Vaccinia virus recombinant expressing gene of tick-borne encephalitis virus non-structural NS1 protein elicits protective activity in mice

2003 ◽  
Vol 90 (2-3) ◽  
pp. 161-163 ◽  
Author(s):  
M.V Khoretonenko ◽  
M.F Vorovitch ◽  
L.G Zakharova ◽  
G.V Pashvykina ◽  
N.V Ovsyannikova ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Encephalitis Virus ◽  
Ns1 Protein ◽  
Protective Activity ◽  
Tick Borne Encephalitis ◽  
Virus Recombinant ◽  
Tick Borne Encephalitis Virus ◽  
Vaccinia Virus Recombinant

scholarly journals The NS1 protein of tick-borne encephalitis virus forms multimeric species upon secretion from the host cell

1994 ◽  
Vol 75 (12) ◽  
pp. 3453-3460 ◽  
Author(s):  
A. J. Crooks ◽  
J. M. Lee ◽  
L. M. Easterbrook ◽  
A. V. Timofeev ◽  
J. R. Stephenson
Keyword(s):  
Host Cell ◽  
Encephalitis Virus ◽  
Ns1 Protein ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

scholarly journals Immunogenicity and Protective Activity of a Chimeric Protein Based on the Domain III of the Tick-Borne Encephalitis Virus E Protein and the OmpF Porin of Yersinia pseudotuberculosis Incorporated into the TI-Complex

2018 ◽  
Vol 19 (10) ◽  
pp. 2988
Author(s):  
Nina Sanina ◽  
Natalia Chopenko ◽  
Andrey Mazeika ◽  
Ludmila Davydova ◽  
Galina Leonova ◽  
...  
Keyword(s):  
Yersinia Pseudotuberculosis ◽  
Chimeric Protein ◽  
Encephalitis Virus ◽  
Protective Activity ◽  
Ompf Porin ◽  
E Protein ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus ◽  
Domain Iii ◽  
Ti Complexes

Tick-borne encephalitis (TBE) is a widespread, dangerous infection. Unfortunately, all attempts to create safe anti-TBE subunit vaccines are still unsuccessful due to their low immunogenicity. The goal of the present work was to investigate the immunogenicity of a recombinant chimeric protein created by the fusion of the EIII protein, comprising domain III and a stem region of the tick-borne encephalitis virus (TBEV) E protein, and the OmpF porin of Yersinia pseudotuberculosis (OmpF-EIII). Adjuvanted antigen delivery systems, the tubular immunostimulating complexes (TI-complexes) based on the monogalactosyldiacylglycerol from different marine macrophytes, were used to enhance the immunogenicity of OmpF-EIII. Also, the chimeric protein incorporated into the most effective TI-complex was used to study its protective activity. The content of anti-OmpF-EIII antibodies was estimated in mice blood serum by enzyme-linked immunosorbent assay (ELISA). To study protective activity, previously immunized mice were infected with TBEV strain Dal’negorsk (GenBank ID: FJ402886). The animal survival was monitored daily for 21 days. OmpF-EIII incorporated into the TI-complexes induced about a 30–60- and 5–10-fold increase in the production of anti-OmpF-EIII and anti-EIII antibodies, respectively, in comparison with the effect of an individual OmpF-EIII. The most effective vaccine construction provided 60% protection. Despite the dramatic effect on the specific antibody titer, the studied TI-complex did not provide a statistically significant increase in the protection of OmpF-EIII protein. However, our results provide the basis of the future search for approaches to design and optimize the anti-TBEV vaccine based on the OmpF-EIII protein.

Synthetic fragments of the NS1 protein of the tick-borne encephalitis virus exhibiting a protective effect

2007 ◽  
Vol 33 (2) ◽  
pp. 213-217 ◽  
Author(s):  
T. D. Volkova ◽  
D. O. Koroev ◽  
M. A. Titova ◽  
M. B. Oboznaya ◽  
M. P. Filatova ◽  
...  
Keyword(s):  
Protective Effect ◽  
Encephalitis Virus ◽  
Ns1 Protein ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

scholarly journals Characterization of a Siberian Virus Isolated from a Patient with Progressive Chronic Tick-Borne Encephalitis

2003 ◽  
Vol 77 (1) ◽  
pp. 25-36 ◽  
Author(s):  
T. S. Gritsun ◽  
T. V. Frolova ◽  
A. I. Zhankov ◽  
M. Armesto ◽  
S. L. Turner ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genomic Sequence ◽  
Encephalitis Virus ◽  
Plaque Morphology ◽  
Amino Acid Substitutions ◽  
Ns1 Protein ◽  
Nonstructural Proteins ◽  
Dimerization Domain ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

ABSTRACT A strain of Tick-borne encephalitis virus designated Zausaev (Za) was isolated in Siberia from a patient who died of a progressive (2-year) form of tick-borne encephalitis 10 years after being bitten by a tick. The complete genomic sequence of this virus was determined, and an attempt was made to correlate the sequence with the biological characteristics of the virus. Phylogenetic analysis demonstrated that this virus belongs to the Siberian subtype of Tick-borne encephalitis virus. Comparison of Za virus with two related viruses, a Far Eastern isolate, Sofjin, and a Siberian isolate, Vasilchenko, revealed differences among the three viruses in pathogenicity for Syrian hamsters, cytopathogenicity for PS cells, plaque morphology, and the electrophoretic profiles of virus-specific nonstructural proteins. Comparative amino acid alignments revealed 10 individual amino acid substitutions in the Za virus polyprotein sequence that were different from those of other tick-borne flaviviruses. Notably, the dimeric form of the Za virus NS1 protein migrated in polyacrylamide gels as a heterogeneous group of molecules with a significantly higher electrophoretic mobility than those of the Sofjin and Vasilchenko viruses. Two amino acid substitutions, T277→V and E279→G, within the NS1 dimerization domain are probably responsible for the altered oligomerization of Za virus NS1. These studies suggest that the patient from whom Za virus was isolated died due to increased pathogenicity of the latent virus following spontaneous mutagenesis.

TBE in South Korea

2019 ◽  
Author(s):  
Joon Young Song
Keyword(s):  
South Korea ◽  
Human Case ◽  
Encephalitis Virus ◽  
Surveillance Studies ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

Although no human case of tick-borne encephalitis (TBE) has been documented in South Korea to date, surveillance studies have been conducted to evaluate the prevalence of tick-borne encephalitis virus (TBEV) in wild ticks.

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