N-Acylhomoserine lactone-dependent cell-to-cell communication and social behavior in the genus Serratia

2006 ◽  
Vol 296 (2-3) ◽  
pp. 117-124 ◽  
Author(s):  
Jun-Rong Wei ◽  
Hsin-Chih Lai
Keyword(s):  
Social Behavior ◽  
Cell Communication ◽  
Acylhomoserine Lactone ◽  
Dependent Cell

scholarly journals A LuxS-Dependent Cell-to-Cell Language Regulates Social Behavior and Development in Bacillus subtilis

2006 ◽  
Vol 188 (12) ◽  
pp. 4442-4452 ◽  
Author(s):  
Esteban Lombardía ◽  
Adrián J. Rovetto ◽  
Ana L. Arabolaza ◽  
Roberto R. Grau
Keyword(s):  
Bacillus Subtilis ◽  
Social Behavior ◽  
Quorum Sensing ◽  
Cell Communication ◽  
Model Organism ◽  
Signal Molecules ◽  
Light Production ◽  
The Social ◽  
Dependent Cell ◽  
Regulatory Feedback Loop

ABSTRACT Cell-to-cell communication in bacteria is mediated by quorum-sensing systems (QSS) that produce chemical signal molecules called autoinducers (AI). In particular, LuxS/AI-2-dependent QSS has been proposed to act as a universal lexicon that mediates intra- and interspecific bacterial behavior. Here we report that the model organism Bacillus subtilis operates a luxS-dependent QSS that regulates its morphogenesis and social behavior. We demonstrated that B. subtilis luxS is a growth-phase-regulated gene that produces active AI-2 able to mediate the interspecific activation of light production in Vibrio harveyi. We demonstrated that in B. subtilis, luxS expression was under the control of a novel AI-2-dependent negative regulatory feedback loop that indicated an important role for AI-2 as a signaling molecule. Even though luxS did not affect spore development, AI-2 production was negatively regulated by the master regulatory proteins of pluricellular behavior, SinR and Spo0A. Interestingly, wild B. subtilis cells, from the undomesticated and probiotic B. subtilis natto strain, required the LuxS-dependent QSS to form robust and differentiated biofilms and also to swarm on solid surfaces. Furthermore, LuxS activity was required for the formation of sophisticated aerial colonies that behaved as giant fruiting bodies where AI-2 production and spore morphogenesis were spatially regulated at different sites of the developing colony. We proposed that LuxS/AI-2 constitutes a novel form of quorum-sensing regulation where AI-2 behaves as a morphogen-like molecule that coordinates the social and pluricellular behavior of B. subtilis.

scholarly journals Gap Junction Dependent Cell Communication Is Modulated During Transdifferentiation of Mesenchymal Stem/Stromal Cells Towards Neuron-Like Cells

2020 ◽  
Vol 8 ◽  
Author(s):  
Nadine Dilger ◽  
Anna-Lena Neehus ◽  
Klaudia Grieger ◽  
Andrea Hoffmann ◽  
Max Menssen ◽  
...  
Keyword(s):  
Gap Junction ◽  
Stromal Cells ◽  
Cell Communication ◽  
Dependent Cell

Selective contact-dependent cell communication

Nature ◽  
1976 ◽  
Vol 264 (5588) ◽  
pp. 760-762 ◽  
Author(s):  
I. FENTIMAN ◽  
J. TAYLOR-PAPADIMITRIOU ◽  
M. STOKER
Keyword(s):  
Cell Communication ◽  
Dependent Cell

scholarly journals Myosin-dependent cell-cell communication controls synchronicity of division in acute and chronic stages of Toxoplasma gondii

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Karine Frénal ◽  
Damien Jacot ◽  
Pierre-Mehdi Hammoudi ◽  
Arnault Graindorge ◽  
Bohumil Maco ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Cell Communication ◽  
Dependent Cell ◽  
Cell Cell

scholarly journals Contact area–dependent cell communication and the morphological invariance of ascidian embryogenesis

Science ◽  
2020 ◽  
Vol 369 (6500) ◽  
pp. eaar5663 ◽  
Author(s):  
Léo Guignard ◽  
Ulla-Maj Fiúza ◽  
Bruno Leggio ◽  
Julien Laussu ◽  
Emmanuel Faure ◽  
...  
Keyword(s):  
Marine Invertebrate ◽  
Cell Communication ◽  
Light Sheet ◽  
Embryonic Cell ◽  
Cell Segmentation ◽  
Asymmetric Cell Divisions ◽  
Morphogen Gradients ◽  
Cell Lineages ◽  
Genomic Divergence ◽  
Dependent Cell

