of curcumin-loaded chitosan/alginate nanoparticles (Cur-CS/Alg NPs) and blue light emitting diodes (LED) light irradiation effectively suppressed the hyperproliferation of tumor necrosis factor-alpha (TNF-α)-induced cultured human kerlatinocyte (HaCaT) cells. The Cur-CS/Alg NPs were fabricated by emulsification of curcumin in aqueous sodium alginate solution and ionotropic gelation with calcium chloride and chitosan using an optimized formulation derived from a Box-Behnken design. The fabricated Cur-CS/Alg NPs were characterized for their particle size, zeta potential, encapsulation efficiency, and loading capacity. The surrogate 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, to measure the relative number of viable cells, showed that the CS/Alg NPs were nontoxic to normal HaCaT cells, while 0.05 µg/mL and 0.1 µg/mL of free curcumin and Cur-CS/Alg NPs inhibited the hyperproliferation of HaCaT cells induced by TNF-α. However, the Cur-CS/Alg NPs demonstrated a stronger effect than the free curcumin, especially when combined with blue light irradiation (10 J/cm2) from an LED-based illumination device. Therefore, the Cur-CS/Alg NPs with blue LED light could be potentially developed into an effective PDT system for the treatment of psoriasis.

Camptothecin Fails to Induce Apoptosis in Tumor Necrosis Factor-Alpha-Treated HaCaT Cells

Pharmacology ◽  
2012 ◽  
Vol 89 (1-2) ◽  
pp. 58-63 ◽  
Author(s):  
Jun Sun ◽  
Jinzhao Han ◽  
Quangang Zhu ◽  
Zhen Li ◽  
Jinhong Hu
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Hacat Cells ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Necrosis Factor
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