Amblyomma americanum salivary glands: double-stranded RNA-mediated gene silencing of synaptobrevin homologue and inhibition of PGE2 stimulated protein secretion

2004 ◽  
Vol 34 (4) ◽  
pp. 407-413 ◽  
Author(s):  
Shahid Karim ◽  
Vijay G Ramakrishnan ◽  
James S Tucker ◽  
Richard C Essenberg ◽  
John R Sauer
Keyword(s):  
Gene Silencing ◽  
Salivary Glands ◽  
Protein Secretion ◽  
Amblyomma Americanum ◽  
Double Stranded Rna

scholarly journals Viroids as a Tool to Study RNA-Directed DNA Methylation in Plants

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1187
Author(s):  
Michael Wassenegger ◽  
Athanasios Dalakouras
Keyword(s):  
Dna Methylation ◽  
Rna Interference ◽  
Gene Silencing ◽  
Plant Cells ◽  
Transcriptional Gene Silencing ◽  
Rnai Machinery ◽  
Double Stranded Rna ◽  
Post Transcriptional Gene Silencing ◽  
Cumulative Amount

Viroids are plant pathogenic, circular, non-coding, single-stranded RNAs (ssRNAs). Members of the Pospiviroidae family replicate in the nucleus of plant cells through double-stranded RNA (dsRNA) intermediates, thus triggering the host’s RNA interference (RNAi) machinery. In plants, the two RNAi pillars are Post-Transcriptional Gene Silencing (PTGS) and RNA-directed DNA Methylation (RdDM), and the latter has the potential to trigger Transcriptional Gene Silencing (TGS). Over the last three decades, the employment of viroid-based systems has immensely contributed to our understanding of both of these RNAi facets. In this review, we highlight the role of Pospiviroidae in the discovery of RdDM, expound the gradual elucidation through the years of the diverse array of RdDM’s mechanistic details and propose a revised RdDM model based on the cumulative amount of evidence from viroid and non-viroid systems.

scholarly journals Knockdown of LIM15/DMC1 in the mushroom Coprinus cinereus by double-stranded RNA-mediated gene silencing

Microbiology ◽  
2005 ◽  
Vol 151 (11) ◽  
pp. 3669-3678 ◽  
Author(s):  
Satoshi H. Namekawa ◽  
Kazuki Iwabata ◽  
Hiroko Sugawara ◽  
Fumika N. Hamada ◽  
Akiyo Koshiyama ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Sexual Development ◽  
Reverse Genetics ◽  
Model Organism ◽  
Coprinus Cinereus ◽  
Gene Repression ◽  
Mushroom Species ◽  
Double Stranded Rna ◽  
Chromosome Synapsis ◽  
Expression Construct

The basidiomycete Coprinus cinereus has many advantages as a model organism for studying sexual development and meiosis, but it has been difficult to investigate using reverse-genetics methods, such as gene disruption by homologous recombination. Here, gene repression by dsRNA-mediated gene silencing was tried as an alternative method for reverse-genetics studies. It was shown that transformation of the LIM15/DMC1 dsRNA expression construct (LIM15dsRNA) resulted in genomic insertion of LIM15dsRNA and paucity of the LIM15/DMC1 transcript. First, LIM15dsRNA was transformed into the homothallic strain AmutBmut to generate a homozygote in which both nuclei had a copy of LIM15dsRNA. The LIM15/DMC1-repressed strain showed abnormal homologous chromosome synapsis during meiosis. Basidiospore production was reduced to 16 % by the induction of dsRNA. However, approximately 60 % of basidiospores were viable. Next, a heterozygote was generated in which one nucleus had a copy of LIM15dsRNA. The phenotype was similar to that of the homozygote. These results are not only the first demonstration of dsRNA-mediated gene silencing in a member of the homobasidiomycete fungi, to which 90 % of mushroom species belong, but also the first successful use of a reverse-genetics approach in C. cinereus research.

scholarly journals Double-stranded RNA-mediated gene silencing of cysteine proteases (falcipain-1 and -2) of Plasmodium falciparum

2002 ◽  
Vol 45 (5) ◽  
pp. 1245-1254 ◽  
Author(s):  
Pawan Malhotra ◽  
Palakodeti V. N. Dasaradhi ◽  
Amit Kumar ◽  
Asif Mohmmed ◽  
Neema Agrawal ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Gene Silencing ◽  
Cysteine Proteases ◽  
Double Stranded Rna

scholarly journals Gene silencing: Double-stranded RNA mediated mRNA degradation and gene inactivation

Cell Research ◽  
2001 ◽  
Vol 11 (3) ◽  
pp. 181-186 ◽  
Author(s):  
Wei TANG ◽  
Xiao Yan LUO ◽  
Vanessa SANMUELS
Keyword(s):  
Gene Silencing ◽  
Mrna Degradation ◽  
Gene Inactivation ◽  
Double Stranded Rna
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