Marine invertebrate ascidians display embryonic reproducibility: Their early embryonic cell lineages are considered invariant and are conserved between distantly related species, despite rapid genomic divergence. Here, we address the drivers of this reproducibility. We used light-sheet imaging and automated cell segmentation and tracking procedures to systematically quantify the behavior of individual cells every 2 minutes during Phallusia mammillata embryogenesis. Interindividual reproducibility was observed down to the area of individual cell contacts. We found tight links between the reproducibility of embryonic geometries and asymmetric cell divisions, controlled by differential sister cell inductions. We combined modeling and experimental manipulations to show that the area of contact between signaling and responding cells is a key determinant of cell communication. Our work establishes the geometric control of embryonic inductions as an alternative to classical morphogen gradients and suggests that the range of cell signaling sets the scale at which embryonic reproducibility is observed.

Inhibition of Antimicrobial Prodigiosin Production in Serratia marcescens Using Cyclodextrin-Immobilized Microgel Beads Prepared by a Non-Contact Jet Dispensing System

2017 ◽  
Vol 863 ◽  
pp. 141-145
Author(s):  
Eri Nasuno ◽  
Chigusa Okano ◽  
Yuriko Takayama ◽  
Izumi Harano ◽  
Ken-ichi Iimura ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Communication Systems ◽  
Acyl Chain ◽  
Cell Communication ◽  
Culture Broth ◽  
Acylhomoserine Lactone ◽  
Cell Functions ◽  
Gel Beads ◽  
Calcium Alginate Gel ◽  
Opportunistic Human Pathogen

Some gram-negative bacteria possess N-acylhomoserine lactone (AHL)-mediated quorum sensing (QS) as one of the cell-to-cell communication systems for regulation of various cell functions. Increase in the AHL concentration above its threshold leads to simultaneous activation of target gene expression in each cell. The AHL can diffuse in and out of the cells through the cell membrane. Artificial reduction of the AHL concentration can inhibit the AHL complex formation with its receptor inside the cell. In this study, the AHL that diffused out into the culture broth was captured on α-cyclodextrin (CD), which possesses a hydrophobic cavity and a hydrophilic shell. The α-CD-immobilized calcium alginate gel beads, which were prepared using a non-contact jet dispenser, were immersed in the culture medium during the cell growth of Serratia marcescens AS-1, which was used as the model opportunistic human pathogen. The trapping of AHLs on immobilized α-CDs due to hydrophobic interaction of the AHL acyl-chain led to an effective inhibition of AHL-mediated prodigiosin production to approximately 2% in the presence of 27 wt% gel beads in the culture broth. The results of our study suggest that CD has high potential to regulate QS involved in matter production, biofilm formation, and virulence factor expression.

scholarly journals Genetic Analysis of the DrosophilaDNAprimGene: The Function of the 60-kD Primase Subunit of DNA Polymerase Opposes thefat facetsSignaling Pathway in the Developing Eye

Genetics ◽  
2000 ◽  
Vol 156 (4) ◽  
pp. 1787-1795 ◽  
Author(s):  
Xin Chen ◽  
Qinghong Li ◽  
Janice A Fischer
Keyword(s):  
Dna Polymerase ◽  
Dna Sequences ◽  
Compound Eye ◽  
Large Subunit ◽  
Cell Communication ◽  
Dna Primase ◽  
Morphological Defects ◽  
Dependent Cell ◽  
Fat Facets ◽  
Communication Pathway

AbstractThe Drosophila DNAprim gene encodes the large subunit (60 kD) of DNA primase, the part of DNA polymerase α that synthesizes RNA primers during DNA replication. The precise function of the 60-kD subunit is unknown. In a mutagenesis screen for suppressors of the fat facets (faf) mutant eye phenotype, we identified mutations in DNAprim. The faf gene encodes a deubiquitinating enzyme required specifically for patterning the compound eye. The DNA sequences of four DNAprim alleles were determined and these define essential protein domains. We show that while flies lacking DNAprim activity are lethal, flies with reduced DNAprim activity display morphological defects in their eyes, and unlike faf mutants, cell cycle abnormalities in larval eye discs. Mechanisms by which DNA primase levels might influence the faf-dependent cell communication pathway are discussed.

scholarly journals A Novel Quorum-Quenching N-Acylhomoserine Lactone Acylase from Acidovorax sp. Strain MR-S7 Mediates Antibiotic Resistance

