Stress-responsive expression of a glutathione S-transferase (delta) gene in waterflea Daphnia magna challenged by microcystin-producing and microcystin-free Microcystis aeruginosa

Harmful Algae ◽  
2016 ◽  
Vol 56 ◽  
pp. 1-8 ◽  
Author(s):  
Kai Lyu ◽  
Lei Gu ◽  
Bangping Li ◽  
Yichun Lu ◽  
Changcan Wu ◽  
...  
Keyword(s):  
Daphnia Magna ◽  
Microcystis Aeruginosa ◽  
Glutathione S Transferase ◽  
Delta Gene

scholarly journals Application of Biotests in Cyanobacterial Extract Toxicity Assessment

2014 ◽  
Vol 40 (3) ◽  
pp. 115-121 ◽  
Author(s):  
Anna Sierosławska ◽  
Anna Rymuszka ◽  
Tadeusz Skowroński
Keyword(s):  
Acute Toxicity ◽  
Daphnia Magna ◽  
Microcystis Aeruginosa ◽  
Tetrahymena Thermophila ◽  
Toxicity Assessment ◽  
Taxonomic Identification ◽  
Dam Reservoir ◽  
Extract Concentration ◽  
Initial Extract ◽  
Toxicity Unit

Abstract The aim of the study was to determine the toxicity of the extract obtained from the cyanobacterial cells derived from the waters of Zemborzycki dam reservoir with use of a battery of biotests. The taxonomic identification of the bloom-forming cyanobacteria revealed high abundance of Aphanizomenon flos-aquae and Dolichospermum spp. (Anabaena spp.) and in a lower degree of Microcystis aeruginosa and Planktothrix agardhii. In the extract obtained from concentrated cyanobacterial cells, hepatotoxin microcystin-LR at a concentration of 22.89 ± 3.74 μg/L and neurotoxin Antx-a at 13.02 ± 0.01 μg/L have been detected. Toxicity of the extract was evaluated with the following assays: Daphtoxkit F magna with the crustacean Daphnia magna, Thamnotoxkit F with the crustacean Thamnocephalus platyurus, Rotoxkit F with the rotifer Brachionus calyciflorus and Protoxkit F with ciliate Tetrahymena thermophila. The most sensitive organism among all studied was T. platyurus for which EC50 was estimated to be 1.2% of the initial extract concentration. On the basis of the highest obtained value of the toxicity unit (TU = 83) the studied sample was classified to the IV class, which is of high acute toxicity. Additionally, it was found that reactivity on cyanobacterial products differs greatly among organisms used in bioassays, which indicate the need for using a set of biotests.

Sub-lethal effects of Microcystis aeruginosa on Daphnia magna after an acute exposure

2000 ◽  
Vol 27 (4) ◽  
pp. 2107-2110
Author(s):  
Piedade Barros ◽  
M. Leonor Fidalgo ◽  
Amadeu M. V. M. Soares
Keyword(s):  
Daphnia Magna ◽  
Microcystis Aeruginosa ◽  
Acute Exposure ◽  
Lethal Effects

scholarly journals Acute toxic effects of the cyanobacterium Microcystis aeruginosa on Daphnia magna 1

1986 ◽  
Vol 31 (3) ◽  
pp. 497-502 ◽  
Author(s):  
Shulamit Nizan ◽  
Chanan Dimentman ◽  
Moshe Shilo
Keyword(s):  
Daphnia Magna ◽  
Microcystis Aeruginosa ◽  
Toxic Effects ◽  
Acute Toxic

scholarly journals Detrimental impacts of toxic Microcystis aeruginosa from Vietnam on life history traits of Daphnia magna

2016 ◽  
Vol 8 (1) ◽  
pp. 56-61
Author(s):  
Thi My Chi Vo ◽  
Thanh Luu Pham ◽  
Thanh Son Dao
Keyword(s):  
Life History ◽  
Daphnia Magna ◽  
Microcystis Aeruginosa ◽  
Life History Traits ◽  
Scientific Information ◽  
Reproductive Age ◽  
Viet Nam ◽  
Negative Effects ◽  
The Individual ◽  
Population Rate

