Nasal immunization with different forms of heat shock protein-65 reduced high-cholesterol-diet-driven rabbit atherosclerosis

2012 ◽  
Vol 13 (1) ◽  
pp. 82-87 ◽  
Author(s):  
Jun Long ◽  
Jie Lin ◽  
Xin Yang ◽  
Dongping Yuan ◽  
Jie Wu ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Nasal Immunization ◽  
Heat Shock Protein 65

Oral Tolerization with Mycobacterial Heat Shock Protein 65 Reduces Chronic Experimental Atherosclerosis in Aged Mice

Gerontology ◽  
2017 ◽  
Vol 64 (1) ◽  
pp. 36-48 ◽  
Author(s):  
Cecilia Wick ◽  
Elisabeth Onestingel ◽  
Egon Demetz ◽  
Hermann Dietrich ◽  
Georg Wick
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
T Regulatory Cells ◽  
Stress Protein ◽  
Experimental Atherosclerosis ◽  
Chronic Inflammatory Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Cholesterol Diet ◽  
Normal Chow ◽  
Heat Shock Protein 65

Background: Atherosclerosis is a chronic inflammatory disease of the artery wall where both innate and adaptive immunity play important roles. Modulation of the immune response against the stress protein antigen, heat shock protein (HSP) 60, by administration of mycobacterial HSP65 (mbHSP65) orally and/or nasally shows promising therapeutic results in young animals in the sense of less severe experimental atherosclerosis; however, the case of aged animals with already established atherosclerosis has so far never been investigated. Objective: To investigate if mbHSP65 immunization would further accelerate atherosclerotic progression in aged ApoE-/- mice (18 months old) with already long-established atherosclerosis and if these mice could be orally tolerized against mbHSP65. Methods: Aged wild-type (WT) and ApoE-/- mice (65 weeks) were immunized and/or orally treated with mbHSP65 and then either kept on normal chow or changed to high-cholesterol diet (HCD). Atherosclerosis was assessed by en face analysis and the number of CD4+CD25+FoxP3+ T regulatory cells (Tregs) was assessed by flow cytometry in lymph node and spleen cells. Total cholesterol and triglyceride levels were determined. Soluble mammalian HSP60 and anti-mouse HSP60 (mHSP60) and anti-mbHSP65 antibodies were detected by enzyme-linked immunosorbent assay. Results: As expected, aged WT mice had only minor lesions in the aorta, which did not change under HCD for 14 weeks. Aged ApoE-/- mice already had large complicated plaques, which increased in size under HCD. mbHSP65 immunization led to a significant aggravation of atherosclerosis in both WT and ApoE-/- mice irrespective of the nature of their diet. This increase was accompanied by increased titers of both anti-mHSP60 and anti-mbHSP65 antibodies in the circulation. The increased plaque formation could be significantly diminished with oral mbHSP65 tolerization. An increased number of Tregs and lower or unchanged levels of cholesterol and triglycerides were associated with the reduced size of aortal lesions. Conclusion: Oral tolerization against mbHSP65 could be used both to prevent and to treat chronic atherosclerosis in aged individuals.

Hyperlipidemia Induced by High Cholesterol Diet Inhibits Heat Shock Response in Rat Hearts

2002 ◽  
Vol 290 (5) ◽  
pp. 1535-1538 ◽  
Author(s):  
Tamás Csont ◽  
Gábor Balogh ◽  
Csaba Csonka ◽  
Imre Boros ◽  
Ibolya Horváth ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Response ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Rat Hearts ◽  
Shock Response

Hypolipidemic and antioxidant effect of Ajuga iva in rats fed a high-cholesterol diet

Planta Medica ◽  
2006 ◽  
Vol 72 (11) ◽  
Author(s):  
MA Lacaille-Dubois ◽  
A Chenni ◽  
DA Yahia ◽  
FO Boukortt ◽  
J Prost ◽  
...  
Keyword(s):  
Antioxidant Effect ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol

Serum lipoproteins of diabetic rats fed a high cholesterol diet

Diabetes ◽  
1980 ◽  
Vol 29 (10) ◽  
pp. 774-777 ◽  
Author(s):  
C. M. Arbeeny ◽  
D. Edelstein ◽  
S. R. Freedman ◽  
H. A. Eder
Keyword(s):  
Diabetic Rats ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
Serum Lipoproteins ◽  
High Cholesterol

Effect of Polygonum multiflorum Thunberg Extract on Lipid Metabolism in Rats Fed High-Cholesterol Diet

2012 ◽  
Vol 41 (7) ◽  
pp. 957-962 ◽  
Author(s):  
Jun-Hweok Choi ◽  
Hye-Sung Lee ◽  
Young-Eon Kim ◽  
Byoung-Mok Kim ◽  
In-Ho Kim ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Polygonum Multiflorum
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