2017 ◽  
Vol 83 (13) ◽  
Author(s):  
Hiroyuki Kusada ◽  
Hideyuki Tamaki ◽  
Yoichi Kamagata ◽  
Satoshi Hanada ◽  
Nobutada Kimura
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Quorum Sensing ◽  
Cell Communication ◽  
Quorum Quenching ◽  
Multidrug Resistant ◽  
Bifunctional Enzyme ◽  
Content Type ◽  
Acylhomoserine Lactone ◽  
Multidrug Resistant Bacterium

ABSTRACT N-Acylhomoserine lactone acylase (AHL acylase) is a well-known enzyme responsible for disrupting cell-cell communication (quorum sensing) in bacteria. Here, we isolated and characterized a novel and unique AHL acylase (designated MacQ) from a multidrug-resistant bacterium, Acidovorax sp. strain MR-S7. The purified MacQ protein heterologously expressed in Escherichia coli degraded a wide variety of AHLs, ranging from C6 to C14 side chains with or without 3-oxo substitutions. We also observed that AHL-mediated virulence factor production in a plant pathogen, Pectobacterium carotovorum, was dramatically attenuated by coculture with MacQ-overexpressing Escherichia coli, whereas E. coli with an empty vector was unable to quench the pathogenicity, which strongly indicates that MacQ can act in vivo as a quorum-quenching enzyme and interfere with the quorum-sensing system in the pathogen. In addition, this enzyme was found to be capable of degrading a wide spectrum of β-lactams (penicillin G, ampicillin, amoxicillin, carbenicillin, cephalexin, and cefadroxil) by deacylation, clearly indicating that MacQ is a bifunctional enzyme that confers both quorum quenching and antibiotic resistance on strain MR-S7. MacQ has relatively low amino acid sequence identity to any of the known acylases (<39%) and has among the broadest substrate range. Our findings provide the possibility that AHL acylase genes can be an alternative source of antibiotic resistance genes posing a threat to human health if they migrate and transfer to pathogenic bacteria. IMPORTANCE N-Acylhomoserine lactones (AHLs) are well-known signal molecules for bacterial cell-cell communication (quorum sensing), and AHL acylase, which is able to degrade AHLs, has been recognized as a major target for quorum-sensing interference (quorum quenching) in pathogens. In this work, we succeeded in isolating a novel AHL acylase (MacQ) from a multidrug-resistant bacterium and demonstrated that the MacQ enzyme could confer multidrug resistance as well as quorum quenching on the host organism. Indeed, the purified MacQ protein was found to be bifunctional and capable of degrading not only various AHL derivatives but also multiple β-lactam antibiotics by deacylation activities. Although quorum quenching and antibiotic resistance have been recognized to be distinct biological functions, our findings clearly link the two functions by discovering the novel bifunctional enzyme and further providing the possibility that a hitherto-overlooked antibiotic resistance mechanism mediated by the quorum-quenching enzyme may exist in natural environments and perhaps in clinical settings.

CellTalkDB: a manually curated database of ligand–receptor interactions in humans and mice

2020 ◽  
Author(s):  
Xin Shao ◽  
Jie Liao ◽  
Chengyu Li ◽  
Xiaoyan Lu ◽  
Junyun Cheng ◽  
...  
Keyword(s):  
Single Cell ◽  
Protein Interactions ◽  
Cell Communication ◽  
Systematic Investigation ◽  
Protein Protein Interactions ◽  
Biological Phenomena ◽  
Type Composition ◽  
Dependent Cell ◽  
Multicellular Organisms ◽  
Cell Cell

Abstract Cell–cell communications in multicellular organisms generally involve secreted ligand–receptor (LR) interactions, which is vital for various biological phenomena. Recent advancements in single-cell RNA sequencing (scRNA-seq) have effectively resolved cellular phenotypic heterogeneity and the cell-type composition of complex tissues, facilitating the systematic investigation of cell–cell communications at single-cell resolution. However, assessment of chemical-signal-dependent cell–cell communication through scRNA-seq relies heavily on prior knowledge of LR interaction pairs. We constructed CellTalkDB (http://tcm.zju.edu.cn/celltalkdb), a manually curated comprehensive database of LR interaction pairs in humans and mice comprising 3398 human LR pairs and 2033 mouse LR pairs, through text mining and manual verification of known protein–protein interactions using the STRING database, with literature-supported evidence for each pair. Compared with SingleCellSignalR, the largest LR-pair resource, CellTalkDB includes not only 2033 mouse LR pairs but also 377 additional human LR pairs. In conclusion, the data on human and mouse LR pairs contained in CellTalkDB could help to further the inference and understanding of the LR-interaction-based cell–cell communications, which might provide new insights into the mechanism underlying biological processes.

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