In this study, we tested the long-term and negative effects of microcystin-producing cyanobacterium Microcystis aeruginosa from Vietnam on Daphnia magna under the laboratory conditions. The test organisms were fed with mixtures of green alga Scenedesmus armatus and toxic M. aeruginosa at different ratios (10% Microcystis + 90% Scenedesmus, 50% Microcystis + 50% Scenedesmus, 100% Microcystis, and 100% Scenedesmus) for over a period of 21 days. The life history traits of the organisms such as, survival, maturation, fecundity were daily recorded. Besides, the intrinsic population rate of D. magna in each treatment was also calculated based on the survivorship, the reproductive age and the clutch size of the animals. The results showed that survival, maturation and reproduction of the D. magna fed with 10, 50 and 100% M. aeruginosa was impaired. Additionally, the intrinsic population rate of the exposed D. magna was lower than that of the control. This study evidenced the adverse effects of toxic M. aeruginosa on both the individual and intrinsic population levels of D. magna. To our knowledge, this is the first report on the chronically detrimental impacts of toxic M. aeruginosa isolated from Vietnam on D. magna and contributed the scientific information on the severe influences of toxic cyanobacteria world wide. Trong bài viết này, chúng tôi nghiên cứu ảnh hưởng xấu mãn tính của loài vi khuẩn lam Microcystis aeruginosa có khả năng sản sinh độc tố microcysin từ Việt Nam lên Daphnia magna trong điều kiện phòng thí nghiệm. Sinh vật thí nghiệm được cho ăn với hỗn hợp tảo lục Scenedesmus armatus và M. aeruginosa có độc ở các tỷ lệ khác nhau (10% Microcystis + 90% Scenedesmus, 50% Microcystis + 50% Scenedesmus, 100% Microcystis, và 100% Scenedesmus) trong thời gian 21 ngày. Các đặc điểm vòng đời của sinh vật bao gồm sức sống, sự thành thục, sức sinh sản được theo dõi hàng ngày. Bên cạnh đó, tỷ lệ phát triển quần thể của D. magna trong từng lô thí nghiệm cũng được tính toán dựa vào sức sống, tuổi sinh sản và kích cỡ sinh sản của sinh vật. Kết quả cho thấy, sức sống, tuổi thành thục và sự sinh sản của D. magna cho ăn với 10, 50 và 100% M. aeruginosa bị ảnh hưởng xấu. Bên cạnh đó, tỷ lệ phát triển quần thể của D. magna trong lô phơi nhiễm thấp hơn so với đối chứng. Nghiên cứu này chứng minh ảnh hưởng xấu của M. aeruginosa có độc lên cả hai mức độ cá thể và quần thể của D. magna. Theo hiểu biết của chúng tôi, đây là báo cáo đầu tiên về ảnh hưởng xấu mãn tính của M. aeruginosa có độc phân lập từ Việt Nam lên D. magna and đóng góp thêm thông tin khoa học cho những ảnh hưởng nghiêm trọng của vi khuẩn lam có độc trên khắp thế giới.

Effects of Microcystis aeruginosa on life history of water flea Daphnia magna

2011 ◽  
Vol 29 (4) ◽  
pp. 892-897 ◽  
Author(s):  
Liping Liu ◽  
Kang Li ◽  
Taoying Chen ◽  
Xilin Dai ◽  
Min Jiang ◽  
...  
Keyword(s):  
Life History ◽  
Daphnia Magna ◽  
Microcystis Aeruginosa ◽  
Water Flea ◽  
History Of

scholarly journals Синергетическое действие пиретроидного инсектицида и энтомопатогенного гриба на Daphnia magna Straus

2017 ◽  
Vol 7 (4) ◽  
pp. 393-398
Author(s):  
Yu. A. Noskov ◽  
E. A. Chertkova ◽  
O. V. Polenogova ◽  
O. N. Yaroslavtseva
Keyword(s):  
Daphnia Magna ◽  
Combined Treatment ◽  
Dopamine Level ◽  
Glutathione S Transferase ◽  
Simultaneous Treatment ◽  
Metarhizium Robertsii ◽  
Defense Strategies ◽  
Detoxification System ◽  
Activity Of Enzymes ◽  
Lethal Doses

<p>The interaction between the entomopathogenic fungus <em>Metarhizium robertsii</em> and the pyrethroid insecticide esfenvalerate on <em>Daphnia magna</em> Straus was investigated. A synergy in the mortality of daphnids was detected after simultaneous treatment with sub-lethal doses of the fungus (1×10<sup>5</sup> conidia/ml) and esfenvalerate (0.1 mkg/l). The defense strategies of daphnids infected by fungus and treated with esfenvalerate and untreated insects were compared to investigate the mechanisms of this synergy. Activity of enzymes of the detoxification system and concentration of dopamine were measured. We have shown changes in the activities of the enzymes and dopamine concentration of daphnids under combined treatment of esfenvalerate and fungus. Fungus enhanced activity of glutathione-S-transferase and non-specific esterase but did not affect the dopamine level. Esfenvalerate inhibited the activity of enzymes in the detoxification system and cause a rise in dopamine level. We assume that the suppression of the detoxification system may be one of the reasons of synergy between <em>M. robertsii</em> and esfenvalerate.</p>

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